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protein coding gene - aph1 (SPCC4G3.02) - bis(5'-nucleosidyl)-tetraphosphatase

Gene summary

Standard name
aph1
Systematic ID
SPCC4G3.02
Product
bis(5'-nucleosidyl)-tetraphosphatase
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P49776
ORFeome ID
11/11B07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 468323..469727 reverse strand

Annotation

PBO:0005148 - 3.6.1.17

GO biological process

GO:0015964 - diadenosine triphosphate catabolic process

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GO cellular component

GO:0005829 - cytosol

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GO:0005739 - mitochondrion

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GO:0005634 - nucleus

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GO molecular function

GO:0004081 - bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity

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Modification

MOD:00046 - O-phospho-L-serine

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Multi-locus phenotype

FYPO:0000229 - cut

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Genotypes:

FYPO:0005094 - decreased duration of S-phase DNA damage checkpoint during cellular response to hydroxyurea

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Genotypes:

FYPO:0002096 - increased protein phosphorylation during cellular response to hydroxyurea

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

Protein features

PBO:0111932 - HIT family

Qualitative gene expression

PomGeneEx:0000022 - protein absent

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PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000021 - protein present

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000006 - abnormal mitotic DNA damage checkpoint

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Genotypes:

FYPO:0006582 - decreased bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity

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Genotypes:

FYPO:0005094 - decreased duration of S-phase DNA damage checkpoint during cellular response to hydroxyurea

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

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Genotypes:

FYPO:0004645 - increased bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity

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Genotypes:

FYPO:0006577 - increased cellular P(1),P(4)-bis(5'-adenosyl) tetraphosphate level

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01230HITHIT-likePfam
cd01275FHITFHITCDD
PS00892HIT_1Histidine_triad_CSPROSITE patterns
PS51084HIT_2HIT-likePROSITE profiles
PR00332HISTRIADHistidine_triad_HITPRINTS
G3DSA:3.30.428.10:FF:000011CATH-FunFam
G3DSA:3.30.428.10HIT-like_sfCATH-Gene3D
SSF54197HIT-like_sfSUPERFAMILY
PTHR46243Bis(5'-adenosyl)-TPase_regPANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:28945192 - Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe .
Guydosh NR et al. Elife 2017 Sep 25;6
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:8554540 - Cloning of the Schizosaccharomyces pombe gene encoding diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A) asymmetrical hydrolase: sequence similarity with the histidine triad (HIT) protein family.
Huang Y et al. Biochem J 1995 Dec 15;312 ( Pt 3)(Pt 3):925-32
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:10970777 - Disruption and overexpression of the Schizosaccharomyces pombe aph1 gene and the effects on intracellular diadenosine 5',5'''-P1, P4-tetraphosphate (Ap4A), ATP and ADP concentrations.
Ingram SW et al. Biochem J 2000 Sep 15;350 Pt 3(Pt 3):663-9
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:31538680 - The fission yeast FHIT homolog affects checkpoint control of proliferation and is regulated by mitochondrial electron transport.
Sjölander JJ et al. Cell Biol Int 2020 Feb;44(2):412-423
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36