PomBase home

protein coding gene - lsg1 (SPCC4B3.08) - Lsk1 complex gamma subunit Lsg1

Gene summary

Standard name
lsg1
Systematic ID
SPCC4B3.08
Product
Lsk1 complex gamma subunit Lsg1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ctk3
UniProt ID
Q9USJ8
ORFeome ID
07/07F01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1161241..1162288 reverse strand

Annotation

Comment

PBO:0024869 - does not bind rpb1 CTD peptides in vitro

References:

GO biological process

GO:0045943 - positive regulation of transcription by RNA polymerase I

References:

GO:0045944 - positive regulation of transcription by RNA polymerase II

References:

GO:0032968 - positive regulation of transcription elongation by RNA polymerase II

References:

GO cellular component

GO:0070692 - CTDK-1 complex

References:

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005509 - calcium ion binding

References:

Multi-locus phenotype

FYPO:0001147 - normal mating efficiency

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000020 - protein level unchanged

References:

PomGeneEx:0000021 - protein present

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000031 - abnormal mating

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000625 - abnormal premeiotic DNA replication

References:

Genotypes:

FYPO:0005038 - abolished phosphorylation of RNA polymerase II C-terminal domain serine 2 residues during nitrogen starvation

References:

Genotypes:

FYPO:0001585 - abolished protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0001800 - abolished protein localization to cell tip, with protein distributed in plasma membrane or cortex

References:

Genotypes:

FYPO:0009078 - decreased cell population growth on ethanol carbon source

References:

Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0000303 - decreased conjugation frequency

References:

Genotypes:

FYPO:0004461 - decreased level of regulation of sexual differentiation gene mRNA during nitrogen starvation

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0004067 - decreased phosphorylation of RNA polymerase II C-terminal domain serine 2 residues during vegetative growth

References:

Genotypes:

FYPO:0000538 - decreased protein secretion during vegetative growth

References:

Genotypes:

FYPO:0000584 - decreased sporulation frequency

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000712 - delayed onset of cell cycle arrest in mitotic G1 phase in response to nitrogen starvation

References:

Genotypes:

FYPO:0004045 - elongated tetranucleate vegetative cell with fragmented septum

References:

Genotypes:

FYPO:0004158 - elongated vegetative cell with fragmented septum and more than four nuclei

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0001908 - increased pre-mRNA level

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0005037 - normal phosphorylation of RNA polymerase II C-terminal domain serine 5 residues during nitrogen starvation

References:

Genotypes:

FYPO:0009030 - resistance to amitrole

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0002578 - resistance to hydroxyurea

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002767 - resistance to terbinafine

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000107 - sensitive to latrunculin A

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002641 - sensitive to micafungin

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000750 - sensitive to silver ions

References:

Genotypes:

FYPO:0006929 - sensitive to silver nanoparticles

References:

Genotypes:

FYPO:0005889 - sensitive to sodium chloride

References:

Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000022 - shmoo with long tip

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0000281 - small vacuoles present in increased numbers during vegetative growth

References:

Genotypes:

FYPO:0000024 - stubby vegetative cell

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF12243CTK3Ctk3_NPfam
PF12350CTK3_CCtk3_CPfam
PS51391CIDCID_domPROSITE profiles
G3DSA:1.25.40.90:FF:000032CATH-FunFam
G3DSA:1.25.40.90ENTH_VHSCATH-Gene3D
PTHR28291Ctk3PANTHER
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21850271 - Genome-wide screening for genes associated with FK506 sensitivity in fission yeast.
Ma Y et al. PLoS One 2011;6(8):e23422
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:28945192 - Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe .
Guydosh NR et al. Elife 2017 Sep 25;6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:20605454 - A gene-specific requirement of RNA polymerase II CTD phosphorylation for sexual differentiation in S. pombe.
Coudreuse D et al. Curr Biol 2010 Jun 22;20(12):1053-64
PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21931816 - Global gene expression analysis of fission yeast mutants impaired in Ser-2 phosphorylation of the RNA pol II carboxy terminal domain.
Saberianfar R et al. PLoS One 2011;6(9):e24694
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:26219431 - Structure of Ctk3, a subunit of the RNA polymerase II CTD kinase complex, reveals a noncanonical CTD-interacting domain fold.
Mühlbacher W et al. Proteins 2015 Oct;83(10):1849-58
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:27172183 - Interconnections Between RNA-Processing Pathways Revealed by a Sequencing-Based Genetic Screen for Pre-mRNA Splicing Mutants in Fission Yeast.
Larson A et al. G3 (Bethesda) 2016 Jun 01;6(6):1513-23
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
GO_REF:0000051 - S. pombe keyword mapping
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:22144909 - The fission yeast stress-responsive MAPK pathway promotes meiosis via the phosphorylation of Pol II CTD in response to environmental and feedback cues.
Sukegawa Y et al. PLoS Genet 2011 Dec;7(12):e1002387
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:29294138 - Editor's Highlight: A Genome-wide Screening of Target Genes Against Silver Nanoparticles in Fission Yeast.
Lee AR et al. Toxicol Sci 2018 Jan 01;161(1):171-185
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603