protein coding gene - mus81 (SPCC4G3.05c) - Holliday junction resolvase subunit Mus81

Gene summary

Standard name

mus81

Systematic ID

SPCC4G3.05c

Product

Holliday junction resolvase subunit Mus81

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

slx3

UniProt ID

P87231

ORFeome ID

35/35F02

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome III: 462720..464714 forward strand

Annotation

Protein features

Orthologs

• MUS81 (HGNC:29814) Homo sapiens
• mus81 (SJAG_01044) Schizosaccharomyces japonicus
• MUS81 (YDR386W) Saccharomyces cerevisiae

References / Literature

PMID:24312672 - A quality control mechanism linking meiotic success to release of ascospores.

Guo H et al. PLoS One 2013;8(12):e82758

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:11073977 - Damage tolerance protein Mus81 associates with the FHA1 domain of checkpoint kinase Cds1.

Boddy MN et al. Mol Cell Biol 2000 Dec;20(23):8758-66

PMID:28586299 - Inter-Fork Strand Annealing causes genomic deletions during the termination of DNA replication.

Morrow CA et al. Elife 2017 Jun 06;6

GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference

PMID:15466419 - Swi5 acts in meiotic DNA joint molecule formation in Schizosaccharomyces pombe.

Ellermeier C et al. Genetics 2004 Dec;168(4):1891-8

PMID:28922417 - Lingering single-strand breaks trigger Rad51-independent homology-directed repair of collapsed replication forks in the polynucleotide kinase/phosphatase mutant of fission yeast.

Sanchez A et al. PLoS Genet 2017 Sep;13(9):e1007013

PMID:29084823 - Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast.

Navarro FJ et al. J Cell Sci 2017 Dec 15;130(24):4144-4154

PMID:11719193 - Mus81-Eme1 are essential components of a Holliday junction resolvase.

Boddy MN et al. Cell 2001 Nov 16;107(4):537-48

PMID:30148840 - Tdp1 processes chromate-induced single-strand DNA breaks that collapse replication forks.

Ganguly A et al. PLoS Genet 2018 Aug;14(8):e1007595

PMID:18388861 - Mus81 is essential for sister chromatid recombination at broken replication forks.

Roseaulin L et al. EMBO J 2008 May 07;27(9):1378-87

PMID:21444718 - DNA repair and global sumoylation are regulated by distinct Ubc9 noncovalent complexes.

Prudden J et al. Mol Cell Biol 2011 Jun;31(11):2299-310

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:27473316 - Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.

Ranatunga NS et al. G3 (Bethesda) 2016 Oct 13;6(10):3049-3063

PMID:38718864 - H2B oncohistones cause homologous recombination defect and genomic instability through reducing H2B monoubiquitination in Schizosaccharomyces pombe.

Qin B et al. J Biol Chem 2024 May 06;:107345

PMID:14527419 - The endogenous Mus81-Eme1 complex resolves Holliday junctions by a nick and counternick mechanism.

Gaillard PHL et al. Mol Cell 2003 Sep;12(3):747-59

PMID:20655467 - Crossover invariance determined by partner choice for meiotic DNA break repair.

Hyppa RW et al. Cell 2010 Jul 23;142(2):243-55

PMID:32341083 - Active Replication Checkpoint Drives Genome Instability in Fission Yeast mcm4 Mutant.

Kim SM et al. Mol Cell Biol 2020 Jun 29;40(14)

PMID:24748152 - Fission yeast Rad52 phosphorylation restrains error prone recombination pathways.

Bellini A et al. PLoS One 2014;9(4):e95788

GO_REF:0000002 - Comments

PMID:23703609 - TORC2 is required to maintain genome stability during S phase in fission yeast.

Schonbrun M et al. J Biol Chem 2013 Jul 05;288(27):19649-60

PMID:15367656 - Swi1 and Swi3 are components of a replication fork protection complex in fission yeast.

Noguchi E et al. Mol Cell Biol 2004 Oct;24(19):8342-55

PMID:21084840 - The Mek1 phosphorylation cascade plays a role in meiotic recombination of Schizosaccharomyces pombe.

Tougan T et al. Cell Cycle 2010 Dec 01;9(23):4688-702

PMID:17307401 - The novel gene mus7(+) is involved in the repair of replication-associated DNA damage in fission yeast.

Yokoyama M et al. DNA Repair (Amst) 2007 Jun 01;6(6):770-80

PMID:26368543 - Critical Function of γH2A in S-Phase.

Mejia-Ramirez E et al. PLoS Genet 2015 Sep;11(9):e1005517

PMID:25414342 - Rad51/Dmc1 paralogs and mediators oppose DNA helicases to limit hybrid DNA formation and promote crossovers during meiotic recombination.

Lorenz A et al. Nucleic Acids Res 2014 Dec 16;42(22):13723-35

PMID:16478984 - The Nse5-Nse6 dimer mediates DNA repair roles of the Smc5-Smc6 complex.

Pebernard S et al. Mol Cell Biol 2006 Mar;26(5):1617-30

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:22927644 - Replication fork collapse and genome instability in a deoxycytidylate deaminase mutant.

