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protein coding gene - ssa2 (SPCC1739.13) - Hsp70 family heat shock protein Ssa2

Gene summary

Standard name
ssa2
Systematic ID
SPCC1739.13
Product
Hsp70 family heat shock protein Ssa2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
csp6, hsp70
UniProt ID
O59855
ORFeome ID
26/26H10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 2057043..2059331 forward strand

Annotation

GO biological process

GO:0034605 - cellular response to heat

References:

GO:0042026 - protein refolding

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0140602 - nucleolar peripheral inclusion body

References:

GO:0005634 - nucleus

References:

GO:0071014 - post-mRNA release spliceosomal complex

References:

GO:0140453 - protein aggregate center

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

References:

GO:0031072 - heat shock protein binding

References:

GO:0005515 - protein binding

References:

GO:0044183 - protein folding chaperone

References:

Miscellaneous functional group

PBO:0000255 - chaperone

Modification

MOD:00060 - N-acetyl-L-serine

References:

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01149 - sumoylated lysine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0004734 - decreased misfolded protein degradation

References:

Genotypes:

FYPO:0004742 - normal chromatin silencing at centromere outer repeat

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0003157 - abnormal maintenance of protein location at growing cell tip

References:

Genotypes:

FYPO:0002859 - abolished protein localization to non-growing cell tip

References:

Genotypes:

FYPO:0001326 - altered RNA level during vegetative growth

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0003150 - decreased NETO

References:

Genotypes:

FYPO:0007299 - decreased protein aggregate center formation

References:

Genotypes:

FYPO:0000929 - decreased protein localization to cell cortex during vegetative growth

References:

Genotypes:

FYPO:0002871 - decreased protein localization to growing cell tip

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0003152 - increased protein level during cellular response to heat

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0003155 - intermittent monopolar cell growth

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0004742 - normal chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0001587 - normal protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0002013 - normal protein oxidation during vegetative growth

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001090 - resistance to heat shock during vegetative growth

References:

Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00012HSP70Hsp_70_famPfam
cd10233ASKHA_NBD_HSP70_HSPA1CDD
PS00297HSP70_1Heat_shock_70_CSPROSITE patterns
PS00329HSP70_2Heat_shock_70_CSPROSITE patterns
PS01036HSP70_3Heat_shock_70_CSPROSITE patterns
PR00301HEATSHOCK70PRINTS
G3DSA:1.20.1270.10:FF:000021CATH-FunFam
G3DSA:2.60.34.10:FF:000002CATH-FunFam
G3DSA:3.30.30.30:FF:000001CATH-FunFam
G3DSA:3.30.420.40:FF:000026CATH-FunFam
G3DSA:3.30.420.40:FF:000172CATH-FunFam
G3DSA:3.90.640.10:FF:000134CATH-FunFam
G3DSA:1.20.1270.10HSP70_C_sfCATH-Gene3D
G3DSA:2.60.34.10HSP70_peptide-bd_sfCATH-Gene3D
G3DSA:3.30.30.30CATH-Gene3D
G3DSA:3.30.420.40CATH-Gene3D
G3DSA:3.90.640.10CATH-Gene3D
SSF100920HSP70_peptide-bd_sfSUPERFAMILY
SSF100934HSP70_C_sfSUPERFAMILY
SSF53067ATPase_NBDSUPERFAMILY
PTHR19375Hsp_70_famPANTHER
NF001413dnaKNCBIFAM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity

