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protein coding gene - spt3 (SPCC61.02) - SAGA complex subunit Spt3

Gene summary

Standard name
spt3
Systematic ID
SPCC61.02
Product
SAGA complex subunit Spt3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O14311
ORFeome ID
12/12H05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1442954..1444600 forward strand

Annotation

Comment

PBO:0000206 - deletion mutant expression profiling

References:

GO biological process

GO:0006357 - regulation of transcription by RNA polymerase II

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GO:0045815 - transcription initiation-coupled chromatin remodeling

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GO cellular component

GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO:0000124 - SAGA complex

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GO molecular function

GO:0003713 - transcription coactivator activity

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Multi-locus phenotype

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

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Genotypes:

Protein features

PBO:0111763 - histone fold

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0004604 - decreased chromatin silencing at subtelomere

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0004742 - normal chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0001098 - sensitive to 4-nitroquinoline N-oxide

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0000099 - sensitive to canavanine

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0001207 - sensitive to raffinose

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Genotypes:

FYPO:0000111 - sensitive to rapamycin

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF02269TFIID-18kDaTFIID_TAF13Pfam
cd22926HFD_SPT3CDD
G3DSA:1.10.20.10:FF:000023CATH-FunFam
G3DSA:1.10.20.10Histone-foldCATH-Gene3D
SSF47113Histone-foldSUPERFAMILY
PTHR11380TFIID_TAF13PANTHER

Orthologs

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25245948 - Tls1 regulates splicing of shelterin components to control telomeric heterochromatin assembly and telomere length.
Wang J et al. Nucleic Acids Res 2014 Oct;42(18):11419-32
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:25795664 - Genetic Interaction Landscape Reveals Critical Requirements for Schizosaccharomyces pombe Brc1 in DNA Damage Response Mutants.
Sánchez A et al. G3 (Bethesda) 2015 Mar 19;5(5):953-62
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:19056896 - The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8.
Helmlinger D et al. Genes Dev 2008 Nov 15;22(22):3184-95
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:30573453 - Anti-silencing factor Epe1 associates with SAGA to regulate transcription within heterochromatin.
Bao K et al. Genes Dev 2019 Jan 01;33(1-2):116-126
PMID:9559549 - Identification and analysis of homologues of Saccharomyces cerevisiae Spt3 suggest conserved functional domains.
Madison JM et al. Yeast 1998 Mar 30;14(5):409-17
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:29079657 - TORC1 and TORC2 converge to regulate the SAGA co-activator in response to nutrient availability.
Laboucarié T et al. EMBO Rep 2017 Dec;18(12):2197-2218
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21642955 - Tra1 has specific regulatory roles, rather than global functions, within the SAGA co-activator complex.
Helmlinger D et al. EMBO J 2011 Jun 03;30(14):2843-52
PMID:35157728 - TOR complex 2 contributes to regulation of gene expression via inhibiting Gcn5 recruitment to subtelomeric and DNA replication stress genes.
Cohen A et al. PLoS Genet 2022 Feb;18(2):e1010061
PMID:34460892 - Transcriptome sequencing and screening of genes related to glucose availability in Schizosaccharomyces pombe by RNA-seq analysis.
Tarhan Ç et al. Genet Mol Biol 2021;44(3):e20200245