Sánchez A et al. Mol Cell Biol 2012 Nov;32(21):4445-54

PMID:30667359 - Factors affecting template switch recombination associated with restarted DNA replication.

Jalan M et al. Elife 2019 Jan 22;8

PMID:18667534 - Activation of the DNA damage checkpoint in mutants defective in DNA replication initiation.

Yin L et al. Mol Biol Cell 2008 Oct;19(10):4374-82

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:19037101 - Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint.

Willis N et al. Mol Biol Cell 2009 Feb;20(3):819-33

PMID:29898918 - Mutations that prevent methylation of cohesin render sensitivity to DNA damage in S. pombe .

Sanyal S et al. J Cell Sci 2018 Jul 06;131(13)

PMID:41525994 - Transcriptional PBR cycles at pericentromeric repeats cause gross chromosomal rearrangements through Rad52-dependent ADR-loop formation.

Xu R et al. Nucleic Acids Res 2026 Jan 05;54(1)

PMID:22723423 - The fission yeast FANCM ortholog directs non-crossover recombination during meiosis.

Lorenz A et al. Science 2012 Jun 22;336(6088):1585-8

PMID:14993467 - The involvement of Srs2 in post-replication repair and homologous recombination in fission yeast.

Doe CL et al. Nucleic Acids Res 2004;32(4):1480-91

PMID:17724078 - Break-induced loss of heterozygosity in fission yeast: dual roles for homologous recombination in promoting translocations and preventing de novo telomere addition.

Cullen JK et al. Mol Cell Biol 2007 Nov;27(21):7745-57

PMID:22645654 - Opposing role of condensin hinge against replication protein A in mitosis and interphase through promoting DNA annealing.

Akai Y et al. Open Biol 2011 Dec;1(4):110023

PMID:25293972 - Increased meiotic crossovers and reduced genome stability in absence of Schizosaccharomyces pombe Rad16 (XPF).

Mastro TL et al. Genetics 2014 Dec;198(4):1457-72

PMID:34292936 - Fission yeast Rad8/HLTF facilitates Rad52-dependent chromosomal rearrangements through PCNA lysine 107 ubiquitination.

Su J et al. PLoS Genet 2021 Jul;17(7):e1009671

PMID:32355220 - DNA replication machinery prevents Rad52-dependent single-strand annealing that leads to gross chromosomal rearrangements at centromeres.

Onaka AT et al. Commun Biol 2020 Apr 30;3(1):202

PMID:40594858 - Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions.

Bakosova A et al. Sci Rep 2025 Jul 01;15(1):21895

PMID:27806301 - Meiotic Nuclear Oscillations Are Necessary to Avoid Excessive Chromosome Associations.

Chacón MR et al. Cell Rep 2016 Nov 01;17(6):1632-1645

PMID:11741546 - Human Mus81-associated endonuclease cleaves Holliday junctions in vitro.

Chen XB et al. Mol Cell 2001 Nov;8(5):1117-27

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:16710300 - Sws1 is a conserved regulator of homologous recombination in eukaryotic cells.

Martín V et al. EMBO J 2006 Jun 07;25(11):2564-74

PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.

Mata J et al. Nat Genet 2002 Sep;32(1):143-7

PMID:14704204 - Fission yeast Mus81.Eme1 Holliday junction resolvase is required for meiotic crossing over but not for gene conversion.

Smith GR et al. Genetics 2003 Dec;165(4):2289-93

PMID:19470480 - Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required for efficient meiotic recombination.

Farah JA et al. Proc Natl Acad Sci U S A 2009 Jun 09;106(23):9356-61

PMID:19363481 - Molecular mimicry of SUMO promotes DNA repair.

Prudden J et al. Nat Struct Mol Biol 2009 May;16(5):509-16

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:12084712 - Mus81-Eme1 and Rqh1 involvement in processing stalled and collapsed replication forks.

Doe CL et al. J Biol Chem 2002 Sep 06;277(36):32753-9

PMID:15486206 - DNA repair by a Rad22-Mus81-dependent pathway that is independent of Rhp51.

Doe CL et al. Nucleic Acids Res 2004;32(18):5570-81

PMID:15654094 - A novel recombination pathway initiated by the Mre11/Rad50/Nbs1 complex eliminates palindromes during meiosis in Schizosaccharomyces pombe.

Farah JA et al. Genetics 2005 Mar;169(3):1261-74

PMID:20885790 - Critical functions of Rpa3/Ssb3 in S-phase DNA damage responses in fission yeast.

Cavero S et al. PLoS Genet 2010 Sep 23;6(9):e1001138

PMID:22064477 - The RecQ4 orthologue Hrq1 is critical for DNA interstrand cross-link repair and genome stability in fission yeast.

Groocock LM et al. Mol Cell Biol 2012 Jan;32(2):276-87

PMID:22855558 - Meiotic DNA joint molecule resolution depends on Nse5-Nse6 of the Smc5-Smc6 holocomplex.

