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protein coding gene - git3 (SPCC1753.02c) - G-protein coupled receptor Git3

Gene summary

Standard name
git3
Systematic ID
SPCC1753.02c
Product
G-protein coupled receptor Git3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94744
ORFeome ID
21/21F11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1542333..1546095 reverse strand

Annotation

GO biological process

GO:0010619 - adenylate cyclase-activating glucose-activated G protein-coupled receptor signaling pathway

References:

GO:0031138 - negative regulation of conjugation with cellular fusion

References:

GO cellular component

GO:0005886 - plasma membrane

References:

GO molecular function

GO:1990576 - G protein-coupled glucose receptor activity

References:

GO:0005085 - guanyl-nucleotide exchange factor activity

References:

GO:0005515 - protein binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0006993 - decreased chromatin silencing at centromere otr1R

References:

Genotypes:

FYPO:0005288 - decreased negative regulation of transcription by glucose

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0003032 - decreased RNA level during glucose starvation

References:

Genotypes:

FYPO:0003120 - decreased transcription during glucose starvation

References:

Genotypes:

FYPO:0001798 - decreased translation

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0003574 - normal histone H3-K14 acetylation at centromere outer repeat during vegetative growth

References:

Genotypes:

FYPO:0003235 - normal histone H3-K9 methylation at centromere outer repeat during vegetative growth

References:

Genotypes:

FYPO:0001865 - normal negative regulation of transcription by glucose

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0001317 - normal RNA level during vegetative growth

References:

Genotypes:

FYPO:0000098 - sensitive to calcium

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0000271 - sensitive to salt stress

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0000280 - sterile

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000240 - abnormal filament morphology

References:

Genotypes:

FYPO:0003625 - abnormal microtubule cytoskeleton morphology during mitotic interphase

References:

Genotypes:

FYPO:0000044 - abnormal negative regulation of transcription by glucose

References:

Genotypes:

FYPO:0000357 - abnormal shmoo morphology

References:

Genotypes:

FYPO:0007703 - abolished protein localization to lateral cell cortex during glucose starvation

References:

Genotypes:

FYPO:0002033 - abolished protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

References:

Genotypes:

FYPO:0001665 - decreased cellular cAMP level during cellular response to glucose stimulus

References:

Genotypes:

FYPO:0002143 - decreased cellular reactive oxygen species level in stationary phase

References:

Genotypes:

FYPO:0005288 - decreased negative regulation of transcription by glucose

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0000552 - decreased pseudohyphal growth

References:

Genotypes:

FYPO:0000582 - decreased rate of spore germination

References:

Genotypes:

FYPO:0001798 - decreased translation

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0009007 - decreased vegetative cell population viability

References:

Genotypes:

FYPO:0006012 - elongated cell with abolished shmoo formation

References:

Genotypes:

FYPO:0005258 - increased cell population growth at high temperature

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0009095 - increased cell population growth on fructose carbon source

References:

Genotypes:

FYPO:0006234 - increased cell population growth on gluconate carbon source

References:

Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0009011 - increased cell population viability on galactose carbon source

References:

Genotypes:

FYPO:0006979 - increased cellular coenzyme Q10 level

References:

Genotypes:

FYPO:0000565 - increased cellular respiration

References:

Genotypes:

FYPO:0006373 - increased histone H3-K9 dimethylation at heterochromatin island during vegetative growth

References:

Genotypes:

FYPO:0001043 - increased mating efficiency

References:

Genotypes:

FYPO:0004166 - increased oxygen consumption during vegetative growth

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0000780 - increased transcription during vegetative growth

References:

Genotypes:

FYPO:0004557 - increased vegetative cell population growth

References:

Genotypes:

FYPO:0009008 - increased vegetative cell population viability

References:

Genotypes:

FYPO:0001309 - increased viability in stationary phase

References:

Genotypes:

FYPO:0004163 - increased viability upon glucose starvation

References:

Genotypes:

FYPO:0004344 - increased viability upon nitrogen starvation

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Genotypes:

FYPO:0006013 - large ascus

References:

Genotypes:

FYPO:0001864 - mating without glucose starvation

References:

Genotypes:

FYPO:0004165 - normal glucose consumption

References:

