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protein coding gene - dom34 (SPCC18B5.06) - Dom34-Hbs1 translation release factor complex subunit, peloto ortholog Dom34

Gene summary

Standard name
dom34
Systematic ID
SPCC18B5.06
Product
Dom34-Hbs1 translation release factor complex subunit, peloto ortholog Dom34
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
erf1, sup45
UniProt ID
Q9USL5
ORFeome ID
44/44D11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 729108..730829 forward strand

Annotation

GO biological process

GO:0002184 - cytoplasmic translational termination

References:

GO:0030968 - endoplasmic reticulum unfolded protein response

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GO:0070966 - nuclear-transcribed mRNA catabolic process, no-go decay

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GO:0070481 - nuclear-transcribed mRNA catabolic process, non-stop decay

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GO:0072344 - rescue of stalled cytosolic ribosome

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GO:0071025 - RNA surveillance

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GO cellular component

GO:0005737 - cytoplasm

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GO:0005829 - cytosol

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GO:0022626 - cytosolic ribosome

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GO:1990533 - Dom34-Hbs1 complex

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GO:0005634 - nucleus

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GO molecular function

GO:0030695 - GTPase regulator activity

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GO:0170011 - stalled ribosome sensor activity

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GO:0003747 - translation release factor activity

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Multi-locus phenotype

FYPO:0006255 - increased level of short ribosome footprints during endoplasmic reticulum unfolded protein response

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Genotypes:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000121 - abnormal sporulation

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Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0009052 - increased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0009030 - resistance to amitrole

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Genotypes:

FYPO:0009036 - resistance to benzamidine

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Genotypes:

FYPO:0009031 - resistance to bleomycin

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009040 - resistance to tea tree oil

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Genotypes:

FYPO:0002767 - resistance to terbinafine

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Genotypes:

FYPO:0001098 - sensitive to 4-nitroquinoline N-oxide

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Genotypes:

FYPO:0007921 - sensitive to benzamidine

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Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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FYPO:0009071 - sensitive to itraconazole

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FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0005889 - sensitive to sodium chloride

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF03464eRF1_2eRF1_2Pfam
PF03465eRF1_3eRF1_3Pfam
PF26356Pelota_NPelota_NPfam
SM01194eRF1_1eRF1_Pelota-like_NSMART
G3DSA:2.30.30.870:FF:000001CATH-FunFam
G3DSA:3.30.1330.30:FF:000008CATH-FunFam
G3DSA:3.30.420.60:FF:000004CATH-FunFam
G3DSA:2.30.30.870Pelota/DOM34_NCATH-Gene3D
G3DSA:3.30.1330.30Ribosomal_eL30-like_sfCATH-Gene3D
G3DSA:3.30.420.60eFR1_2_sfCATH-Gene3D
SSF159065Pelota/DOM34_NSUPERFAMILY
SSF53137SUPERFAMILY
SSF55315Ribosomal_eL30-like_sfSUPERFAMILY
PTHR10853TF_pelotaPANTHER
TIGR00111pelotaTF_pelotaNCBIFAM

Orthologs

References / Literature

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:15548596 - Analysis of mutant phenotypes and splicing defects demonstrates functional collaboration between the large and small subunits of the essential splicing factor U2AF in vivo.
Webb CJ et al. Mol Biol Cell 2005 Feb;16(2):584-96
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
GO_REF:0000002 - Comments
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:28945192 - Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe .
Guydosh NR et al. Elife 2017 Sep 25;6
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:9701287 - The stretch of C-terminal acidic amino acids of translational release factor eRF1 is a primary binding site for eRF3 of fission yeast.
Ito K et al. RNA 1998 Aug;4(8):958-72
PMID:10376874 - C-terminal interaction of translational release factors eRF1 and eRF3 of fission yeast: G-domain uncoupled binding and the role of conserved amino acids.
Ebihara K et al. RNA 1999 Jun;5(6):739-50
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:28947618 - Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.
Thakran P et al. EMBO J 2018 Jan 04;37(1):89-101
PMID:15576681 - Genetics of lagging strand DNA synthesis and maturation in fission yeast: suppression analysis links the Dna2-Cdc24 complex to DNA polymerase delta.
Tanaka H et al. Nucleic Acids Res 2004;32(21):6367-77
PMID:20890290 - Structure of the Dom34-Hbs1 complex and implications for no-go decay.
Chen L et al. Nat Struct Mol Biol 2010 Oct;17(10):1233-40