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protein coding gene - mug27 (SPCC417.06c) - meiosis specific Sid2-related serine/threonine protein kinase (NDR family) Mug27/Slk1

Gene summary

Standard name
mug27
Systematic ID
SPCC417.06c
Product
meiosis specific Sid2-related serine/threonine protein kinase (NDR family) Mug27/Slk1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ppk35, slk1
UniProt ID
O94487
ORFeome ID
27/27B04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1677093..1679436 reverse strand

Annotation

GO biological process

GO:0032120 - ascospore-type prospore membrane formation

References:

GO:0035556 - intracellular signal transduction

References:

GO:0140043 - lipid droplet localization to prospore membrane leading edge

References:

GO:1903023 - regulation of ascospore-type prospore membrane formation

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0035974 - meiotic spindle pole body

References:

GO:0005730 - nucleolus

References:

GO:0005634 - nucleus

References:

GO:0005628 - prospore membrane

References:

GO molecular function

GO:0004674 - protein serine/threonine kinase activity

References:

Genome organisation

PBO:0000359 - antisense transcript detected

Modification

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0003066 - abnormal sporulation resulting in formation of ascus with fewer than four spores

References:

Genotypes:

FYPO:0002708 - abolished prospore formation

References:

Genotypes:

FYPO:0000583 - abolished sporulation

References:

Genotypes:

FYPO:0007170 - normal number of nuclei in ascus

References:

Genotypes:

FYPO:0003845 - normal protein localization to prospore membrane

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Genotypes:

FYPO:0000943 - normal spore morphology

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Genotypes:

FYPO:0000590 - normal sporulation

References:

Genotypes:

FYPO:0006777 - prospore membrane formation excluding nucleus

References:

Genotypes:

Protein features

PBO:0111753 - Ndr kinase/myotonic dystrophy kinase/cot1/warts family

References:

PBO:0114458 - Sid2-Like Kinase

References:

Qualitative gene expression

PomGeneEx:0000022 - protein absent

References:

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000021 - protein present

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0004953 - abnormal prospore membrane

References:

Genotypes:

FYPO:0001914 - abnormal prospore membrane formation

References:

Genotypes:

FYPO:0003561 - abnormal prospore-specific spindle pole body remodeling

References:

Genotypes:

FYPO:0003139 - abnormal sporulation resulting in formation of ascus containing anucleate spores

References:

Genotypes:

FYPO:0003066 - abnormal sporulation resulting in formation of ascus with fewer than four spores

References:

Genotypes:

FYPO:0006058 - abnormal vacuole fusion during sporulation

References:

Genotypes:

FYPO:0004484 - abolished protein localization to prospore membrane

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

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Genotypes:

FYPO:0005737 - delayed onset of prospore membrane formation

References:

Genotypes:

FYPO:0004952 - incomplete prospore membrane closure

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

References:

Genotypes:

FYPO:0006053 - lipid droplets present in spore cytoplasm in decreased numbers, mislocalized to ascus epiplasm

References:

Genotypes:

FYPO:0006003 - normal cellular component organization

References:

Genotypes:

FYPO:0000478 - normal meiosis

References:

Genotypes:

FYPO:0003790 - normal protein localization during meiosis

References:

Genotypes:

FYPO:0003541 - normal protein localization to meiotic spindle pole body

References:

Genotypes:

FYPO:0006055 - normal protein localization to meiotic spindle pole body during meiosis II

References:

Genotypes:

FYPO:0003845 - normal protein localization to prospore membrane

References:

Genotypes:

FYPO:0006777 - prospore membrane formation excluding nucleus

References:

Genotypes:

FYPO:0000073 - resistance to caffeine

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0002578 - resistance to hydroxyurea

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0002167 - sensitive to ethyl methanesulfonate

References:

Genotypes:

FYPO:0000107 - sensitive to latrunculin A

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0000346 - small spores

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00069PkinaseProt_kinase_domPFAM
PF00433Pkinase_CPkinase_CPFAM
cd05600STKc_Sid2p_likeCDD
cd21776MobB_Sid2p-likeCDD
PS00107PROTEIN_KINASE_ATPProtein_kinase_ATP_BSPROSITE_PATTERNS
PS00108PROTEIN_KINASE_STSer/Thr_kinase_ASPROSITE_PATTERNS
PS50011PROTEIN_KINASE_DOMProt_kinase_domPROSITE_PROFILES
PS51285AGC_KINASE_CTERAGC-kinase_CPROSITE_PROFILES
SM00220serkin_6Prot_kinase_domSMART
SM00133pkinase_C_6AGC-kinase_CSMART
G3DSA:1.10.510.10:FF:000319FUNFAM
G3DSA:3.30.200.20:FF:000109FUNFAM
G3DSA:1.10.510.10:FF:000141FUNFAM
SSF56112Protein kinase-like (PK-like)Kinase-like_dom_sfSUPERFAMILY
G3DSA:1.10.510.10Transferase(Phosphotransferase) domain 1GENE3D
G3DSA:3.30.200.20Phosphorylase Kinase; domain 1GENE3D
PTHR24356SERINE/THREONINE-PROTEIN KINASESer_Thr_kinase_AGCPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMOBIDB-Positive-Polyelectrolyte

Orthologs

References / Literature

PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:18411246 - Mug27 is a meiosis-specific protein kinase that functions in fission yeast meiosis II and sporulation.
Ohtaka A et al. J Cell Sci 2008 May 01;121(Pt 9):1547-58
PMID:18562696 - The meiosis-specific Sid2p-related protein Slk1p regulates forespore membrane assembly in fission yeast.
Yan H et al. Mol Biol Cell 2008 Sep;19(9):3676-90
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:20826461 - The role of Schizosaccharomyces pombe dma1 in spore formation during meiosis.
Krapp A et al. J Cell Sci 2010 Oct 01;123(Pt 19):3284-93
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:16303567 - A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events.
Martín-Castellanos C et al. Curr Biol 2005 Nov 22;15(22):2056-62
PMID:23370392 - A knockout screen for protein kinases required for the proper meiotic segregation of chromosomes in the fission yeast Schizosaccharomyces pombe.
Kovacikova I et al. Cell Cycle 2013 Feb 15;12(4):618-24
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28011631 - Lipid droplet dynamics during Schizosaccharomyces pombe sporulation and their role in spore survival.
Yang HJ et al. Biol Open 2017 Feb 15;6(2):217-222
PMID:28876998 - Meiosis-specific localization of the exocytic Rab Ypt2 in fission yeast.
Imada K et al. Small GTPases 2020 Mar;11(2):146-154
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20980623 - A novel role of Dma1 in regulating forespore membrane assembly and sporulation in fission yeast.
Li WZ et al. Mol Biol Cell 2010 Dec;21(24):4349-60
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:18397994 - Slk1 is a meiosis-specific Sid2-related kinase that coordinates meiotic nuclear division with growth of the forespore membrane.
Pérez-Hidalgo L et al. J Cell Sci 2008 May 01;121(Pt 9):1383-92
PMID:15821139 - Systematic deletion analysis of fission yeast protein kinases.
Bimbó A et al. Eukaryot Cell 2005 Apr;4(4):799-813
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160