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protein coding gene - bgs4 (SPCC1840.02c) - cell wall and secondary septum 1,6 branched 1,3-beta-glucan synthase catalytic subunit Bgs4

Gene summary

Standard name
bgs4
Systematic ID
SPCC1840.02c
Product
cell wall and secondary septum 1,6 branched 1,3-beta-glucan synthase catalytic subunit Bgs4
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
orb11, cwg1, pbr1, sph1
UniProt ID
O74475
ORFeome ID
34/34B11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 2248859..2256652 reverse strand

Annotation

PBO:0001859 - 2.4.1.34

GO biological process

GO:0071970 - fungal-type cell wall (1->3)-beta-D-glucan biosynthetic process

References:

GO:1990344 - secondary cell septum biogenesis

References:

GO cellular component

GO:0000148 - 1,3-beta-D-glucan synthase complex

References:

GO:1902716 - cell cortex of growing cell tip

References:

GO:0032153 - cell division site

References:

GO:0051286 - cell tip

References:

GO:0032154 - cleavage furrow

References:

GO:0005737 - cytoplasm

References:

GO:0000935 - division septum

References:

GO:0005794 - Golgi apparatus

References:

GO:0043332 - mating projection tip

References:

GO:0110085 - mitotic actomyosin contractile ring

References:

GO:0035841 - new growing cell tip

References:

GO:0031520 - plasma membrane of cell tip

References:

GO:0000936 - primary cell septum

References:

GO:0030427 - site of polarized growth

References:

GO:0031160 - spore wall

References:

GO molecular function

GO:0003843 - 1,3-beta-D-glucan synthase activity

References:

GO:0140752 - branched 1,3-beta-D-glucan synthase activity

References:

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0004481 - abolished cell population growth at high temperature

References:

Genotypes:

FYPO:0000583 - abolished sporulation

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0001235 - decreased extent of cell population growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000647 - vegetative cell lysis

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000005 - abnormal cell morphology

References:

Genotypes:

FYPO:0000164 - abnormal cell separation after cytokinesis

References:

Genotypes:

FYPO:0001418 - abnormal microtubule cytoskeleton morphology during vegetative growth

References:

Genotypes:

FYPO:0001118 - abnormal vegetative cell morphology

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Genotypes:

FYPO:0000417 - abolished cytokinesis

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Genotypes:

FYPO:0003202 - actomyosin contractile ring contraction uncoupled from septum assembly

References:

Genotypes:

FYPO:0002027 - angled actomyosin contractile ring

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Genotypes:

FYPO:0003203 - curved septum

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0001082 - decreased cell wall beta-glucan level

References:

Genotypes:

FYPO:0002265 - decreased cell wall galactomannan level

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Genotypes:

FYPO:0003889 - decreased cell wall thickness at old end during vegetative growth

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Genotypes:

FYPO:0001088 - decreased glucosyltransferase activity

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Genotypes:

FYPO:0003205 - decreased primary cell septum thickness

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Genotypes:

FYPO:0001365 - decreased rate of actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0003201 - decreased rate of primary cell septum biogenesis

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002021 - dispersed actin cortical patch localization during vegetative growth

References:

Genotypes:

FYPO:0001122 - elongated vegetative cell

References:

Genotypes:

FYPO:0001970 - increased 1,3-beta-D-glucan synthase activity during growth on aculeacin A

References:

Genotypes:

FYPO:0001969 - increased 1,3-beta-D-glucan synthase activity during growth on papulacandin B

References:

Genotypes:

FYPO:0001084 - increased cell wall alpha-glucan level

References:

Genotypes:

FYPO:0002264 - increased concentration of polysaccharide in growth medium

References:

Genotypes:

FYPO:0001406 - increased septum thickness

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000224 - lemon-shaped cell

References:

Genotypes:

FYPO:0001390 - misoriented septum during vegetative growth

References:

Genotypes:

FYPO:0004893 - normal 1,3-beta-D-glucan synthase activity during vegetative growth

References:

Genotypes:

FYPO:0001367 - normal cytokinesis

References:

Genotypes:

FYPO:0001086 - normal glucosyltransferase activity

References:

Genotypes:

FYPO:0001192 - normal growth on cell wall-degrading enzymes

References:

Genotypes:

FYPO:0002442 - normal protein localization to cell division site

References:

Genotypes:

FYPO:0008003 - normal protein localization to site of mechanical stress

References:

Genotypes:

FYPO:0002085 - normal vegetative cell growth

References:

Genotypes:

FYPO:0001315 - normal vegetative cell morphology

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0001120 - pear-shaped vegetative cell

References:

Genotypes:

FYPO:0001964 - resistance to aculeacin A

References:

