PomBase home

protein coding gene - ura4 (SPCC330.05c) - orotidine 5'-phosphate decarboxylase Ura4

Gene summary

Standard name
ura4
Systematic ID
SPCC330.05c
Product
orotidine 5'-phosphate decarboxylase Ura4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P14965
ORFeome ID
10/10A08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 115589..116625 reverse strand

Annotation

PBO:0005012 - 4.1.1.23

Comment

PBO:0003079 - target of SAPK pathway

References:

Disease association

MONDO:0009797 - orotic aciduria

References:

GO biological process

GO:0006207 - 'de novo' pyrimidine nucleobase biosynthetic process

References:

GO:0044205 - 'de novo' UMP biosynthetic process

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO:0140453 - protein aggregate center

References:

GO molecular function

GO:0004590 - orotidine-5'-phosphate decarboxylase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0000016 - curved vegetative cell

References:

Genotypes:

FYPO:0000711 - decreased cell cycle arrest in mitotic G1 phase in response to nitrogen starvation

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0005171 - decreased uracil import

References:

Genotypes:

FYPO:0004582 - increased cell cycle arrest in mitotic G1 phase in response to nitrogen starvation

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001178 - loss of viability upon nitrogen starvation

References:

Genotypes:

FYPO:0006017 - normal growth on urea

References:

Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0001987 - sensitive to high pH

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0002865 - sensitive to polypeptone

References:

Genotypes:

FYPO:0000271 - sensitive to salt stress

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000112 - sensitive to sorbitol

References:

Genotypes:

FYPO:0006016 - sensitive to urea

References:

Genotypes:

FYPO:0002067 - slow cell population growth during recovery from stationary phase

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0000647 - vegetative cell lysis

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000027 - ribosomal density decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0001012 - growth auxotrophic for uracil

References:

Genotypes:

FYPO:0002867 - increased cellular OMP level

References:

Genotypes:

FYPO:0002868 - increased cellular orotidine level

References:

Genotypes:

FYPO:0005172 - increased uracil import

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0001178 - loss of viability upon nitrogen starvation

References:

Genotypes:

FYPO:0004675 - normal growth on dimethyl sulfoxide

References:

Genotypes:

FYPO:0002864 - resistance to 5-fluoroorotic acid

References:

Genotypes:

FYPO:0006118 - sensitive to 5-fluoroorotic acid

References:

Genotypes:

FYPO:0002988 - sensitive to ammonium

References:

Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

References:

Genotypes:

FYPO:0001190 - sensitive to cell wall-degrading enzymes

References:

Genotypes:

FYPO:0004674 - sensitive to dimethyl sulfoxide

References:

Genotypes:

FYPO:0001987 - sensitive to high pH

References:

Genotypes:

FYPO:0002865 - sensitive to polypeptone

References:

Genotypes:

FYPO:0000111 - sensitive to rapamycin

References:

Genotypes:

FYPO:0002866 - sensitive to tryptone

References:

Genotypes:

FYPO:0006016 - sensitive to urea

References:

Genotypes:

FYPO:0000647 - vegetative cell lysis

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00215OMPdecaseOMPdeCOase_domPFAM
cd04725OMP_decarboxylase_likeCDD
PS00156OMPDECASEOMPdecase_ASPROSITE_PATTERNS
SM00934OMPdecase_2OMPdeCOase_domSMART
G3DSA:3.20.20.70:FF:000114FUNFAM
SSF51366Ribulose-phoshate binding barrelRibuloseP-bd_barrelSUPERFAMILY
G3DSA:3.20.20.70Aldolase class IAldolase_TIMGENE3D
PTHR19278OROTATE PHOSPHORIBOSYLTRANSFERASEPANTHER
TIGR01740pyrFOMPdecaseNCBIFAM

