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protein coding gene - sts5 (SPCC16C4.09) - cytoplasmic P body 3'-5'-exoribonuclease, Dis3L2-related

Gene summary

Standard name
sts5
Systematic ID
SPCC16C4.09
Product
cytoplasmic P body 3'-5'-exoribonuclease, Dis3L2-related
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
orb4, mok2
UniProt ID
O74454
ORFeome ID
48/48A05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 679835..684083 forward strand

Annotation

PBO:0005237 - distinct from protein kinase C pathway and osmosensing MAP-kinase pathway

References:

Comment

PBO:0005238 - target of staurosporine

Complementation

PBO:0123321 - is not functionally complemented by S. cerevisiae SSD1

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Disease association

MONDO:0009965 - Perlman syndrome

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GO biological process

GO:0000956 - nuclear-transcribed mRNA catabolic process

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GO cellular component

GO:0032153 - cell division site

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GO:0005737 - cytoplasm

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GO:0005829 - cytosol

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GO:0000932 - P-body

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GO molecular function

GO:0000175 - 3'-5'-RNA exonuclease activity

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GO:0003723 - RNA binding

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Modification

MOD:00595 - monomannosylated residue

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MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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MOD:01149 - sumoylated lysine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000198 - abnormal establishment or maintenance of actin cytoskeleton polarity during vegetative growth

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Genotypes:

FYPO:0001418 - abnormal microtubule cytoskeleton morphology during vegetative growth

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0002297 - dispersed actin cortical patch localization during mitotic interphase

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Genotypes:

FYPO:0002021 - dispersed actin cortical patch localization during vegetative growth

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0000650 - increased septation index

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0003028 - normal actin cortical patch localization during mitosis

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Genotypes:

FYPO:0001192 - normal growth on cell wall-degrading enzymes

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Genotypes:

FYPO:0000959 - normal growth on sodium dodecyl sulfate

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Genotypes:

FYPO:0007114 - normal microtubule bundle

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001966 - sensitive to aculeacin A

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Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

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Genotypes:

FYPO:0000105 - sensitive to cyclosporin A

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000113 - sensitive to staurosporine

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

FYPO:0004103 - viable spherical vegetative cell

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Genotypes:

FYPO:0002380 - viable spheroid vegetative cell

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Genotypes:

FYPO:0002106 - viable stubby vegetative cell

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Genotypes:

FYPO:0002377 - viable swollen vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00773RNBRNase_II/RPfam
PF17849OB_Dis3Dis3_CSD2Pfam
PF17877Dis3l2_C_termDis3l2-like_CPfam
SM00955RNBRNase_II/RSMART
G3DSA:2.40.50.700:FF:000002CATH-FunFam
G3DSA:2.40.50.140NA-bd_OB-foldCATH-Gene3D
G3DSA:2.40.50.690CATH-Gene3D
G3DSA:2.40.50.700CATH-Gene3D
SSF50249NA-bd_OB-foldSUPERFAMILY
PTHR23355RNR_RibonucleasePANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Glycine-richdisorder_predictionMobiDB-Glycine-rich
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.
Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:10087262 - Fission yeast alpha-glucan synthase Mok1 requires the actin cytoskeleton to localize the sites of growth and plays an essential role in cell morphogenesis downstream of protein kinase C function.
Katayama S et al. J Cell Biol 1999 Mar 22;144(6):1173-86
GO_REF:0000002 - Comments
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:26644575 - Discovery of a nucleocytoplasmic O-mannose glycoproteome in yeast.
Halim A et al. Proc Natl Acad Sci U S A 2015 Dec 22;112(51):15648-53
PMID:27474797 - Spatial control of translation repression and polarized growth by conserved NDR kinase Orb6 and RNA-binding protein Sts5.
Nuñez I et al. Elife 2016 Jul 30;5
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:1899230 - Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases.
Toda T et al. Genes Dev 1991 Jan;5(1):60-73
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:8522609 - Fission yeast cell morphogenesis: identification of new genes and analysis of their role during the cell cycle.
Verde F et al. J Cell Biol 1995 Dec;131(6 Pt 1):1529-38
PMID:9405296 - Genes that cause aberrant cell morphology by overexpression in fission yeast: a role of a small GTP-binding protein Rho2 in cell morphogenesis.
Hirata D et al. J Cell Sci 1998 Jan;111 ( Pt 2):149-59
PMID:38442865 - The fission yeast NDR kinase Orb6 and its signalling pathway MOR regulate cytoplasmic microtubule organization during the cell cycle.
Kume K et al. Open Biol 2024 Mar;14(3):230440
PMID:22306653 - Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility.
Astuti D et al. Nat Genet 2012 Feb 05;44(3):277-84
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:8886983 - The fission yeast sts5+ gene is required for maintenance of growth polarity and functionally interacts with protein kinase C and an osmosensing MAP-kinase pathway.
Toda T et al. J Cell Sci 1996 Sep;109 ( Pt 9):2331-42
PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.
Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22730331 - Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions.
Nie M et al. J Biol Chem 2012 Aug 24;287(35):29610-9
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:34282727 - Pak1 kinase controls cell shape through ribonucleoprotein granules.
Magliozzi JO et al. Elife 2021 Jul 20;10
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:7628434 - A novel protein kinase gene ssp1+ is required for alteration of growth polarity and actin localization in fission yeast.
Matsusaka T et al. EMBO J 1995 Jul 17;14(14):3325-38
PMID:42017229 - Temperature-specific regulation of the NDR kinase Orb6 by the MAPK Sty1 to promote heat stress resilience.
Doyle LP et al. J Cell Sci 2026 Apr 15;139(8)
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:7983142 - A calcineurin-like gene ppb1+ in fission yeast: mutant defects in cytokinesis, cell polarity, mating and spindle pole body positioning.
Yoshida T et al. J Cell Sci 1994 Jul;107 ( Pt 7):1725-35
PMID:8187760 - Genetic analysis of cell morphogenesis in fission yeast--a role for casein kinase II in the establishment of polarized growth.
Snell V et al. EMBO J 1994 May 01;13(9):2066-74
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12