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protein coding gene - lsg1 (SPCC4B3.08) - Lsk1 complex gamma subunit Lsg1

Gene summary

Standard name
lsg1
Systematic ID
SPCC4B3.08
Product
Lsk1 complex gamma subunit Lsg1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ctk3
UniProt ID
Q9USJ8
ORFeome ID
07/07F01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1161241..1162288 reverse strand

Annotation

Comment

PBO:0024869 - does not bind rpb1 CTD peptides in vitro

References:

GO biological process

GO:0045943 - positive regulation of transcription by RNA polymerase I

References:

GO:0045944 - positive regulation of transcription by RNA polymerase II

References:

GO:0032968 - positive regulation of transcription elongation by RNA polymerase II

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GO cellular component

GO:0070692 - CTDK-1 complex

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

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GO molecular function

GO:0005509 - calcium ion binding

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Multi-locus phenotype

FYPO:0001147 - normal mating efficiency

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000020 - protein level unchanged

References:

PomGeneEx:0000021 - protein present

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000031 - abnormal mating

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Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0000625 - abnormal premeiotic DNA replication

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Genotypes:

FYPO:0005038 - abolished phosphorylation of RNA polymerase II C-terminal domain serine 2 residues during nitrogen starvation

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Genotypes:

FYPO:0001585 - abolished protein localization to cell tip during vegetative growth

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Genotypes:

FYPO:0001800 - abolished protein localization to cell tip, with protein distributed in plasma membrane or cortex

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Genotypes:

FYPO:0009078 - decreased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

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Genotypes:

FYPO:0000303 - decreased conjugation frequency

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Genotypes:

FYPO:0004461 - decreased level of regulation of sexual differentiation gene mRNA during nitrogen starvation

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0000470 - decreased mating type switching

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Genotypes:

FYPO:0004067 - decreased phosphorylation of RNA polymerase II C-terminal domain serine 2 residues during vegetative growth

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Genotypes:

FYPO:0000538 - decreased protein secretion during vegetative growth

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Genotypes:

FYPO:0000584 - decreased sporulation frequency

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0000712 - delayed onset of cell cycle arrest in mitotic G1 phase in response to nitrogen starvation

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Genotypes:

FYPO:0004045 - elongated tetranucleate vegetative cell with fragmented septum

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Genotypes:

FYPO:0004158 - elongated vegetative cell with fragmented septum and more than four nuclei

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0001908 - increased pre-mRNA level

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0005037 - normal phosphorylation of RNA polymerase II C-terminal domain serine 5 residues during nitrogen starvation

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Genotypes:

FYPO:0009030 - resistance to amitrole

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002767 - resistance to terbinafine

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0000085 - sensitive to camptothecin

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000107 - sensitive to latrunculin A

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002641 - sensitive to micafungin

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000750 - sensitive to silver ions

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Genotypes:

FYPO:0006929 - sensitive to silver nanoparticles

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Genotypes:

FYPO:0005889 - sensitive to sodium chloride

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Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000022 - shmoo with long tip

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

FYPO:0000281 - small vacuoles present in increased numbers during vegetative growth

References:

Genotypes:

FYPO:0000024 - stubby vegetative cell

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF12350CTK3_CCtk3_CPFAM
PF12243CTK3Ctk3_NPFAM
PS51391CIDCID_domPROSITE_PROFILES
G3DSA:1.25.40.90:FF:000032FUNFAM
G3DSA:1.25.40.90ENTH_VHSGENE3D
PTHR28291CTD KINASE SUBUNIT GAMMACtk3PANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

Orthologs

References / Literature

PMID:21931816 - Global gene expression analysis of fission yeast mutants impaired in Ser-2 phosphorylation of the RNA pol II carboxy terminal domain.
Saberianfar R et al. PLoS One 2011;6(9):e24694
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28945192 - Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe .
Guydosh NR et al. Elife 2017 Sep 25;6
PMID:26219431 - Structure of Ctk3, a subunit of the RNA polymerase II CTD kinase complex, reveals a noncanonical CTD-interacting domain fold.
Mühlbacher W et al. Proteins 2015 Oct;83(10):1849-58
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:27172183 - Interconnections Between RNA-Processing Pathways Revealed by a Sequencing-Based Genetic Screen for Pre-mRNA Splicing Mutants in Fission Yeast.
Larson A et al. G3 (Bethesda) 2016 Jun 01;6(6):1513-23
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:20605454 - A gene-specific requirement of RNA polymerase II CTD phosphorylation for sexual differentiation in S. pombe.
Coudreuse D et al. Curr Biol 2010 Jun 22;20(12):1053-64
PMID:21850271 - Genome-wide screening for genes associated with FK506 sensitivity in fission yeast.
Ma Y et al. PLoS One 2011;6(8):e23422
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:22144909 - The fission yeast stress-responsive MAPK pathway promotes meiosis via the phosphorylation of Pol II CTD in response to environmental and feedback cues.
Sukegawa Y et al. PLoS Genet 2011 Dec;7(12):e1002387
GO_REF:0000051 - S. pombe keyword mapping
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:29294138 - Editor's Highlight: A Genome-wide Screening of Target Genes Against Silver Nanoparticles in Fission Yeast.
Lee AR et al. Toxicol Sci 2018 Jan 01;161(1):171-185