Genotypes:

FYPO:0005152 - resistance to caspofungin

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0005150 - resistance to enfumafungin

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001963 - resistance to papulacandin B

References:

Genotypes:

FYPO:0001965 - resistance to papulacandin D

References:

Genotypes:

FYPO:0005151 - resistance to pneumocandin B0

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0003204 - secondary cell septum absent from cell during vegetative growth

References:

Genotypes:

FYPO:0001966 - sensitive to aculeacin A

References:

Genotypes:

FYPO:0001190 - sensitive to cell wall-degrading enzymes

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0005149 - septated spheroid vegetative cell

References:

Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

FYPO:0000024 - stubby vegetative cell

References:

Genotypes:

FYPO:0000646 - swollen vegetative cell

References:

Genotypes:

FYPO:0000647 - vegetative cell lysis

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0004103 - viable spherical vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

Protein features

IDNameInterPro nameDB name
PF02364Glucan_synthaseGlyco_trans_48_domPFAM
PF23605FKS1_dom2FKS1-like_dom2PFAM
PF14288FKS1_dom1FKS1-like_dom1PFAM
SM01205FKS1_dom1_2FKS1-like_dom1SMART
PTHR12741LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1PANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMOBIDB-Positive-Polyelectrolyte

Orthologs

References / Literature

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:26132084 - Cooperation between Paxillin-like Protein Pxl1 and Glucan Synthase Bgs1 Is Essential for Actomyosin Ring Stability and Septum Formation in Fission Yeast.
Cortés JC et al. PLoS Genet 2015 Jul;11(7):e1005358
GO_REF:0000044 - Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.
PMID:16421249 - Rgf1p is a specific Rho1-GEF that coordinates cell polarization with cell wall biogenesis in fission yeast.
García P et al. Mol Biol Cell 2006 Apr;17(4):1620-31
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.
Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:20624220 - Kin1 is a plasma membrane-associated kinase that regulates the cell surface in fission yeast.
Cadou A et al. Mol Microbiol 2010 Sep;77(5):1186-202
PMID:41259369 - Hydroxyurea induces an oxidative stress response that triggers ER expansion and cytoplasmic protein aggregation.
Sánchez-Molina A et al. PLoS Biol 2025 Nov 19;23(11):e3003493
GO_REF:0000051 - S. pombe keyword mapping
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:27974503 - Traffic Through the Trans-Golgi Network and the Endosomal System Requires Collaboration Between Exomer and Clathrin Adaptors in Fission Yeast.
Hoya M et al. Genetics 2017 Feb;205(2):673-690
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:26749213 - The AP-2 complex is required for proper temporal and spatial dynamics of endocytic patches in fission yeast.
de León N et al. Mol Microbiol 2016 May;100(3):409-24
PMID:27168121 - Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. Yeast 2016 Sep;33(9):507-17
PMID:25977474 - A role for F-BAR protein Rga7p during cytokinesis in S. pombe.
Arasada R et al. J Cell Sci 2015 Jul 01;128(13):2259-68
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.
Malecki M et al. Genome Biol 2016 Nov 25;17(1):240
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:27385337 - Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis.
Liu Y et al. Mol Biol Cell 2016 Aug 15;27(16):2528-41
PMID:25825517 - A formin-nucleated actin aster concentrates cell wall hydrolases for cell fusion in fission yeast.
Dudin O et al. J Cell Biol 2015 Mar 30;208(7):897-911
PMID:28945192 - Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe .
Guydosh NR et al. Elife 2017 Sep 25;6
PMID:37792890 - Elevated levels of sphingolipid MIPC in the plasma membrane disrupt the coordination of cell growth with cell wall formation in fission yeast.
Willet AH et al. PLoS Genet 2023 Oct;19(10):e1010987
PMID:20870879 - Reorganization of the growth pattern of Schizosaccharomyces pombe in invasive filament formation.
Dodgson J et al. Eukaryot Cell 2010 Nov;9(11):1788-97
PMID:21115488 - Differential activities of three families of specific beta(1,3)glucan synthase inhibitors in wild-type and resistant strains of fission yeast.
Martins IM et al. J Biol Chem 2011 Feb 04;286(5):3484-96
PMID:15615781 - The novel fission yeast (1,3)beta-D-glucan synthase catalytic subunit Bgs4p is essential during both cytokinesis and polarized growth.
Cortés JC et al. J Cell Sci 2005 Jan 01;118(Pt 1):157-74
PMID:2127797 - Glycoprotein molecules in the walls of Schizosaccharomyces pombe wild-type cells and a morphologically altered mutant resistant to papulacandin B.
