protein coding gene - cut1 (SPCC5E4.04) - separase/separin
Gene summary
- Standard name
- cut1
- Systematic ID
- SPCC5E4.04
- Product
- separase/separin
- Organism
- Schizosaccharomyces pombe
(fission yeast)
- Synonyms
- ptr4
- UniProt ID
- P18296
- ORFeome ID
- 31/31B09
- Characterisation status
- biological role published
- Feature type
- mRNA gene
- Genomic location
- chromosome III: 643409..649578 forward strand
Annotation
Quantitative gene expression
Protein features
| ID | Name | InterPro name | DB name |
| PF03568 | Separin_C | | Pfam |
| PS51700 | SEPARIN | SEPARIN_core_dom | PROSITE profiles |
| PTHR12792 | | Peptidase_C50 | PANTHER |
Orthologs
- ESPL1 (HGNC:16856) Homo sapiens
- cut1 (SJAG_02542) Schizosaccharomyces japonicus
- ESP1 (YGR098C) Saccharomyces cerevisiae
References / Literature
- PMID:12653962 - A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids.
- Matsumura T et al. Genes Cells 2003 Apr;8(4):341-55
- PMID:10582241 - Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization.
- Yanagida M et al. Philos Trans R Soc Lond B Biol Sci 1999 Sep 29;354(1389):1559-69; discussion 1569-70
- PMID:10651900 - Cell cycle mechanisms of sister chromatid separation; roles of Cut1/separin and Cut2/securin.
- Yanagida M Genes Cells 2000 Jan;5(1):1-8
- PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
- Hayles J et al. Open Biol 2013 May 22;3(5):130053
- PMID:24687850 - H2A.Z-dependent regulation of cohesin dynamics on chromosome arms.
- Tapia-Alveal C et al. Mol Cell Biol 2014 Jun;34(11):2092-104
- PMID:15161942 - The nucleolus is involved in mRNA export from the nucleus in fission yeast.
- Ideue T et al. J Cell Sci 2004 Jun 15;117(Pt 14):2887-95
- PMID:30072439 - The putative ceramide-conjugation protein Cwh43 regulates G0 quiescence, nutrient metabolism and lipid homeostasis in fission yeast.
- Nakazawa N et al. J Cell Sci 2018 Aug 21;131(16)
- PMID:16861909 - Fission yeast MAP kinase is required for the increased securin-separase interaction that rescues separase mutants under stresses.
- Kawasaki Y et al. Cell Cycle 2006 Aug;5(16):1831-9
- PMID:12390246 - Cut1/separase C-terminus affects spindle pole body positioning in interphase of fission yeast: pointed nuclear formation.
- Nakamura T et al. Genes Cells 2002 Nov;7(11):1113-24
- PMID:22645648 - The reverse, but coordinated, roles of Tor2 (TORC1) and Tor1 (TORC2) kinases for growth, cell cycle and separase-mediated mitosis in Schizosaccharomyces pombe.
- Ikai N et al. Open Biol 2011 Nov;1(3):110007
- PMID:7957061 - Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis.
- Saka Y et al. EMBO J 1994 Oct 17;13(20):4938-52
- PMID:14972679 - Two fission yeast homologs of Drosophila Mei-S332 are required for chromosome segregation during meiosis I and II.
- Rabitsch KP et al. Curr Biol 2004 Feb 17;14(4):287-301
- PMID:15329725 - Separase-mediated cleavage of cohesin at interphase is required for DNA repair.
- Nagao K et al. Nature 2004 Aug 26;430(7003):1044-8
- PMID:2203537 - The fission yeast cut1+ gene regulates spindle pole body duplication and has homology to the budding yeast ESP1 gene.
- Uzawa S et al. Cell 1990 Sep 07;62(5):913-25
- PMID:1934126 - Common genes and pathways in the regulation of the mitotic and meiotic cell cycles of Schizosaccharomyces pombe.
- Grallert B et al. Curr Genet 1991 Aug;20(3):199-204
- PMID:2229195 - Continued DNA synthesis after a mitotic block in the double mutant cut1 cdc11 of the fission yeast Schizosaccharomyces pombe.
- Creanor J et al. J Cell Sci 1990 Jul;96 ( Pt 3):435-8
- PMID:9168469 - Isolation and molecular characterization of mRNA transport mutants in Schizosaccharomyces pombe.
- Azad AK et al. Mol Biol Cell 1997 May;8(5):825-41
- PMID:8395535 - Identification of seven new cut genes involved in Schizosaccharomyces pombe mitosis.
- Samejima I et al. J Cell Sci 1993 May;105 ( Pt 1):135-43
- PMID:9649518 - A screen for genes involved in the anaphase proteolytic pathway identifies tsm1(+), a novel Schizosaccharomyces pombe gene important for microtubule integrity.
- Grishchuk EL et al. Genetics 1998 Jul;149(3):1251-64
- PMID:9802907 - Dynamics of centromeres during metaphase-anaphase transition in fission yeast: Dis1 is implicated in force balance in metaphase bipolar spindle.