Wehrkamp-Richter S et al. Nucleic Acids Res 2012 Oct;40(19):9633-46

PMID:23982516 - A winged helix domain in human MUS81 binds DNA and modulates the endonuclease activity of MUS81 complexes.

Fadden AJ et al. Nucleic Acids Res 2013 Nov;41(21):9741-52

PMID:24861625 - Hyperactive Cdc2 kinase interferes with the response to broken replication forks by trapping S.pombe Crb2 in its mitotic T215 phosphorylated state.

Mahyous Saeyd SA et al. Nucleic Acids Res 2014 Jul;42(12):7734-47

PMID:25533348 - Tolerance of deregulated G1/S transcription depends on critical G1/S regulon genes to prevent catastrophic genome instability.

Caetano C et al. Cell Rep 2014 Dec 24;9(6):2279-89

PMID:27398807 - Functional Crosstalk between the PP2A and SUMO Pathways Revealed by Analysis of STUbL Suppressor, razor 1-1.

Nie M et al. PLoS Genet 2016 Jul;12(7):e1006165

PMID:27697832 - Rad51 and Rad54 promote noncrossover recombination between centromere repeats on the same chromatid to prevent isochromosome formation.

Onaka AT et al. Nucleic Acids Res 2016 Dec 15;44(22):10744-10757

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:29550859 - Genetic interactions between the chromosome axis-associated protein Hop1 and homologous recombination determinants in Schizosaccharomyces pombe.

Brown SD et al. Curr Genet 2018 Oct;64(5):1089-1104

PMID:33511417 - Repression of a large number of genes requires interplay between homologous recombination and HIRA.

Misova I et al. Nucleic Acids Res 2021 Feb 26;49(4):1914-1934

PMID:12589755 - The fission yeast spSet1p is a histone H3-K4 methyltransferase that functions in telomere maintenance and DNA repair in an ATM kinase Rad3-dependent pathway.

Kanoh J et al. J Mol Biol 2003 Feb 28;326(4):1081-94

PMID:15805465 - Replication checkpoint kinase Cds1 regulates Mus81 to preserve genome integrity during replication stress.

Kai M et al. Genes Dev 2005 Apr 15;19(8):919-32

PMID:20040574 - The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant.

Lorenz A et al. Nucleic Acids Res 2010 Apr;38(6):1866-73

PMID:25965521 - Ku stabilizes replication forks in the absence of Brc1.

Sánchez A et al. PLoS One 2015;10(5):e0126598

PMID:15972456 - Brc1-mediated DNA repair and damage tolerance.

Sheedy DM et al. Genetics 2005 Oct;171(2):457-68

PMID:24875629 - Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.

Ding L et al. G3 (Bethesda) 2014 May 28;4(8):1373-84

PMID:29622660 - RNase H eliminates R-loops that disrupt DNA replication but is nonessential for efficient DSB repair.

Zhao H et al. EMBO Rep 2018 May;19(5)

PMID:23478021 - Replication fork stability is essential for the maintenance of centromere integrity in the absence of heterochromatin.

Li PC et al. Cell Rep 2013 Mar 28;3(3):638-45

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:12897162 - Replication checkpoint kinase Cds1 regulates recombinational repair protein Rad60.

Boddy MN et al. Mol Cell Biol 2003 Aug;23(16):5939-46

PMID:21183410 - Schizosaccharomyces pombe Mms1 channels repair of perturbed replication into Rhp51 independent homologous recombination.

Vejrup-Hansen R et al. DNA Repair (Amst) 2011 Mar 07;10(3):283-95

PMID:22426535 - The DNA helicase Pfh1 promotes fork merging at replication termination sites to ensure genome stability.

Steinacher R et al. Genes Dev 2012 Mar 15;26(6):594-602

PMID:15238514 - Conserved and nonconserved proteins for meiotic DNA breakage and repair in yeasts.

Young JA et al. Genetics 2004 Jun;167(2):593-605

PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.

Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684

PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.

Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721

PMID:18667531 - Nse1 RING-like domain supports functions of the Smc5-Smc6 holocomplex in genome stability.

Pebernard S et al. Mol Biol Cell 2008 Oct;19(10):4099-109

PMID:24089141 - DNA intermediates of meiotic recombination in synchronous S. pombe at optimal temperature.

Hyppa RW et al. Nucleic Acids Res 2014 Jan;42(1):359-69

GO_REF:0000117 - Electronic Gene Ontology annotations created by ARBA machine learning models

PMID:22907753 - Posttranscriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in Schizosaccharomyces pombe.

Wells ML et al. Mol Cell Biol 2012 Oct;32(20):4206-14

PMID:15485909 - Rad62 protein functionally and physically associates with the smc5/smc6 protein complex and is required for chromosome integrity and recombination repair in fission yeast.

Morikawa H et al. Mol Cell Biol 2004 Nov;24(21):9401-13

PMID:19819763 - Mms1-Mms22 complex protects genome integrity in Schizosaccharomyces pombe.

Dovey CL et al. DNA Repair (Amst) 2009 Dec 03;8(12):1390-9

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83