protein coding gene - gad8 (SPCC24B10.07) - serine/threonine protein kinase (AGC family) Gad8

Gene summary

Standard name

gad8

Systematic ID

SPCC24B10.07

Product

serine/threonine protein kinase (AGC family) Gad8

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

Q9P7J8

ORFeome ID

45/45G11

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome III: 912500..915238 forward strand

Annotation

Protein features

Orthologs

• SGK1 (HGNC:10810) Homo sapiens
• SGK3 (HGNC:10812) Homo sapiens
• SGK2 (HGNC:13900) Homo sapiens
• AKT1 (HGNC:391) Homo sapiens
• AKT2 (HGNC:392) Homo sapiens
• gad8 (SJAG_04206) Schizosaccharomyces japonicus
• YPK1 (YKL126W) Saccharomyces cerevisiae
• YPK2 (YMR104C) Saccharomyces cerevisiae

References / Literature

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:28041796 - A PP2A-B55-Mediated Crosstalk between TORC1 and TORC2 Regulates the Differentiation Response in Fission Yeast.

Martín R et al. Curr Biol 2017 Jan 23;27(2):175-188

PMID:27876895 - Multiple crosstalk between TOR and the cell integrity MAPK signaling pathway in fission yeast.

Madrid M et al. Sci Rep 2016 Nov 23;6:37515

PMID:31477575 - Modulation of TOR complex 2 signaling by the stress-activated MAPK pathway in fission yeast.

Morigasaki S et al. J Cell Sci 2019 Oct 10;132(19)

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:22976295 - Psk1, an AGC kinase family member in fission yeast, is directly phosphorylated and controlled by TORC1 and functions as S6 kinase.

Nakashima A et al. J Cell Sci 2012 Dec 01;125(Pt 23):5840-9

PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.

Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480

PMID:29084823 - Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast.

Navarro FJ et al. J Cell Sci 2017 Dec 15;130(24):4144-4154

GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:28264193 - Substrate specificity of TOR complex 2 is determined by a ubiquitin-fold domain of the Sin1 subunit.

Tatebe H et al. Elife 2017 Mar 07;6

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:24344203 - Isp7 is a novel regulator of amino acid uptake in the TOR signaling pathway.

Laor D et al. Mol Cell Biol 2014 Mar;34(5):794-806

PMID:23213482 - TORC2 and the AGC kinase Gad8 regulate phosphorylation of the ribosomal protein S6 in fission yeast.

Du W et al. Biol Open 2012 Sep 15;1(9):884-8

PMID:28476936 - Genome-wide screen for cell growth regulators in fission yeast.

Weston L et al. J Cell Sci 2017 Jun 15;130(12):2049-2055

PMID:31315658 - Leo1 is essential for the dynamic regulation of heterochromatin and gene expression during cellular quiescence.

Oya E et al. Epigenetics Chromatin 2019 Jul 17;12(1):45

PMID:35157728 - TOR complex 2 contributes to regulation of gene expression via inhibiting Gcn5 recruitment to subtelomeric and DNA replication stress genes.

Cohen A et al. PLoS Genet 2022 Feb;18(2):e1010061

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:27191590 - Fission Yeast SCYL1/2 Homologue Ppk32: A Novel Regulator of TOR Signalling That Governs Survival during Brefeldin A Induced Stress to Protein Trafficking.

Kowalczyk KM et al. PLoS Genet 2016 May;12(5):e1006041

PMID:19546237 - TOR complex 2 controls gene silencing, telomere length maintenance, and survival under DNA-damaging conditions.

Schonbrun M et al. Mol Cell Biol 2009 Aug;29(16):4584-94

PMID:19417002 - Fission yeast Tor1 functions as part of TORC1 to control mitotic entry through the stress MAPK pathway following nutrient stress.

Hartmuth S et al. J Cell Sci 2009 Jun 01;122(Pt 11):1737-46

PMID:23551936 - TORC1 signaling inhibition by rapamycin and caffeine affect lifespan, global gene expression, and cell proliferation of fission yeast.

Rallis C et al. Aging Cell 2013 Aug;12(4):563-73

PMID:18235227 - Fission yeast TOR complex 2 activates the AGC-family Gad8 kinase essential for stress resistance and cell cycle control.

Ikeda K et al. Cell Cycle 2008 Feb 01;7(3):358-64

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:23703609 - TORC2 is required to maintain genome stability during S phase in fission yeast.

Schonbrun M et al. J Biol Chem 2013 Jul 05;288(27):19649-60

PMID:29699848 - Phosphorylation of Wat1, human Lst8 homolog is critical for the regulation of TORC2 -Gad8 dependent pathway in fission yeast Schizosacchromyces pombe.

Ahamad N et al. Eur J Cell Biol 2018 May;97(4):300-307

PMID:26152587 - TORC1 Regulates Developmental Responses to Nitrogen Stress via Regulation of the GATA Transcription Factor Gaf1.

Laor D et al. mBio 2015 Jul 07;6(4):e00959

PMID:32361273 - Requirement of PP2A-B56 Par1 for the Stabilization of the CDK Inhibitor Rum1 and Activation of APC/C Ste9 during Pre-Start G1 in S. pombe.

Stonyte V et al. iScience 2020 May 22;23(5):101063

PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.

Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:26935949 - Phosphorylation of the amino-terminus of the AGC kinase Gad8 prevents its interaction with TORC2.

Du W et al. Open Biol 2016 Mar;6(3)

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:41258116 - TORC2 inactivation promotes heterochromatin formation in rDNA and prolongs viability of quiescent fission yeast cells.

Hirai H et al. Commun Biol 2025 Nov 19;8(1):1606

PMID:23271606 - Fission yeast TOR signaling is essential for the down-regulation of a hyperactivated stress-response MAP kinase under salt stress.

Ishiguro J et al. Mol Genet Genomics 2013 Feb;288(1-2):63-75

PMID:34028542 - TORC2 inhibition of α-arrestin Aly3 mediates cell surface persistence of S. pombe Ght5 glucose transporter in low glucose.

Toyoda Y et al. J Cell Sci 2021 May 15;134(10)

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:20501954 - Search for kinases related to transition of growth polarity in fission yeast.

Koyano T et al. Biosci Biotechnol Biochem 2010;74(5):1129-33

PMID:15821139 - Systematic deletion analysis of fission yeast protein kinases.

Bimbó A et al. Eukaryot Cell 2005 Apr;4(4):799-813

PMID:33137119 - TOR Complex 2- independent mutations in the regulatory PIF pocket of Gad8AKT1/SGK1 define separate branches of the stress response mechanisms in fission yeast.

Pataki E et al. PLoS Genet 2020 Nov;16(11):e1009196

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:25590601 - Fission yeast Ryh1 GTPase activates TOR Complex 2 in response to glucose.

Hatano T et al. Cell Cycle 2015;14(6):848-56

PMID:29079657 - TORC1 and TORC2 converge to regulate the SAGA co-activator in response to nutrient availability.

Laboucarié T et al. EMBO Rep 2017 Dec;18(12):2197-2218

PMID:26912660 - Gad8 Protein Is Found in the Nucleus Where It Interacts with the MluI Cell Cycle Box-binding Factor (MBF) Transcriptional Complex to Regulate the Response to DNA Replication Stress.

Cohen A et al. J Biol Chem 2016 Apr 22;291(17):9371-81

PMID:12805221 - Schizosaccharomyces pombe AGC family kinase Gad8p forms a conserved signaling module with TOR and PDK1-like kinases.

Matsuo T et al. EMBO J 2003 Jun 16;22(12):3073-83

PMID:28643116 - Express yourself: how PP2A-B55 Pab1 helps TORC1 talk to TORC2.

Martín R et al. Curr Genet 2018 Feb;64(1):43-51

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:17261596 - Loss of the TOR kinase Tor2 mimics nitrogen starvation and activates the sexual development pathway in fission yeast.

Matsuo T et al. Mol Cell Biol 2007 Apr;27(8):3154-64

PMID:25411338 - Mechanisms of expression and translocation of major fission yeast glucose transporters regulated by CaMKK/phosphatases, nuclear shuttling, and TOR.

Saitoh S et al. Mol Biol Cell 2015 Jan 15;26(2):373-86

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:23370392 - A knockout screen for protein kinases required for the proper meiotic segregation of chromosomes in the fission yeast Schizosaccharomyces pombe.

Kovacikova I et al. Cell Cycle 2013 Feb 15;12(4):618-24

PMID:29632066 - TOR complex 2 in fission yeast is required for chromatin-mediated gene silencing and assembly of heterochromatic domains at subtelomeres.

Cohen A et al. J Biol Chem 2018 May 25;293(21):8138-8150

PMID:24928510 - Glucose activates TORC2-Gad8 protein via positive regulation of the cAMP/cAMP-dependent protein kinase A (PKA) pathway and negative regulation of the Pmk1 protein-mitogen-activated protein kinase pathway.

Cohen A et al. J Biol Chem 2014 Aug 01;289(31):21727-37

PMID:38971312 - TORC2 is required for accumulation of γH2A in response to DNA damage.

Cohen A et al. J Biol Chem 2024 Jul 04;:107531

PMID:21035342 - Rab-family GTPase regulates TOR complex 2 signaling in fission yeast.

Tatebe H et al. Curr Biol 2010 Nov 23;20(22):1975-82

PMID:35924983 - Genetic-interaction screens uncover novel biological roles and regulators of transcription factors in fission yeast.

Chatfield-Reed K et al. G3 (Bethesda) 2022 Aug 25;12(9)

PMID:30680437 - Down-regulation of Cdk1 activity in G1 coordinates the G1/S gene expression programme with genome replication.

García-Blanco N et al. Curr Genet 2019 Jun;65(3):685-690

PMID:34686329 - The Hsp90 cochaperone TTT promotes cotranslational maturation of PIKKs prior to complex assembly.

Toullec D et al. Cell Rep 2021 Oct 19;37(3):109867

PMID:27206859 - TOR complex 2 localises to the cytokinetic actomyosin ring and controls the fidelity of cytokinesis.

Baker K et al. J Cell Sci 2016 Jul 01;129(13):2613-24

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:24247430 - Phosphorylation of the TOR ATP binding domain by AGC kinase constitutes a novel mode of TOR inhibition.

Hálová L et al. J Cell Biol 2013 Nov 25;203(4):595-604