protein coding gene - pho843 (SPCC2H8.02) - plasma membrane inorganic phosphate transmembrane transporter Pho843

Gene summary

Standard name: pho843
Systematic ID: SPCC2H8.02
Product: plasma membrane inorganic phosphate transmembrane transporter Pho843
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: Q9Y7Q9
ORFeome ID: 36/36A09
Characterisation status: biological role published
Feature type: mRNA gene
Genomic location: chromosome III: 827971..830947 forward strand

Annotation

GO biological process

GO:0035435 - phosphate ion transmembrane transport

References:

GO_REF:0000024

GO cellular component

GO:0005789 - endoplasmic reticulum membrane

References:

PMID:16823372

GO:0005794 - Golgi apparatus

References:

PMID:16823372

GO:0000139 - Golgi membrane

References:

PMID:16823372

GO:0005886 - plasma membrane

References:

GO_REF:0000033

GO molecular function

GO:0005315 - phosphate transmembrane transporter activity

References:

GO_REF:0000024

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:01148 - ubiquitinylated lysine

References:

PMID:37970674

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

PMID:11152613

Qualitative gene expression

PomGeneEx:0000027 - ribosomal density decreased

References:

PMID:33313903

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0009031 - resistance to bleomycin

References:

PMID:37787768

Genotypes:

pho843delta

FYPO:0000067 - resistance to brefeldin A

References:

PMID:37787768

Genotypes:

pho843delta

FYPO:0002693 - resistance to diamide

References:

PMID:37787768

Genotypes:

pho843delta

FYPO:0009038 - resistance to egtazic acid

References:

PMID:37787768

Genotypes:

pho843delta

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

PMID:37787768

Genotypes:

pho843delta

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

PMID:37787768

Genotypes:

pho843delta

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

PMID:37787768

Genotypes:

pho843delta

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

pho843delta

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

PMID:23697806

Genotypes:

pho843delta

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

Protein features

ID	Name	InterPro name	DB name
PF00083	Sugar_tr	MFS_sugar_transport-like	PFAM
cd17364	MFS_PhT		CDD
PS00217	SUGAR_TRANSPORT_2	Sugar_transporter_CS	PROSITE_PATTERNS
PS00216	SUGAR_TRANSPORT_1	Sugar_transporter_CS	PROSITE_PATTERNS
PS50850	MFS	MFS_dom	PROSITE_PROFILES
SSF103473	MFS general substrate transporter	MFS_trans_sf	SUPERFAMILY
G3DSA:1.20.1250.20	MFS general substrate transporter like domains	MFS_trans_sf	GENE3D
PTHR24064	SOLUTE CARRIER FAMILY 22 MEMBER		PANTHER
mobidb-lite-Disorder	disorder_prediction		MOBIDB-Disorder
mobidb-lite-Low-complexity	disorder_prediction		MOBIDB-Low-complexity
mobidb-lite-Polar	disorder_prediction		MOBIDB-Polar

Orthologs

pho843 (SJAG_04241) Schizosaccharomyces japonicus
PHO84 (YML123C) Saccharomyces cerevisiae

References / Literature

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.: Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.: Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.: Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.: Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.: Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.: Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.: Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.: Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.: Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:18257517 - Phosphoproteome analysis of fission yeast.: Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.: Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.: Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.: Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.: Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:34147496 - Regulation of inorganic polyphosphate is required for proper vacuolar proteolysis in fission yeast.: Sawada N et al. J Biol Chem 2021 Jul;297(1):100891
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.: Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.: Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514