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protein coding gene - srp54 (SPCC188.06c) - signal recognition particle subunit Srp54

Gene summary

Standard name
srp54
Systematic ID
SPCC188.06c
Product
signal recognition particle subunit Srp54
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P21565
ORFeome ID
51/51E05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1482990..1485200 reverse strand

Annotation

Complementation

PBO:0016115 - is not functionally complemented by S. cerevisiae SRP54

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Disease association

MONDO:0032899 - neutropenia, severe congenital, 8, autosomal dominant

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GO biological process

GO:0006614 - SRP-dependent cotranslational protein targeting to membrane

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GO:0006616 - SRP-dependent cotranslational protein targeting to membrane, translocation

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GO cellular component

GO:0005829 - cytosol

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GO:0005783 - endoplasmic reticulum

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GO:0005786 - signal recognition particle, endoplasmic reticulum targeting

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GO molecular function

GO:0008312 - 7S RNA binding

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GO:0030942 - endoplasmic reticulum signal sequence receptor activity

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GO:0005525 - GTP binding

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GO:0003924 - GTPase activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0000081 - sensitive to high osmolarity

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0001531 - decreased GTPase activity

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Genotypes:

FYPO:0003447 - decreased level of glycosylated protein in cell

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Genotypes:

FYPO:0003446 - decreased SRP-dependent cotranslational protein targeting to membrane during vegetative growth

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Genotypes:

FYPO:0001042 - inviable after spore germination, single or double cell division

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

FYPO:0000021 - spheroid vegetative cell

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Genotypes:

FYPO:0003448 - viable swollen vacuolated spheroid vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00448SRP54SRP54_GTPase_domPFAM
PF02881SRP54_NSignal_recog_particl_SRP54_hlxPFAM
PF02978SRP_SPBSignal_recog_particle_SRP54_MPFAM
cd17875SRP54_GCDD
PS00300SRP54SRP54_GTPase_domPROSITE_PATTERNS
SM00962SRP54_3SRP54_GTPase_domSMART
SM00382AAA_5AAA+_ATPaseSMART
SM00963SRP54_N_2Signal_recog_particl_SRP54_hlxSMART
G3DSA:3.40.50.300:FF:000022FUNFAM
G3DSA:1.10.260.30:FF:000003FUNFAM
G3DSA:1.20.120.140:FF:000001FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
SSF47446Signal peptide-binding domainSignal_recog_part_SRP54_M_sfSUPERFAMILY
G3DSA:1.20.120.140SRP54_N_sfGENE3D
G3DSA:1.10.260.30Signal_recog_part_SRP54_M_sfGENE3D
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
PTHR11564SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54SRP54PANTHER
MF_00306SRP54SRP54HAMAP
TIGR01425SRP54_eukSRP54_eukNCBIFAM

Orthologs

References / Literature

GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:8382769 - Identification of RNA sequences and structural elements required for assembly of fission yeast SRP54 protein with signal recognition particle RNA.
Selinger D et al. Mol Cell Biol 1993 Mar;13(3):1353-62
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:8041618 - Genetic and biochemical analysis of the fission yeast ribonucleoprotein particle containing a homolog of Srp54p.
Selinger D et al. Nucleic Acids Res 1994 Jul 11;22(13):2557-67
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:19682301 - A large complex mediated by Moc1, Moc2 and Cpc2 regulates sexual differentiation in fission yeast.
Paul SK et al. FEBS J 2009 Sep;276(18):5076-93
PMID:10079327 - Systematic mutagenesis of the fission yeast Srp54 protein.
Martínez-Force E et al. Curr Genet 1999 Mar;35(2):88-102
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:7969124 - The Srp54 GTPase is essential for protein export in the fission yeast Schizosaccharomyces pombe.
Althoff SM et al. Mol Cell Biol 1994 Dec;14(12):7839-54
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000002 - Comments
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:2557350 - Saccharomyces cerevisiae and Schizosaccharomyces pombe contain a homologue to the 54-kD subunit of the signal recognition particle that in S. cerevisiae is essential for growth.
Hann BC et al. J Cell Biol 1989 Dec;109(6 Pt 2):3223-30
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)