Genotypes:

FYPO:0001865 - normal negative regulation of transcription by glucose

References:

Genotypes:

FYPO:0000776 - normal protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0000441 - resistance to antimycin A

References:

Genotypes:

FYPO:0000073 - resistance to caffeine

References:

Genotypes:

FYPO:0001884 - resistance to Calcofluor White

References:

Genotypes:

FYPO:0001029 - resistance to canavanine

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Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009047 - resistance to dimethyl sulfoxide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009034 - resistance to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0009035 - resistance to formamide

References:

Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

References:

Genotypes:

FYPO:0009070 - resistance to itraconazole

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0005969 - resistance to magnesium chloride

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001109 - resistance to menadione

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

References:

Genotypes:

FYPO:0009039 - resistance to potassium chloride

References:

Genotypes:

FYPO:0000077 - resistance to rapamycin

References:

Genotypes:

FYPO:0005266 - resistance to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002767 - resistance to terbinafine

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0005193 - resistance to torin1

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0001701 - sensitive to bortezomib

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000098 - sensitive to calcium

References:

Genotypes:

FYPO:0007926 - sensitive to calcofluor and potassium chloride

References:

Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0002640 - sensitive to clotrimazole

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0007932 - sensitive to hydroxyurea and methyl methanesulfonate

References:

Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000111 - sensitive to rapamycin

References:

Genotypes:

FYPO:0000271 - sensitive to salt stress

References:

Genotypes:

FYPO:0007925 - sensitive to sodium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0000112 - sensitive to sorbitol

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0002546 - sensitive to trichostatin A

