PomBase home

protein coding gene - ppe1 (SPCC1739.12) - serine/threonine protein phosphatase PP6 catalytic subunit Ppe1

Gene summary

Standard name
ppe1
Systematic ID
SPCC1739.12
Product
serine/threonine protein phosphatase PP6 catalytic subunit Ppe1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
esp1, ppx1, ekc2
UniProt ID
P36614
ORFeome ID
12/12G06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 2053511..2057069 forward strand

Annotation

PBO:0000610 - 3.1.3.16

Complementation

PBO:0016588 - functionally complemented by S. cerevisiae SIT4

References:

GO biological process

GO:0000082 - G1/S transition of mitotic cell cycle

References:

GO:2001211 - negative regulation of isopentenyl diphosphate biosynthetic process, mevalonate pathway

References:

GO:1903432 - regulation of TORC1 signaling

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO:0008287 - protein serine/threonine phosphatase complex

References:

GO molecular function

GO:0004721 - phosphoprotein phosphatase activity

References:

GO:0005515 - protein binding

References:

GO:0004722 - protein serine/threonine phosphatase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0001916 - elongated mononucleate vegetative cell

References:

Genotypes:

FYPO:0002430 - inviable after spore germination, multiple cell divisions

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0005450 - normal growth on okadaic acid

References:

Genotypes:

FYPO:0001238 - normal growth on staurosporine

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

FYPO:0002106 - viable stubby vegetative cell

References:

Genotypes:

FYPO:0002110 - viable tapered vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000031 - abnormal mating

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0006549 - decreased gene expression

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0003306 - decreased mitotic index

References:

Genotypes:

FYPO:0004457 - decreased protein localization to nucleus during nitrogen starvation

References:

Genotypes:

FYPO:0002681 - increased protein phosphorylation during nitrogen starvation

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0005035 - normal protein phosphorylation during nitrogen starvation

References:

Genotypes:

FYPO:0000776 - normal protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0000113 - sensitive to staurosporine

References:

Genotypes:

FYPO:0004435 - sensitive to tingenone

References:

Genotypes:

FYPO:0004433 - sensitive to viridicatumtoxin

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

FYPO:0003241 - unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002380 - viable spheroid vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00149MetallophosCalcineurin-like_PHPPFAM
cd07415MPP_PP2A_PP4_PP6CDD
PS00125SER_THR_PHOSPHATASESer/Thr-sp_prot-phosphatasePROSITE_PATTERNS
SM00156pp2a_7Ser/Thr-sp_prot-phosphataseSMART
PR00114STPHPHTASESer/Thr-sp_prot-phosphatasePRINTS
G3DSA:3.60.21.10:FF:000005FUNFAM
SSF56300Metallo-dependent phosphatasesMetallo-depent_PP-likeSUPERFAMILY
G3DSA:3.60.21.10Metallo-depent_PP-likeGENE3D
PTHR45619SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATEDPPA2-likePANTHER

Orthologs

References / Literature

PMID:7983142 - A calcineurin-like gene ppb1+ in fission yeast: mutant defects in cytokinesis, cell polarity, mating and spindle pole body positioning.
Yoshida T et al. J Cell Sci 1994 Jul;107 ( Pt 7):1725-35
PMID:35924983 - Genetic-interaction screens uncover novel biological roles and regulators of transcription factors in fission yeast.
Chatfield-Reed K et al. G3 (Bethesda) 2022 Aug 25;12(9)
PMID:8387356 - Isolation and characterization of the fission yeast protein phosphatase gene ppe1+ involved in cell shape control and mitosis.
Shimanuki M et al. Mol Biol Cell 1993 Mar;4(3):303-13
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:18204818 - Identification of small molecules inducing apoptosis by cell-based assay using fission yeast deletion mutants.
Chung KS et al. Invest New Drugs 2008 Aug;26(4):299-307
PMID:12773390 - The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore.
Goshima G et al. EMBO J 2003 Jun 02;22(11):2752-63
PMID:21680738 - Glucose controls phosphoregulation of hydroxymethylglutaryl coenzyme A reductase through the protein phosphatase 2A-related phosphatase protein, Ppe1, and Insig in fission yeast.
Burg JS et al. J Biol Chem 2011 Aug 05;286(31):27139-46
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:31072933 - Suppressor screening reveals common kleisin-hinge interaction in condensin and cohesin, but different modes of regulation.
Xu X et al. Proc Natl Acad Sci U S A 2019 May 28;116(22):10889-10898
PMID:19371376 - Schizosaccharomyces pombe cell division cycle under limited glucose requires Ssp1 kinase, the putative CaMKK, and Sds23, a PP2A-related phosphatase inhibitor.
Hanyu Y et al. Genes Cells 2009 May;14(5):539-54
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:8389306 - Negative regulation of mitosis by the fission yeast protein phosphatase ppa2.
Kinoshita N et al. Genes Dev 1993 Jun;7(6):1059-71
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:8387358 - Interaction of the pim1/spi1 mitotic checkpoint with a protein phosphatase.
Matsumoto T et al. Mol Biol Cell 1993 Mar;4(3):337-45
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:9013334 - The novel human protein serine/threonine phosphatase 6 is a functional homologue of budding yeast Sit4p and fission yeast ppe1, which are involved in cell cycle regulation.
Bastians H et al. J Cell Sci 1996 Dec;109 ( Pt 12):2865-74
PMID:26152587 - TORC1 Regulates Developmental Responses to Nitrogen Stress via Regulation of the GATA Transcription Factor Gaf1.
Laor D et al. mBio 2015 Jul 07;6(4):e00959
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:8886983 - The fission yeast sts5+ gene is required for maintenance of growth polarity and functionally interacts with protein kinase C and an osmosensing MAP-kinase pathway.
Toda T et al. J Cell Sci 1996 Sep;109 ( Pt 9):2331-42
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:21801748 - Regulation of HMG-CoA reductase in mammals and yeast.
Burg JS et al. Prog Lipid Res 2011 Oct;50(4):403-10
PMID:7628434 - A novel protein kinase gene ssp1+ is required for alteration of growth polarity and actin localization in fission yeast.
Matsusaka T et al. EMBO J 1995 Jul 17;14(14):3325-38
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:22730331 - Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions.
Nie M et al. J Biol Chem 2012 Aug 24;287(35):29610-9
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.