protein coding gene - toa2 (SPCC553.11c) - transcription factor TFIIA complex small subunit Toa2

Gene summary

Standard name: toa2
Systematic ID: SPCC553.11c
Product: transcription factor TFIIA complex small subunit Toa2
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: O74948
ORFeome ID: 05/05A06
Characterisation status: biological role inferred
Feature type: mRNA gene
Genomic location: chromosome III: 281854..283213 forward strand

Annotation

GO biological process

GO:0051123 - RNA polymerase II preinitiation complex assembly

References:

GO_REF:0000033

GO cellular component

GO:0005829 - cytosol

References:

PMID:16823372

GO:0005634 - nucleus

References:

PMID:16823372

GO:0005672 - transcription factor TFIIA complex

References:

GO_REF:0000033

GO molecular function

GO:0016251 - RNA polymerase II general transcription initiation factor activity

References:

GO_REF:0000024

GO:0017025 - TBP-class protein binding

References:

GO_REF:0000033

Modification

MOD:00006 - N-glycosylated residue

References:

PMID:22633491

MOD:01148 - ubiquitinylated lysine

References:

PMID:37970674

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

toa2delta

FYPO:0002451 - inviable vegetative cell with abnormal cell shape

References:

PMID:23697806

Genotypes:

toa2delta

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

PMID:21511999

Protein features

ID	Name	InterPro name	DB name
PF02268	TFIIA_gamma_N	TFIIA_gsu_N	PFAM
PF02751	TFIIA_gamma_C	TFIIA_gsu_C	PFAM
cd10145	TFIIA_gamma_N	TFIIA_gsu_N	CDD
cd10014	TFIIA_gamma_C	TFIIA_gsu_C	CDD
G3DSA:1.10.287.190:FF:000001			FUNFAM
G3DSA:2.30.18.10:FF:000003			FUNFAM
SSF47396	Transcription factor IIA (TFIIA), alpha-helical domain		SUPERFAMILY
SSF50784	Transcription factor IIA (TFIIA), beta-barrel domain	TFIIA_b-brl	SUPERFAMILY
G3DSA:2.30.18.10		TFIIA_b-brl	GENE3D
G3DSA:1.10.287.190		TFIIA_a-hlx	GENE3D
PTHR10966	TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2	TFIIA_gsu	PANTHER
PIRSF009415	TFIIA_gamma_hum	TFIIA_gsu	PIRSF

Orthologs

GTF2A2 (HGNC:4647) Homo sapiens
toa2 (SJAG_02174) Schizosaccharomyces japonicus
TOA2 (YKL058W) Saccharomyces cerevisiae

References / Literature

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.: Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28060464 - RNA polymerase II components and Rrn7 form a preinitiation complex on the HomolD box to promote ribosomal protein gene expression in Schizosaccharomyces pombe.: Montes M et al. FEBS J 2017 Feb;284(4):615-633
PMID:22540037 - Predicting the fission yeast protein interaction network.: Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000033 - Annotation inferences using phylogenetic trees
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.: Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.: Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6