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protein coding gene - nse1 (SPCC550.05) - Smc5-6 complex ubiquitin-protein ligase E3 subunit Nse1

Gene summary

Standard name
nse1
Systematic ID
SPCC550.05
Product
Smc5-6 complex ubiquitin-protein ligase E3 subunit Nse1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q53EK2
ORFeome ID
06/06H04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1193987..1196023 forward strand

Annotation

GO biological process

GO:0006281 - DNA repair

References:

GO:0000724 - double-strand break repair via homologous recombination

References:

GO:0007127 - meiosis I

References:

GO cellular component

GO:0005634 - nucleus

References:

GO:0030915 - Smc5-Smc6 complex

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0061630 - ubiquitin protein ligase activity

References:

Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

Protein features

PBO:0111749 - ubiquitin-protein ligase E3

References:

PBO:0111746 - zf-C3HC4 type (RING finger)

References:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000671 - abnormal rDNA separation

References:

Genotypes:

FYPO:0000913 - abnormal sporulation resulting in formation of ascus containing non-uniform spores

References:

Genotypes:

FYPO:0004077 - abnormal sporulation resulting in formation of ascus with single large spore

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0001913 - abolished ubiquitin ligase activity

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0000185 - decreased gene conversion during vegetative growth

References:

Genotypes:

FYPO:0002485 - decreased intergenic meiotic recombination

References:

Genotypes:

FYPO:0007272 - decreased protein localization to nucleoplasm during cellular response to DNA damage

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0000584 - decreased sporulation frequency

References:

Genotypes:

FYPO:0002262 - inviable after spore germination, multiple cell divisions, elongated cell

References:

Genotypes:

FYPO:0004197 - inviable elongated cell with fragmented nucleus and mitotic cell cycle arrest in interphase

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

References:

Genotypes:

FYPO:0004329 - normal mitotic rDNA separation

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000267 - sensitive to ionizing radiation during vegetative growth

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF07574SMC_Nse1Nse1Pfam
cd16493RING-CH-C4HC3_NSE1Nse1_RING_C4HC3-typeCDD
G3DSA:1.10.10.10:FF:000270CATH-FunFam
G3DSA:1.10.10.10WH-like_DNA-bd_sfCATH-Gene3D
G3DSA:3.90.1150.220CATH-Gene3D
PTHR20973Nse1PANTHER

Orthologs

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26446992 - Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA.
Zabrady K et al. Nucleic Acids Res 2016 Feb 18;44(3):1064-79
PMID:32546830 - A role of the Nse4 kleisin and Nse1/Nse3 KITE subunits in the ATPase cycle of SMC5/6.
Vondrova L et al. Sci Rep 2020 Jun 16;10(1):9694
PMID:16478984 - The Nse5-Nse6 dimer mediates DNA repair roles of the Smc5-Smc6 complex.
Pebernard S et al. Mol Cell Biol 2006 Mar;26(5):1617-30
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:15601840 - Composition and architecture of the Schizosaccharomyces pombe Rad18 (Smc5-6) complex.
Sergeant J et al. Mol Cell Biol 2005 Jan;25(1):172-84
PMID:25002536 - A novel histone deacetylase complex in the control of transcription and genome stability.
Zilio N et al. Mol Cell Biol 2014 Sep 15;34(18):3500-14
PMID:15331764 - Nse1, Nse2, and a novel subunit of the Smc5-Smc6 complex, Nse3, play a crucial role in meiosis.
Pebernard S et al. Mol Biol Cell 2004 Nov;15(11):4866-76
PMID:18667531 - Nse1 RING-like domain supports functions of the Smc5-Smc6 holocomplex in genome stability.
Pebernard S et al. Mol Biol Cell 2008 Oct;19(10):4099-109
PMID:14701739 - Coordination of DNA damage responses via the Smc5/Smc6 complex.
Harvey SH et al. Mol Cell Biol 2004 Jan;24(2):662-74
PMID:12966087 - Novel essential DNA repair proteins Nse1 and Nse2 are subunits of the fission yeast Smc5-Smc6 complex.
McDonald WH et al. J Biol Chem 2003 Nov 14;278(46):45460-7
PMID:21364888 - Interactions between the Nse3 and Nse4 components of the SMC5-6 complex identify evolutionarily conserved interactions between MAGE and EID Families.
Hudson JJ et al. PLoS One 2011 Feb 25;6(2):e17270
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:24687850 - H2A.Z-dependent regulation of cohesin dynamics on chromosome arms.
Tapia-Alveal C et al. Mol Cell Biol 2014 Jun;34(11):2092-104
PMID:35011726 - Role of Nse1 Subunit of SMC5/6 Complex as a Ubiquitin Ligase.
Kolesar P et al. Cells 2022 Jan 04;11(1)
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83