Font de Mora J et al. J Gen Microbiol 1990 Nov;136(11):2251-9
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:30332655 - Paxillin-Mediated Recruitment of Calcineurin to the Contractile Ring Is Required for the Correct Progression of Cytokinesis in Fission Yeast.
Martín-García R et al. Cell Rep 2018 Oct 16;25(3):772-783.e4
PMID:26941334 - Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis.
Wei B et al. Mol Biol Cell 2016 Apr 15;27(8):1235-45
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:23977061 - Regulation of cell wall synthesis by the clathrin light chain is essential for viability in Schizosaccharomyces pombe.
de León N et al. PLoS One 2013;8(8):e71510
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:26702831 - Oligomerization but Not Membrane Bending Underlies the Function of Certain F-BAR Proteins in Cell Motility and Cytokinesis.
McDonald NA et al. Dev Cell 2015 Dec 21;35(6):725-36
PMID:7592316 - Papulacandin B resistance in budding and fission yeasts: isolation and characterization of a gene involved in (1,3)beta-D-glucan synthesis in Saccharomyces cerevisiae.
Castro C et al. J Bacteriol 1995 Oct;177(20):5732-9
PMID:25422470 - Oscillatory AAA+ ATPase Knk1 constitutes a novel morphogenetic pathway in fission yeast.
Scheffler K et al. Proc Natl Acad Sci U S A 2014 Dec 16;111(50):17899-904
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:1828464 - Isolation and characterization of Schizosaccharomyces pombe mutants defective in cell wall (1-3)beta-D-glucan.
Ribas JC et al. J Bacteriol 1991 Jun;173(11):3456-62
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
Morozumi Y et al. iScience 2024 Jan 19;27(1):108777
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:24165938 - Extracellular cell wall β(1,3)glucan is required to couple septation to actomyosin ring contraction.
Muñoz J et al. J Cell Biol 2013 Oct 28;203(2):265-82
PMID:39156640 - Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic.
Ramos M et al. iScience 2024 Aug 16;27(8):110477
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:40668835 - α-glucan remodeling by GH13-domain enzymes shapes fungal cell wall architecture.
Jacob A et al. Proc Natl Acad Sci U S A 2025 Jul 22;122(29):e2505509122
PMID:29689193 - Mechanosensation Dynamically Coordinates Polar Growth and Cell Wall Assembly to Promote Cell Survival.
Davì V et al. Dev Cell 2018 Apr 23;45(2):170-182.e7
PMID:29930085 - Local and global Cdc42 guanine nucleotide exchange factors for fission yeast cell polarity are coordinated by microtubules and the Tea1-Tea4-Pom1 axis.
Tay YD et al. J Cell Sci 2018 Jul 19;131(14)
PMID:34910579 - Involvement of Smi1 in cell wall integrity and glucan synthase Bgs4 localization during fission yeast cytokinesis.
Longo LVG et al. Mol Biol Cell 2022 Feb 01;33(2):ar17
PMID:21148300 - Actin cables and the exocyst form two independent morphogenesis pathways in the fission yeast.
Bendezú FO et al. Mol Biol Cell 2011 Jan 01;22(1):44-53
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:34666001 - Detection of surface forces by the cell-wall mechanosensor Wsc1 in yeast.
Neeli-Venkata R et al. Dev Cell 2021 Oct 25;56(20):2856-2870.e7
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:31116668 - Fission yeast TRP channel Pkd2p localizes to the cleavage furrow and regulates cell separation during cytokinesis.
Morris Z et al. Mol Biol Cell 2019 Jul 15;30(15):1791-1804
PMID:20603077 - Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site.
Roberts-Galbraith RH et al. Mol Cell 2010 Jul 09;39(1):86-99
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:34959732 - Echinocandin Drugs Induce Differential Effects in Cytokinesis Progression and Cell Integrity.
Yagüe N et al. Pharmaceuticals (Basel) 2021 Dec 20;14(12)
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:11069657 - bgs2+, a sporulation-specific glucan synthase homologue is required for proper ascospore wall maturation in fission yeast.
Martín V et al. Mol Microbiol 2000 Oct;38(2):308-21
PMID:30044717 - Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis.
Zhu YH et al. Mol Biol Cell 2018 Sep 15;29(19):2259-2279
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:41144523 - The Ync13-Rga7-Rng10 complex selectively coordinates secretory vesicle trafficking and secondary septum formation during cytokinesis.
Zhang S et al. PLoS Biol 2025 Oct 27;23(10):e3003466
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:8522609 - Fission yeast cell morphogenesis: identification of new genes and analysis of their role during the cell cycle.
Verde F et al. J Cell Biol 1995 Dec;131(6 Pt 1):1529-38