protein coding gene - qns1 (SPCC553.02) - glutamine-dependent NAD(+) synthetase Qns1

Gene summary

Standard name: qns1
Systematic ID: SPCC553.02
Product: glutamine-dependent NAD(+) synthetase Qns1
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: O74940
ORFeome ID: 29/29A04
Characterisation status: biological role published
Feature type: mRNA gene
Genomic location: chromosome III: 305618..308619 reverse strand

Annotation

PBO:0005079 - 3.5.-.-

PBO:0005078 - 6.3.5.1

Disease association

MONDO:0030077 - vertebral, cardiac, renal, and limb defects syndrome 3

References:

PB_REF:0000006

GO biological process

GO:0009435 - NAD+ biosynthetic process

References:

PMID:39378339

GO cellular component

GO:0005829 - cytosol

References:

PMID:16823372

GO:0005634 - nucleus

References:

GO_REF:0000024

GO molecular function

GO:0005524 - ATP binding

References:

GO_REF:0000002

GO:0004359 - glutaminase activity

References:

GO_REF:0000033

GO:0003952 - NAD+ synthase (glutamine-hydrolyzing) activity

References:

PMID:39378339

Modification

MOD:00046 - O-phospho-L-serine

References:

PMID:25720772

MOD:00047 - O-phospho-L-threonine

References:

PMID:25720772

MOD:01148 - ubiquitinylated lysine

References:

PMID:37970674

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PMID:39705284

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0008446 - increased cellular nicotinamide level

References:

PMID:39378339

Genotypes:

qns1+(wild type)[Knockdown]

FYPO:0008444 - increased cellular nicotinic acid adenine dinucleotide level

References:

PMID:39378339

Genotypes:

qns1+(wild type)[Knockdown]

FYPO:0008445 - increased cellular nicotinic acid level

References:

PMID:39378339

Genotypes:

qns1+(wild type)[Knockdown]

FYPO:0002107 - inviable stubby vegetative cell

References:

PMID:23697806

Genotypes:

qns1delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

qns1delta

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF02540	NAD_synthase	NAD/GMP_synthase	PFAM
PF00795	CN_hydrolase	C-N_Hydrolase	PFAM
cd00553	NAD_synthase	NAD_synthase	CDD
cd07570	GAT_Gln-NAD-synth		CDD
PS50263	CN_HYDROLASE	C-N_Hydrolase	PROSITE_PROFILES
G3DSA:3.40.50.620:FF:000036			FUNFAM
G3DSA:3.60.110.10:FF:000003			FUNFAM
SSF52402	Adenine nucleotide alpha hydrolases-like		SUPERFAMILY
SSF56317	Carbon-nitrogen hydrolase	C-N_Hydrolase_sf	SUPERFAMILY
G3DSA:3.40.50.620	HUPs	Rossmann-like_a/b/a_fold	GENE3D
G3DSA:3.60.110.10		C-N_Hydrolase_sf	GENE3D
PTHR23090	NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE	NAD_synthase	PANTHER
PIRSF006630	NADS_GAT	Gln-dep_NAD_synthase	PIRSF
MF_02090	NadE_glutamine_dep	Gln-dep_NAD_synthase	HAMAP
TIGR00552	nadE	NAD_synthase	NCBIFAM

Orthologs

NADSYN1 (HGNC:29832) Homo sapiens
qns1 (SJAG_02618) Schizosaccharomyces japonicus
QNS1 (YHR074W) Saccharomyces cerevisiae

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.: Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.: Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.: Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:39378339 - Arrayed CRISPRi library to suppress genes required for Schizosaccharomyces pombe viability.: Ishikawa K et al. J Cell Biol 2025 Jan 06;224(1)
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.: Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.: Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
GO_REF:0000002 - Comments
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36