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protein coding gene - kpa1 (SPCC553.07c) - DinB translesion DNA repair polymerase, pol kappa

Gene summary

Standard name
kpa1
Systematic ID
SPCC553.07c
Product
DinB translesion DNA repair polymerase, pol kappa
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
mug40, pol(kappa), pol-kappa, dinb
UniProt ID
O74944
ORFeome ID
45/45B06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 293376..295247 forward strand

Annotation

PBO:0000444 - 2.7.7.7

GO biological process

GO:0006974 - DNA damage response

References:

GO:0006281 - DNA repair

References:

GO:0070987 - error-free translesion synthesis

References:

GO:0042276 - error-prone translesion synthesis

References:

GO:0019985 - translesion synthesis

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0003684 - damaged DNA binding

References:

GO:0003887 - DNA-directed DNA polymerase activity

References:

Modification

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0005327 - abolished error-free translesion synthesis of cyclobutane pyrimidine dimers

References:

Genotypes:

FYPO:0005324 - decreased error-free translesion synthesis of cyclobutane pyrimidine dimers

References:

Genotypes:

FYPO:0005330 - decreased error-free translesion synthesis of pyrimidine-pyrimidone 6-4 photoproducts

References:

Genotypes:

FYPO:0005331 - decreased error-prone translesion synthesis of pyrimidine-pyrimidone 6-4 photoproducts

References:

Genotypes:

FYPO:0003085 - decreased replication fork arrest at mating-type locus

References:

Genotypes:

FYPO:0005357 - decreased site-specific DNA replication termination at RTS1 barrier

References:

Genotypes:

FYPO:0005361 - increased level of large-Y replication intermediates

References:

Genotypes:

FYPO:0000256 - mutator

References:

Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

References:

Genotypes:

FYPO:0005775 - sensitive to acetaldehyde

References:

Genotypes:

FYPO:0000102 - sensitive to cisplatin

References:

Genotypes:

FYPO:0007330 - sensitive to formaldehyde

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0005324 - decreased error-free translesion synthesis of cyclobutane pyrimidine dimers

References:

Genotypes:

FYPO:0005331 - decreased error-prone translesion synthesis of pyrimidine-pyrimidone 6-4 photoproducts

References:

Genotypes:

FYPO:0003445 - increased duration of mitotic DNA damage checkpoint during cellular response to UV

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0005329 - normal error-free translesion synthesis of pyrimidine-pyrimidone 6-4 photoproducts

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

References:

Genotypes:

FYPO:0009031 - resistance to bleomycin

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0007330 - sensitive to formaldehyde

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

Protein features

IDNameInterPro nameDB name
PF00817IMSUmuCPFAM
PF11799IMS_CDNA_pol_Y-fam_little_fingerPFAM
cd03586PolY_Pol_IV_kappaDNApol_IVCDD
PS50173UMUCUmuCPROSITE_PROFILES
PS51908ZF_UBZ4Rad18_UBZ4PROSITE_PROFILES
G3DSA:3.40.1170.60:FF:000012FUNFAM
G3DSA:3.30.1490.100:FF:000004FUNFAM
G3DSA:1.10.150.810:FF:000004FUNFAM
SSF100879Lesion bypass DNA polymerase (Y-family), little finger domainDNA_pol_Y-fam_lit_finger_sfSUPERFAMILY
SSF56672DNA/RNA polymerasesDNA/RNA_pol_sfSUPERFAMILY
G3DSA:3.40.1170.60GENE3D
G3DSA:3.30.1490.100DNA_pol_Y-fam_lit_finger_sfGENE3D
G3DSA:1.10.150.810GENE3D
G3DSA:3.30.160.60Classic Zinc FingerGENE3D
G3DSA:3.30.70.270Rev_trsase/Diguanyl_cyclaseGENE3D
PTHR11076DNA REPAIR POLYMERASE UMUC / TRANSFERASE FAMILY MEMBERDNA_polymerase-YPANTHER
CoilCoilCOILS
MF_01113DNApol_IVDNApol_IVHAMAP
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMOBIDB-Negative-Polyelectrolyte
NF002677PRK02406.1NCBIFAM

Orthologs

References / Literature

PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25533348 - Tolerance of deregulated G1/S transcription depends on critical G1/S regulon genes to prevent catastrophic genome instability.
Caetano C et al. Cell Rep 2014 Dec 24;9(6):2279-89
PMID:20404181 - Postreplication gaps at UV lesions are signals for checkpoint activation.
Callegari AJ et al. Proc Natl Acad Sci U S A 2010 May 04;107(18):8219-24
PMID:25293972 - Increased meiotic crossovers and reduced genome stability in absence of Schizosaccharomyces pombe Rad16 (XPF).
Mastro TL et al. Genetics 2014 Dec;198(4):1457-72
PMID:19416828 - Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart.
Inagawa T et al. Proc Natl Acad Sci U S A 2009 May 12;106(19):7927-32
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:27473316 - Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.
Ranatunga NS et al. G3 (Bethesda) 2016 Oct 13;6(10):3049-3063
PMID:17515930 - Rad3-dependent phosphorylation of the checkpoint clamp regulates repair-pathway choice.
Kai M et al. Nat Cell Biol 2007 Jun;9(6):691-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20453833 - Rad8Rad5/Mms2-Ubc13 ubiquitin ligase complex controls translesion synthesis in fission yeast.
Coulon S et al. EMBO J 2010 Jun 16;29(12):2048-58
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
GO_REF:0000002 - Comments
PMID:15972456 - Brc1-mediated DNA repair and damage tolerance.
Sheedy DM et al. Genetics 2005 Oct;171(2):457-68
PMID:27687866 - Genetic controls of DNA damage avoidance in response to acetaldehyde in fission yeast.
Noguchi C et al. Cell Cycle 2017 Jan 02;16(1):45-58
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:12514100 - Checkpoint activation regulates mutagenic translesion synthesis.
Kai M et al. Genes Dev 2003 Jan 01;17(1):64-76
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:32034465 - Genetic investigation of formaldehyde-induced DNA damage response in Schizosaccharomyces pombe.
Anandarajan V et al. Curr Genet 2020 Jun;66(3):593-605
PMID:19714215 - The fission yeast homeodomain protein Yox1p binds to MBF and confines MBF-dependent cell-cycle transcription to G1-S via negative feedback.
Aligianni S et al. PLoS Genet 2009 Aug;5(8):e1000626
PMID:34228709 - Expression of the cancer-associated DNA polymerase ε P286R in fission yeast leads to translesion synthesis polymerase dependent hypermutation and defective DNA replication.
Soriano I et al. PLoS Genet 2021 Jul;17(7):e1009526
PMID:17277362 - Brc1-mediated rescue of Smc5/6 deficiency: requirement for multiple nucleases and a novel Rad18 function.
Lee KM et al. Genetics 2007 Apr;175(4):1585-95
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323