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protein coding gene - wtf4 (SPCC548.03c) - wtf meiotic drive antidote Wtf4 OR wtf meiotic drive poison Wtf4

Gene summary

Standard name
wtf4
Systematic ID
SPCC548.03c
Product
wtf meiotic drive antidote Wtf4 OR wtf meiotic drive poison Wtf4
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPCC548.03, wts0403a, wtf13
UniProt ID
Q9P3V0
ORFeome ID
51/51D11
Characterisation status
meiotic driver
Feature type
mRNA gene
Genomic location
chromosome III: 221149..222935 reverse strand

Annotation

Comment

PBO:0091895 - heterologously expressed Wtf4 poison is toxic to S. cerevisiae vegetative cells, and rescued by Wtf4 antidote

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GO biological process

GO:0110134 - meiotic drive

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GO cellular component

GO:0072324 - ascus epiplasm

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GO:0005737 - cytoplasm

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GO:0005789 - endoplasmic reticulum membrane

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GO:0000324 - fungal-type vacuole

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GO:0140549 - spore inner membrane

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GO:0005774 - vacuolar membrane

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Modification

MOD:00046 - O-phospho-L-serine

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Protein features

PBO:0111903 - wtf element

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Protein sequence feature

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001489 - inviable vegetative cell

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Genotypes:

Taxonomic conservation

PBO:0016878 - Schizosaccharomyces pombe specific

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF03303WTFWTFPFAM
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

References / Literature

PMID:33108274 - The wtf4 meiotic driver utilizes controlled protein aggregation to generate selective cell death.
Nuckolls NL et al. Elife 2020 Oct 27;9
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:34895466 - Diverse mating phenotypes impact the spread of wtf meiotic drivers in Schizosaccharomyces pombe .
López Hernández JF et al. Elife 2021 Dec 13;10
PMID:38097609 - Ubiquitination-mediated Golgi-to-endosome sorting determines the toxin-antidote duality of fission yeast wtf meiotic drivers.
Zheng JX et al. Nat Commun 2023 Dec 14;14(1):8334
PMID:32032353 - Dramatically diverse Schizosaccharomyces pombe wtf meiotic drivers all display high gamete-killing efficiency.
Bravo Núñez MA et al. PLoS Genet 2020 Feb;16(2):e1008350
PMID:12952871 - Retrotransposons and their recognition of pol II promoters: a comprehensive survey of the transposable elements from the complete genome sequence of Schizosaccharomyces pombe.
Bowen NJ et al. Genome Res 2003 Sep;13(9):1984-97
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:29084823 - Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast.
Navarro FJ et al. J Cell Sci 2017 Dec 15;130(24):4144-4154
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:28631610 - A large gene family in fission yeast encodes spore killers that subvert Mendel's law.
Hu W et al. Elife 2017 Jun 20;6
PMID:30991417 - Killer Meiotic Drive and Dynamic Evolution of the wtf Gene Family.
Eickbush MT et al. Mol Biol Evol 2019 Jun 01;36(6):1201-1214
PMID:28631612 - wtf genes are prolific dual poison-antidote meiotic drivers.
Nuckolls NL et al. Elife 2017 Jun 20;6
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7