- Nabeshima K et al. Mol Biol Cell 1998 Nov;9(11):3211-25
- PMID:9635190 - Cut1 is loaded onto the spindle by binding to Cut2 and promotes anaphase spindle movement upon Cut2 proteolysis.
- Kumada K et al. Curr Biol 1998 May 21;8(11):633-41
- PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.
- Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82
- PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
- Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
- PMID:16483313 - Securin can have a separase cleavage site by substitution mutations in the domain required for stabilization and inhibition of separase.
- Nagao K et al. Genes Cells 2006 Mar;11(3):247-60
- PMID:17032733 - S. pombe FEAR protein orthologs are not required for release of Clp1/Flp1 phosphatase from the nucleolus during mitosis.
- Chen CT et al. J Cell Sci 2006 Nov 01;119(Pt 21):4462-6
- PMID:14511667 - A mutation in the gene involved in sister chromatid separation causes a defect in nuclear mRNA export in fission yeast.
- Kalam Azad A et al. Biochem Biophys Res Commun 2003 Oct 10;310(1):176-81
- PMID:16453724 - Isolation and characterization of Schizosaccharomyces pombe cutmutants that block nuclear division but not cytokinesis.
- Hirano T et al. EMBO J 1986 Nov;5(11):2973-9
- PMID:41786187 - Shugoshin holds the potential to inhibit APC/C and thereby prevents separase activation.
- Zhang K et al. Int J Biol Macromol 2026 Mar 03;:151175
- PMID:22084378 - Nutrient limitations alter cell division control and chromosome segregation through growth-related kinases and phosphatases.
- Yanagida M et al. Philos Trans R Soc Lond B Biol Sci 2011 Dec 27;366(1584):3508-20
- PMID:8065367 - Identification of cut8+ and cek1+, a novel protein kinase gene, which complement a fission yeast mutation that blocks anaphase.
- Samejima I et al. Mol Cell Biol 1994 Sep;14(9):6361-71
- PMID:17178839 - Fission yeast Cut8 is required for the repair of DNA double-strand breaks, ribosomal DNA maintenance, and cell survival in the absence of Rqh1 helicase.
- Kearsey SE et al. Mol Cell Biol 2007 Mar;27(5):1558-67
- PMID:35320724 - Cdc48 influence on separase levels is independent of mitosis and suggests translational sensitivity of separase.
- Vijayakumari D et al. Cell Rep 2022 Mar 22;38(12):110554
- PMID:20967237 - Fission yeast cells undergo nuclear division in the absence of spindle microtubules.
- Castagnetti S et al. PLoS Biol 2010 Oct 12;8(10):e1000512
- PMID:8978688 - Fission yeast Cut1 and Cut2 are essential for sister chromatid separation, concentrate along the metaphase spindle and form large complexes.
- Funabiki H et al. EMBO J 1996 Dec 02;15(23):6617-28
- PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
- Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
- PMID:29735656 - Suppressor mutation analysis combined with 3D modeling explains cohesin's capacity to hold and release DNA.
- Xu X et al. Proc Natl Acad Sci U S A 2018 May 22;115(21):E4833-E4842
- PMID:16904908 - Cdc48 is required for the stability of Cut1/separase in mitotic anaphase.
- Ikai N et al. J Struct Biol 2006 Oct;156(1):50-61
- PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
- Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
- PMID:21511999 - Comparative functional genomics of the fission yeasts.
- Rhind N et al. Science 2011 May 20;332(6032):930-6
- PMID:14532136 - Rec8 cleavage by separase is required for meiotic nuclear divisions in fission yeast.
- Kitajima TS et al. EMBO J 2003 Oct 15;22(20):5643-53
- PMID:19528228 - Smc5-Smc6-dependent removal of cohesin from mitotic chromosomes.
- Outwin EA et al. Mol Cell Biol 2009 Aug;29(16):4363-75
- PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
- Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
- PMID:24583014 - Slow checkpoint activation kinetics as a safety device in anaphase.
- Kamenz J et al. Curr Biol 2014 Mar 17;24(6):646-51
- PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
- Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
- PMID:16491466 - Construction of a protease-deficient strain set for the fission yeast Schizosaccharomyces pombe, useful for effective production of protease-sensitive heterologous proteins.
- Idiris A et al. Yeast 2006 Jan 30;23(2):83-99
- PMID:26527280 - Robust Ordering of Anaphase Events by Adaptive Thresholds and Competing Degradation Pathways.
- Kamenz J et al. Mol Cell 2015 Nov 05;60(3):446-59
- PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
- Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
- PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
- Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
- PMID:38780300 - Nitrogen availability is important for preventing catastrophic mitosis in fission yeast.
- Zemlianski V et al. J Cell Sci 2024 May 23;
- GO_REF:0000033 - Annotation inferences using phylogenetic trees
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- PMID:9153313 - Isolation and characterization of the Schizosaccharomyces pombe rhp9 gene: a gene required for the DNA damage checkpoint but not the replication checkpoint.
- Willson J et al. Nucleic Acids Res 1997 Jun 01;25(11):2138-46
- PMID:8388878 - Cell cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast.
- Funabiki H et al. J Cell Biol 1993 Jun;121(5):961-76
