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protein coding gene - dpb3 (SPCC16C4.22) - DNA polymerase epsilon Dpb3

Gene summary

Standard name
dpb3
Systematic ID
SPCC16C4.22
Product
DNA polymerase epsilon Dpb3
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPNCRNA.470, SPNG2185
UniProt ID
C6Y4D0
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 684677..685496 forward strand

Annotation

GO biological process

GO:0140529 - CMG complex assembly

References:

GO:0006335 - DNA replication-dependent chromatin assembly

References:

GO:1902983 - DNA strand elongation involved in mitotic DNA replication

References:

GO:0040029 - epigenetic regulation of gene expression

References:

GO:1902975 - mitotic DNA replication initiation

References:

GO cellular component

GO:0008622 - epsilon DNA polymerase complex

References:

GO:0043596 - nuclear replication fork

References:

GO molecular function

GO:0030337 - DNA polymerase processivity factor activity

References:

GO:0031491 - nucleosome binding

References:

Modification

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0003217 - decreased chromatin silencing at centromere central core

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0007478 - decreased epigenetic heterochromatin inheritance

References:

Genotypes:

FYPO:0004742 - normal chromatin silencing at centromere outer repeat

References:

Genotypes:

Protein features

PBO:0111763 - histone fold

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0000452 - abnormal protein localization to chromatin during vegetative growth

References:

Genotypes:

FYPO:0007376 - abolished epigenetic heterochromatin inheritance

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0003217 - decreased chromatin silencing at centromere central core

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0002827 - decreased chromatin silencing at silent mating-type cassette

References:

Genotypes:

FYPO:0004604 - decreased chromatin silencing at subtelomere

References:

Genotypes:

FYPO:0007478 - decreased epigenetic heterochromatin inheritance

References:

Genotypes:

FYPO:0000890 - decreased histone H3-K9 trimethylation at centromere outer repeat during vegetative growth

References:

Genotypes:

FYPO:0002386 - decreased protein localization to pericentric heterochromatin at centromere outer repeat region

References:

Genotypes:

FYPO:0000614 - increased duration of mitotic S phase

References:

Genotypes:

FYPO:0000893 - increased histone H3-K9 acetylation at centromere during vegetative growth

References:

Genotypes:

FYPO:0004742 - normal chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0091924 - dpb3 was previously considered to be SPAC17G8.03c but this new gene product appears to be the true ortholog of DPB3/POLE4 see TreeFam:TF103009 and PMID:29109278

PBO:0000406 - previously annotated as ncRNA

Protein features

IDNameInterPro nameDB name
PF00808CBFD_NFYB_HMFCBFA_NFYB_domainPFAM
cd23645HFD_Dpb3-likeCDD
SSF47113Histone-foldHistone-foldSUPERFAMILY
G3DSA:1.10.20.10Histone, subunit AHistone-foldGENE3D
PTHR10252HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATEDTranscr_DNA_Rep_RegPANTHER

Orthologs

References / Literature

PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
TreeFam:TF103009 - Unknown title
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:18488015 - Dynamic repertoire of a eukaryotic transcriptome surveyed at single-nucleotide resolution.
Wilhelm BT et al. Nature 2008 Jun 26;453(7199):1239-43
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:29109278 - Coordinated regulation of heterochromatin inheritance by Dpb3-Dpb4 complex.
He H et al. Proc Natl Acad Sci U S A 2017 Nov 21;114(47):12524-12529
PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
PMID:40861011 - Faithful inheritance: Parental histone recycling and epigenetic memory in fission yeast.
Fang Y et al. Cell Insight 2025 Oct;4(5):100275
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.
Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53
PMID:38479839 - Coordination of histone chaperones for parental histone segregation and epigenetic inheritance.
Fang Y et al. Genes Dev 2024 Mar 13;
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6