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protein coding gene - spc25 (SPCC188.04c) - Ndc80 complex subunit Spc25

Gene summary

Standard name
spc25
Systematic ID
SPCC188.04c
Product
Ndc80 complex subunit Spc25
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q10430
ORFeome ID
51/51C02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1478061..1481966 reverse strand

Annotation

GO biological process

GO:1990571 - meiotic centromere clustering

References:

GO:0051455 - spindle attachment to meiosis I kinetochore

References:

GO cellular component

GO:0000779 - condensed chromosome, centromeric region

References:

GO:0000776 - kinetochore

References:

GO:0031262 - Ndc80 complex

References:

GO:0005634 - nucleus

References:

GO:0000940 - outer kinetochore

References:

GO molecular function

GO:0008017 - microtubule binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF08234Spindle_Spc25Spc25_CPfam
PF29997SPC25_NPfam
cd23784RWD_Spc25CDD
G3DSA:3.30.457.50:FF:000001CATH-FunFam
G3DSA:3.30.457.50CATH-Gene3D
PTHR14281Spc25PANTHER
CoilCOILS

Orthologs

References / Literature

PMID:24477934 - Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores.
Heinrich S et al. EMBO Rep 2014 Mar;15(3):291-8
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:28502666 - Purification and characterisation of the fission yeast Ndc80 complex.
Matsuo Y et al. Protein Expr Purif 2017 Jul;135:61-69
PMID:26670050 - Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1.
Kilchert C et al. Cell Rep 2015 Dec 22;13(11):2504-2515
PMID:15371542 - The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association.
Kerres A et al. Mol Biol Cell 2004 Dec;15(12):5255-67
PMID:16079914 - Molecular analysis of kinetochore architecture in fission yeast.
Liu X et al. EMBO J 2005 Aug 17;24(16):2919-30
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:15728720 - Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeast.
Asakawa H et al. Mol Biol Cell 2005 May;16(5):2325-38
PMID:17035632 - Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe.
Hayashi A et al. Mol Biol Cell 2006 Dec;17(12):5173-84
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:23427262 - The internal loop of fission yeast Ndc80 binds Alp7/TACC-Alp14/TOG and ensures proper chromosome attachment.
Tang NH et al. Mol Biol Cell 2013 Apr;24(8):1122-33
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:36705602 - Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging.
Virant D et al. J Cell Biol 2023 Apr 03;222(4)
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053