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protein coding gene - spb70 (SPCC553.09c) - DNA polymerase alpha B-subunit

Gene summary

Standard name
spb70
Systematic ID
SPCC553.09c
Product
DNA polymerase alpha B-subunit
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
pol12
UniProt ID
O74946
ORFeome ID
36/36H01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 287229..289704 forward strand

Annotation

GO biological process

GO:0006269 - DNA replication, synthesis of primer

References:

GO:1902975 - mitotic DNA replication initiation

References:

GO cellular component

GO:0005658 - alpha DNA polymerase:primase complex

References:

GO:0000785 - chromatin

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0003677 - DNA binding

References:

Multi-locus phenotype

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000611 - abnormal cell cycle arrest in mitotic S phase

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0005788 - increased gene conversion during vegetative growth

References:

Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

References:

Genotypes:

FYPO:0000839 - inviable elongated mononucleate aseptate cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF22062OB_DPOA2OB_DPOA2PFAM
PF04042DNA_pol_E_BDNA_pol_a/d/e_bsuPFAM
G3DSA:3.60.21.60GENE3D
PTHR23061DNA POLYMERASE 2 ALPHA 70 KDA SUBUNITDNA_pol_alpha_bsuPANTHER
PIRSF018300DNA_pol_alpha_2DNA_pol_alpha_bsuPIRSF

Orthologs

References / Literature

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:34108240 - The intra-S phase checkpoint directly regulates replication elongation to preserve the integrity of stalled replisomes.
Liu Y et al. Proc Natl Acad Sci U S A 2021 Jun 15;118(24)
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
GO_REF:0000002 - Comments
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:29084823 - Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast.
Navarro FJ et al. J Cell Sci 2017 Dec 15;130(24):4144-4154
PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.
McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238
PMID:32355220 - DNA replication machinery prevents Rad52-dependent single-strand annealing that leads to gross chromosomal rearrangements at centromeres.
Onaka AT et al. Commun Biol 2020 Apr 30;3(1):202
PMID:15314153 - The B-subunit of DNA polymerase alpha-primase associates with the origin recognition complex for initiation of DNA replication.
Uchiyama M et al. Mol Cell Biol 2004 Sep;24(17):7419-34
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36