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0006822 - viable small vegetative cell with normal cell growth rate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF11710Git3Glucose_rcpt_Git3-like_NPfam
PTHR23112PANTHER
Transmembrane alpha helixDeepTMHMM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:38598031 - Pps1, phosphatidylserine synthase, regulates the salt stress response in Schizosaccharomyces pombe.
Naozuka G et al. Mol Genet Genomics 2024 Apr 10;299(1):43
PMID:31030285 - CoQ 10 production in Schizosaccharomyces pombe is increased by reduction of glucose levels or deletion of pka1.
Nishida I et al. Appl Microbiol Biotechnol 2019 Jun;103(12):4899-4915
PMID:22252817 - A genomewide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis.
Fang Y et al. Antimicrob Agents Chemother 2012 Apr;56(4):1949-59
PMID:8001792 - Glucose repression of fbp1 transcription of Schizosaccharomyces pombe is partially regulated by adenylate cyclase activation by a G protein alpha subunit encoded by gpa2 (git8).
Nocero M et al. Genetics 1994 Sep;138(1):39-45
PMID:35171902 - The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly.
Bao K et al. PLoS Genet 2022 Feb;18(2):e1010049
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:1849107 - Glucose repression of transcription of the Schizosaccharomyces pombe fbp1 gene occurs by a cAMP signaling pathway.
Hoffman CS et al. Genes Dev 1991 Apr;5(4):561-71
PMID:7498728 - sck1, a high copy number suppressor of defects in the cAMP-dependent protein kinase pathway in fission yeast, encodes a protein homologous to the Saccharomyces cerevisiae SCH9 kinase.
Jin M et al. Genetics 1995 Jun;140(2):457-67
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:8227198 - Six git genes encode a glucose-induced adenylate cyclase activation pathway in the fission yeast Schizosaccharomyces pombe.
Byrne SM et al. J Cell Sci 1993 Aug;105 ( Pt 4)(0 4):1095-100
PMID:19266076 - Pro-aging effects of glucose signaling through a G protein-coupled glucose receptor in fission yeast.
Roux AE et al. PLoS Genet 2009 Mar;5(3):e1000408
PMID:2157626 - Isolation and characterization of mutants constitutive for expression of the fbp1 gene of Schizosaccharomyces pombe.
Hoffman CS et al. Genetics 1990 Apr;124(4):807-16
PMID:26443240 - PKA antagonizes CLASP-dependent microtubule stabilization to re-localize Pom1 and buffer cell size upon glucose limitation.
Kelkar M et al. Nat Commun 2015 Oct 07;6:8445
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:36112198 - Synergistic roles of the phospholipase B homolog Plb1 and the cAMP-dependent protein kinase Pka1 in the hypertonic stress response of Schizosaccharomyces pombe.
Matsuo Y et al. Curr Genet 2022 Dec;68(5-6):661-674
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:19542312 - Functional genomics of adhesion, invasion, and mycelial formation in Schizosaccharomyces pombe.
Dodgson J et al. Eukaryot Cell 2009 Aug;8(8):1298-306
PMID:24928510 - Glucose activates TORC2-Gad8 protein via positive regulation of the cAMP/cAMP-dependent protein kinase A (PKA) pathway and negative regulation of the Pmk1 protein-mitogen-activated protein kinase pathway.
Cohen A et al. J Biol Chem 2014 Aug 01;289(31):21727-37
PMID:10747045 - The fission yeast git5 gene encodes a Gbeta subunit required for glucose-triggered adenylate cyclase activation.
Landry S et al. Genetics 2000 Apr;154(4):1463-71
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
PMID:12715160 - The phospholipase B homolog Plb1 is a mediator of osmotic stress response and of nutrient-dependent repression of sexual differentiation in the fission yeast Schizosaccharomyces pombe.
Yang P et al. Mol Genet Genomics 2003 Apr;269(1):116-25
PMID:20536828 - A screen for genes involved in respiration control and longevity in Schizosaccharomyces pombe.
Roux AE et al. Ann N Y Acad Sci 2010 Jun;1197:19-27
PMID:24297439 - Sck1 negatively regulates Gpa2-mediated glucose signaling in Schizosaccharomyces pombe.
Mudge DK et al. Eukaryot Cell 2014 Feb;13(2):202-8
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16143612 - Schizosaccharomyces pombe adenylate cyclase suppressor mutations suggest a role for cAMP phosphodiesterase regulation in feedback control of glucose/cAMP signaling.
Wang L et al. Genetics 2005 Dec;171(4):1523-33
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23521895 - Chemical genetic screen in fission yeast reveals roles for vacuolar acidification, mitochondrial fission, and cellular GMP levels in lifespan extension.
Stephan J et al. Aging Cell 2013 Aug;12(4):574-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:8832414 - The Schizosaccharomyces pombe pyp1 protein tyrosine phosphatase negatively regulates nutrient monitoring pathways.
Santo PD et al. J Cell Sci 1996 Jul;109 ( Pt 7)(0 7):1919-1925
PMID:22624651 - A systematic screen reveals new elements acting at the G2/M cell cycle control.
Navarro FJ et al. Genome Biol 2012 May 24;13(5):R36
PMID:27756188 - cAMP-dependent protein kinase involves calcium tolerance through the regulation of Prz1 in Schizosaccharomyces pombe.
Matsuo Y et al. Biosci Biotechnol Biochem 2017 Feb;81(2):231-241
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:20139237 - Activated alleles of the Schizosaccharomyces pombe gpa2+ Galpha gene identify residues involved in GDP-GTP exchange.
Ivey FD et al. Eukaryot Cell 2010 Apr;9(4):626-33
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:25590601 - Fission yeast Ryh1 GTPase activates TOR Complex 2 in response to glucose.
Hatano T et al. Cell Cycle 2015;14(6):848-56
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:11014802 - Glucose monitoring in fission yeast via the Gpa2 galpha, the git5 Gbeta and the git3 putative glucose receptor.
Welton RM et al. Genetics 2000 Oct;156(2):513-21
PMID:25373780 - A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.
Graml V et al. Dev Cell 2014 Oct 27;31(2):227-239
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:34984977 - Functional profiling of long intergenic non-coding RNAs in fission yeast.
Rodriguez-Lopez M et al. Elife 2022 Jan 05;11
PMID:36358992 - Multi-Layered Regulations on the Chromatin Architectures: Establishing the Tight and Specific Responses of Fission Yeast fbp1 Gene Transcription.
Asada R et al. Biomolecules 2022 Nov 05;12(11)
PMID:38889144 - Cytoplasmic fluidization contributes to breaking spore dormancy in fission yeast.
Sakai K et al. Proc Natl Acad Sci U S A 2024 Jun 25;121(26):e2405553121