Orthologs

References / Literature

PMID:27966061 - UBL/BAG-domain co-chaperones cause cellular stress upon overexpression through constitutive activation of Hsf1.
Poulsen EG et al. Cell Stress Chaperones 2017 Jan;22(1):143-154
PMID:23779158 - The proteasome factor Bag101 binds to Rad22 and suppresses homologous recombination.
Saito Y et al. Sci Rep 2013;3:2022
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:28765280 - The exocyst subunit Sec3 is regulated by a protein quality control pathway.
Kampmeyer C et al. J Biol Chem 2017 Sep 15;292(37):15240-15253
PMID:16278451 - Activation of AP-1-dependent transcription by a truncated translation initiation factor.
Jenkins CC et al. Eukaryot Cell 2005 Nov;4(11):1840-50
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:27664222 - A Pap1-Oxs1 signaling pathway for disulfide stress in Schizosaccharomyces pombe.
He Y et al. Nucleic Acids Res 2017 Jan 09;45(1):106-114
PMID:39473973 - Limiting 20S proteasome assembly leads to unbalanced nucleo-cytoplasmic distribution of 26S/30S proteasomes and chronic proteotoxicity.
Ruiz-Romero G et al. iScience 2024 Nov 15;27(11):111095
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:24768994 - The Schizosaccharomyces pombe Hikeshi/Opi10 protein has similar biochemical functions to its human homolog but acts in different physiological contexts.
Oda Y et al. FEBS Lett 2014 May 21;588(10):1899-905
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:17510629 - A genome-wide role for CHD remodelling factors and Nap1 in nucleosome disassembly.
Walfridsson J et al. EMBO J 2007 Jun 20;26(12):2868-79
PMID:31219728 - Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1 in the fission yeast Schizosaccharomyces pombe .
Cipakova I et al. Cell Cycle 2019 Jul;18(14):1532-1536
PMID:19756689 - SUMOylation is required for normal development of linear elements and wild-type meiotic recombination in Schizosaccharomyces pombe.
Spirek M et al. Chromosoma 2010 Feb;119(1):59-72
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:26152728 - A Two-step Protein Quality Control Pathway for a Misfolded DJ-1 Variant in Fission Yeast.
Mathiassen SG et al. J Biol Chem 2015 Aug 21;290(34):21141-21153
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:20231270 - Schizosaccharomyces pombe Dss1p is a DNA damage checkpoint protein that recruits Rad24p, Cdc25p, and Rae1p to DNA double-strand breaks.
Selvanathan SP et al. J Biol Chem 2010 May 07;285(19):14122-33
PMID:19581297 - Molecular chaperone Hsp70/Hsp90 prepares the mitochondrial outer membrane translocon receptor Tom71 for preprotein loading.
Li J et al. J Biol Chem 2009 Aug 28;284(35):23852-9
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:29866182 - RNAi-dependent heterochromatin assembly in fission yeast Schizosaccharomyces pombe requires heat-shock molecular chaperones Hsp90 and Mas5.
Okazaki K et al. Epigenetics Chromatin 2018 Jun 04;11(1):26
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:24146635 - Hsp70-Hsp40 chaperone complex functions in controlling polarized growth by repressing Hsf1-driven heat stress-associated transcription.
Vjestica A et al. PLoS Genet 2013;9(10):e1003886
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:33946513 - The Putative RNA-Binding Protein Dri1 Promotes the Loading of Kinesin-14/Klp2 to the Mitotic Spindle and Is Sequestered into Heat-Induced Protein Aggregates in Fission Yeast.
Yukawa M et al. Int J Mol Sci 2021 Apr 30;22(9)
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:33225241 - The Hsp40 Mas5 Connects Protein Quality Control and the General Stress Response through the Thermo-sensitive Pyp1.
Boronat S et al. iScience 2020 Nov 20;23(11):101725
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:24442611 - Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes.
Chen W et al. RNA 2014 Mar;20(3):308-20
PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.
Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:37446379 - Formation of Transient Protein Aggregate-like Centers Is a General Strategy Postponing Degradation of Misfolded Intermediates.
Boronat S et al. Int J Mol Sci 2023 Jul 07;24(13)
PMID:18794354 - Schizosaccharomyces pombe Ddb1 recruits substrate-specific adaptor proteins through a novel protein motif, the DDB-box.
Fukumoto Y et al. Mol Cell Biol 2008 Nov;28(22):6746-56
PMID:37615341 - Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1 .
Budden AM et al. Elife 2023 Aug 24;12
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:31294478 - Senataxin homologue Sen1 is required for efficient termination of RNA polymerase III transcription.
Rivosecchi J et al. EMBO J 2019 Aug 15;38(16):e101955
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
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PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
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PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
GO_REF:0000002 - Comments
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:24634168 - Proteome-wide search for PP2A substrates in fission yeast.
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PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
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PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
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PMID:23956636 - Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Proteome Analysis.
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PMID:32075773 - Chaperone-Facilitated Aggregation of Thermo-Sensitive Proteins Shields Them from Degradation during Heat Stress.
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PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
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PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
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