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protein coding gene - skb15 (SPCC16C4.08c) - p21 activated protein kinase inhibitor Skb15

Gene summary

Standard name
skb15
Systematic ID
SPCC16C4.08c
Product
p21 activated protein kinase inhibitor Skb15
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O74453
ORFeome ID
20/20B08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 675369..677181 reverse strand

Annotation

GO biological process

GO:0000463 - maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

References:

GO:0032956 - regulation of actin cytoskeleton organization

References:

GO:0070507 - regulation of microtubule cytoskeleton organization

References:

GO:0042254 - ribosome biogenesis

References:

GO:0023052 - signaling

References:

GO cellular component

GO:0005730 - nucleolus

References:

GO:0005634 - nucleus

References:

GO:0030684 - preribosome

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0004860 - protein kinase inhibitor activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0004859 - increased cell wall polysaccharide level

References:

Genotypes:

FYPO:0001019 - monopolar actin cortical patch localization during vegetative growth

References:

Genotypes:

FYPO:0001399 - normal mitotic spindle

References:

Genotypes:

FYPO:0002085 - normal vegetative cell growth

References:

Genotypes:

FYPO:0000013 - T-shaped vegetative cell with normal cell length

References:

Genotypes:

Protein features

PBO:0111751 - WD repeat protein

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000801 - abnormal actin cytoskeleton organization during vegetative growth

References:

Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0005853 - aggregated filamentous actin in cytoplasm

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0001919 - fragmented nucleus during vegetative growth

References:

Genotypes:

FYPO:0004859 - increased cell wall polysaccharide level

References:

Genotypes:

FYPO:0001406 - increased septum thickness

References:

Genotypes:

FYPO:0003227 - interphase microtubules present during mitosis

References:

Genotypes:

FYPO:0002148 - inviable after spore germination, multiple cell divisions, abnormal morphology

References:

Genotypes:

FYPO:0002170 - inviable after spore germination, multiple cell divisions, branched, elongated cell

References:

Genotypes:

FYPO:0002462 - inviable branched, elongated vegetative cell

References:

Genotypes:

FYPO:0003413 - inviable branched, elongated, multiseptate vegetative cell

References:

Genotypes:

FYPO:0001493 - inviable elongated multinucleate vegetative cell

References:

Genotypes:

FYPO:0002482 - inviable spheroid vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000733 - long mitotic spindle

References:

Genotypes:

FYPO:0000118 - multiseptate vegetative cell

References:

Genotypes:

FYPO:0003245 - telophase nuclear clustering

References:

Genotypes:

FYPO:0002437 - thick actin cables

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00400WD40WD40_rptPFAM
PS00678WD_REPEATS_1WD40_repeat_CSPROSITE_PATTERNS
PS50082WD_REPEATS_2WD40_rptPROSITE_PROFILES
PS50294WD_REPEATS_REGIONPROSITE_PROFILES
SM00564ire1_9PQQ_b-propeller_rptSMART
SM00320WD40_4WD40_rptSMART
SSF50978WD40 repeat-likeWD40_repeat_dom_sfSUPERFAMILY
G3DSA:2.130.10.10WD40/YVTN_repeat-like_dom_sfGENE3D
PTHR44675PAK1 INTERACTING PROTEIN 1PAK1-Kinase_RegulatorPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:11972773 - The p21-activated kinase, Shk1, is required for proper regulation of microtubule dynamics in the fission yeast, Schizosaccharomyces pombe.
Qyang Y et al. Mol Microbiol 2002 Apr;44(2):325-34
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:11389855 - Genetic and molecular characterization of Skb15, a highly conserved inhibitor of the fission yeast PAK, Shk1.
Kim HW et al. Mol Cell 2001 May;7(5):1095-101
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:12764130 - The kelch repeat protein, Tea1, is a potential substrate target of the p21-activated kinase, Shk1, in the fission yeast, Schizosaccharomyces pombe.
Kim H et al. J Biol Chem 2003 Aug 08;278(32):30074-82
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000051 - S. pombe keyword mapping
PMID:17308036 - The p21-activated protein kinase inhibitor Skb15 and its budding yeast homologue are 60S ribosome assembly factors.
Saveanu C et al. Mol Cell Biol 2007 Apr;27(8):2897-909
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:38051102 - Fission Yeast TORC1 Promotes Cell Proliferation through Sfp1, a Transcription Factor Involved in Ribosome Biogenesis.
Tai YT et al. Mol Cell Biol 2023 Dec 05;:1-18