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protein coding gene - qns1 (SPCC553.02) - glutamine-dependent NAD(+) synthetase Qns1

Gene summary

Standard name
qns1
Systematic ID
SPCC553.02
Product
glutamine-dependent NAD(+) synthetase Qns1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O74940
ORFeome ID
29/29A04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 305618..308619 reverse strand

Annotation

PBO:0005079 - 3.5.-.-

PBO:0005078 - 6.3.5.1

Disease association

MONDO:0030077 - vertebral, cardiac, renal, and limb defects syndrome 3

References:

GO biological process

GO:0034355 - NAD+ biosynthetic process via the salvage pathway

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

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GO molecular function

GO:0005524 - ATP binding

References:

GO:0004359 - glutaminase activity

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GO:0003952 - NAD+ synthase (glutamine-hydrolyzing) activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:01148 - ubiquitinylated lysine

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0008446 - increased cellular nicotinamide level

References:

Genotypes:

FYPO:0008444 - increased cellular nicotinic acid adenine dinucleotide level

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Genotypes:

FYPO:0008445 - increased cellular nicotinic acid level

References:

Genotypes:

FYPO:0002107 - inviable stubby vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00795CN_hydrolaseC-N_HydrolasePfam
PF02540NAD_synthaseNAD/GMP_synthasePfam
cd00553NAD_synthaseNAD_synthaseCDD
cd07570GAT_Gln-NAD-synthCDD
PS50263CN_HYDROLASEC-N_HydrolasePROSITE profiles
G3DSA:3.40.50.620:FF:000036CATH-FunFam
G3DSA:3.60.110.10:FF:000003CATH-FunFam
G3DSA:3.40.50.620Rossmann-like_a/b/a_foldCATH-Gene3D
G3DSA:3.60.110.10C-N_Hydrolase_sfCATH-Gene3D
SSF52402SUPERFAMILY
SSF56317C-N_Hydrolase_sfSUPERFAMILY
PTHR23090NAD_synthasePANTHER
PIRSF006630NADS_GATGln-dep_NAD_synthasePIRSF
TIGR00552nadENAD_synthaseNCBIFAM
MF_02090NadE_glutamine_depGln-dep_NAD_synthaseHAMAP

Orthologs

References / Literature

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:39378339 - Arrayed CRISPRi library to suppress genes required for Schizosaccharomyces pombe viability.
Ishikawa K et al. J Cell Biol 2025 Jan 06;224(1)
GO_REF:0000002 - Comments
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83