Orthologs

References / Literature

PMID:26536126 - Cell Lysis in S. pombe ura4 Mutants Is Suppressed by Loss of Functional Pub1, Which Regulates the Uracil Transporter Fur4.
Nishino K et al. PLoS One 2015;10(11):e0141796
PMID:2834100 - Cloning and expression of the OMP decarboxylase gene URA4 from Schizosaccharomyces pombe.
Bach ML Curr Genet 1987;12(7):527-34
PMID:12172965 - Identifying regulators of pheromone signalling in the fission yeast Schizosaccharomyces pombe.
Didmon M et al. Curr Genet 2002 Jul;41(4):241-53
PMID:23145069 - Identification and functional analysis of the erh1(+) gene encoding enhancer of rudimentary homolog from the fission yeast Schizosaccharomyces pombe.
Krzyzanowski MK et al. PLoS One 2012;7(11):e49059
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:10233158 - Ssp1 promotes actin depolymerization and is involved in stress response and new end take-off control in fission yeast.
Rupes I et al. Mol Biol Cell 1999 May;10(5):1495-510
PMID:16762840 - Swi6/HP1 recruits a JmjC domain protein to facilitate transcription of heterochromatic repeats.
Zofall M et al. Mol Cell 2006 Jun 09;22(5):681-92
PMID:37400983 - Cooperative DNA-binding activities of Chp2 are critical for its function in heterochromatin assembly.
Rahayu AF et al. J Biochem 2023 Sep 29;174(4):371-382
PMID:6765174 - Expression of the cloned uracil permease gene of Saccharomyces cerevisiae in a heterologous membrane.
Chevallier MR et al. EMBO J 1982;1(3):375-7
PMID:9339351 - Identification of the ure1+ gene encoding urease in fission yeast.
Tange Y et al. Curr Genet 1997 Sep;32(3):244-6
PMID:17295836 - Fission yeast autophagy induced by nitrogen starvation generates a nitrogen source that drives adaptation processes.
Kohda TA et al. Genes Cells 2007 Feb;12(2):155-70
PMID:15546162 - pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast.
Matsuyama A et al. Yeast 2004 Nov;21(15):1289-305
PMID:11821054 - A DMSO-sensitive conditional mutant of the fission yeast orthologue of the Saccharomyces cerevisiae SEC13 gene is defective in septation.
Poloni D et al. FEBS Lett 2002 Jan 30;511(1-3):85-9
PMID:22992726 - The SAGA histone acetyltransferase complex regulates leucine uptake through the Agp3 permease in fission yeast.
Takahashi H et al. J Biol Chem 2012 Nov 02;287(45):38158-67
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:9211790 - Caffeine-resistance in S. pombe: mutations in three novel caf genes increase caffeine tolerance and affect radiation sensitivity, fertility, and cell cycle.
Benko Z et al. Curr Genet 1997 Jun;31(6):481-7
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:28345447 - Urea enhances cell lysis of Schizosaccharomyces pombe ura4 mutants.
Nishino K et al. Biosci Biotechnol Biochem 2017 Jul;81(7):1444-1451
PMID:29784772 - A Heterochromatin Domain Forms Gradually at a New Telomere and Is Dynamic at Stable Telomeres.
Wang J et al. Mol Cell Biol 2018 Aug 01;38(15)
PMID:22426534 - DNA replication through hard-to-replicate sites, including both highly transcribed RNA Pol II and Pol III genes, requires the S. pombe Pfh1 helicase.
Sabouri N et al. Genes Dev 2012 Mar 15;26(6):581-93
PMID:10487925 - Gene disruption in Schizosaccharomyces pombe using a temperature-sensitive Ura4p.
Davis K et al. Yeast 1999 Sep 15;15(12):1231-6
PMID:12521309 - Post-transcriptional regulation of ura4+ gene expression by glucose in Schizosaccharomyces pombe.
Kim MJ et al. Mol Cells 2002 Dec 31;14(3):437-43
PMID:16115814 - Pas1, a G1 cyclin, regulates amino acid uptake and rescues a delay in G1 arrest in Tsc1 and Tsc2 mutants in Schizosaccharomyces pombe.
van Slegtenhorst M et al. Hum Mol Genet 2005 Oct 01;14(19):2851-8
PMID:32075773 - Chaperone-Facilitated Aggregation of Thermo-Sensitive Proteins Shields Them from Degradation during Heat Stress.
Cabrera M et al. Cell Rep 2020 Feb 18;30(7):2430-2443.e4
PMID:38415071 - The Cdc14 phosphatase, Clp1, does not affect genome expression.
Lopez Maury L et al. MicroPubl Biol 2024;2024
PMID:39945308 - Characterization of the Swi6/HP1 binding motif in its partner protein reveals the basis for the functional divergence of the HP1 family proteins in fission yeast.
Oya T et al. FASEB J 2025 Feb 28;39(4):e70387
PMID:3241625 - Observations on integrative transformation in Schizosaccharomyces pombe.
Grimm C et al. Mol Gen Genet 1988 Dec;215(1):87-93
PMID:23771057 - Sir2 is required for Clr4 to initiate centromeric heterochromatin assembly in fission yeast.
Alper BJ et al. EMBO J 2013 Aug 28;32(17):2321-35
PMID:39527204 - Targeted Forward Genetics: Saturating Mutational Analyses of Specific Target Loci Within the Genome.
Protacio RU et al. Methods Mol Biol 2025;2862:223-239
PMID:23555823 - Polypeptone induces dramatic cell lysis in ura4 deletion mutants of fission yeast.
Matsuo Y et al. PLoS One 2013;8(3):e59887
PMID:2806887 - Meiotic recombination-deficient mutants of Schizosaccharomyces pombe.
Ponticelli AS et al. Genetics 1989 Sep;123(1):45-54
PMID:27613427 - Stress sensitivity of a fission yeast strain lacking histidine kinases is rescued by the ectopic expression of Chk1 from Candida albicans.
Maksimov V et al. Curr Genet 2017 May;63(2):343-357
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:39132053 - Distance-dependent effects on CRISPR/Cas9-mediated genome editing in Schizosaccharomyces pombe compromise efficiency and create unsought alleles.
Protacio RU et al. MicroPubl Biol 2024;2024
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:17248775 - Genetic Mapping in SCHIZOSACCHAROMYCES POMBE by Mitotic and Meiotic Analysis and Induced Haploidization.
Kohli J et al. Genetics 1977 Nov;87(3):471-89
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:8493104 - Study of multiple fibrillarin mRNAs reveals that 3' end formation in Schizosaccharomyces pombe is sensitive to cold shock.
Girard JP et al. Nucleic Acids Res 1993 Apr 25;21(8):1881-7
PMID:38440330 - DNA sequences and distinct mechanisms for ura4-595 and ura4-294 alleles of S. pombe .
Protacio RU et al. MicroPubl Biol 2024;2024
PMID:9822592 - Localization of the 26S proteasome during mitosis and meiosis in fission yeast.
Wilkinson CR et al. EMBO J 1998 Nov 16;17(22):6465-76
PMID:15466417 - Regulation of leucine uptake by tor1+ in Schizosaccharomyces pombe is sensitive to rapamycin.
Weisman R et al. Genetics 2005 Feb;169(2):539-50
PMID:8625425 - pH sensitivity of Schizosaccharomyces pombe: effect on the cellular phenotype associated with lacZ gene expression.
Arndt GM et al. Curr Genet 1996 Apr;29(5):457-61
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:14718525 - Tsc1+ and tsc2+ regulate arginine uptake and metabolism in Schizosaccharomyces pombe.
van Slegtenhorst M et al. J Biol Chem 2004 Mar 26;279(13):12706-13