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protein coding gene - aah3 (SPCC63.02c) - cell and ascus wall (1->3)-alpha-glucan biosynthesis protein Aah3

Gene summary

Standard name
aah3
Systematic ID
SPCC63.02c
Product
cell and ascus wall (1->3)-alpha-glucan biosynthesis protein Aah3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9Y7S9
ORFeome ID
45/45E11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 833581..836028 reverse strand

Annotation

PBO:0001601 - 3.2.1.1

GO biological process

GO:0070591 - ascospore wall biogenesis

References:

GO:0070600 - fungal-type cell wall (1->3)-alpha-glucan biosynthetic process

References:

GO:0071940 - fungal-type cell wall assembly

References:

GO cellular component

GO:0009897 - external side of plasma membrane

References:

GO:0009277 - fungal-type cell wall

References:

GO:0005886 - plasma membrane

References:

GO molecular function

GO:0004134 - 4-alpha-glucanotransferase activity

References:

Miscellaneous functional group

PBO:0000068 - cell surface glycoprotein

Modification

MOD:00689 - disulfide crosslinked residues

References:

MOD:00693 - glycosylated residue

References:

MOD:00818 - glycosylphosphatidylinositolated residue

References:

MOD:00006 - N-glycosylated residue

References:

MOD:00171 - N-seryl-glycosylphosphatidylinositolethanolamine

References:

Multi-locus phenotype

FYPO:0001971 - abnormal cell separation after cytokinesis resulting in chained cells

References:

Genotypes:

FYPO:0002946 - abnormal cell wall

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0001081 - decreased cell wall alpha-glucan level

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Genotypes:

FYPO:0002265 - decreased cell wall galactomannan level

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0001036 - delaminated cell wall during vegetative growth

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Genotypes:

FYPO:0003133 - galactomannan absent from cell wall

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Genotypes:

FYPO:0004860 - increased cell wall beta-glucan level

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Genotypes:

FYPO:0001035 - increased cell wall thickness during vegetative growth

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0006117 - multiseptate spheroid vegetative cell

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Genotypes:

FYPO:0000673 - normal septum assembly

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0000086 - sensitive to tacrolimus

References:

Genotypes:

FYPO:0004103 - viable spherical vegetative cell

References:

Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

References:

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0003625 - abnormal microtubule cytoskeleton morphology during mitotic interphase

References:

Genotypes:

FYPO:0000121 - abnormal sporulation

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0000252 - increased spontaneous diploidization

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0003795 - normal cell wall alpha-glucan level

References:

Genotypes:

FYPO:0004533 - normal cell wall beta-glucan level

References:

Genotypes:

FYPO:0002343 - normal growth on terbinafine

References:

Genotypes:

FYPO:0001120 - pear-shaped vegetative cell

References:

Genotypes:

FYPO:0004534 - premature ascospore release from ascus

References:

Genotypes:

FYPO:0009079 - resistance to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0002642 - sensitive to amphotericin B

References:

Genotypes:

FYPO:0000093 - sensitive to arsenic

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000098 - sensitive to calcium

References:

Genotypes:

FYPO:0001190 - sensitive to cell wall-degrading enzymes

References:

Genotypes:

FYPO:0002640 - sensitive to clotrimazole

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0002641 - sensitive to micafungin

References:

Genotypes:

FYPO:0000271 - sensitive to salt stress

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

References:

Genotypes:

FYPO:0002903 - viable pear-shaped vegetative cell

References:

Genotypes:

FYPO:0002106 - viable stubby vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002197 - viable vegetative cell with abnormal cell shape

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0000110 - orthologs cannot be distinguished

Protein features

IDNameInterPro nameDB name
PF00128Alpha-amylaseGH13_cat_domPFAM
PF09260A_amylase_dom_CA_amylase_C_domPFAM
cd11319AmyAc_euk_AmyACDD
SM00642aamyGH13_cat_domSMART
G3DSA:2.60.40.1180:FF:000037FUNFAM
G3DSA:3.20.20.80:FF:000120FUNFAM
SSF51011Glycosyl hydrolase domainSUPERFAMILY
SSF51445(Trans)glycosidasesGH_hydrolase_sfSUPERFAMILY
G3DSA:3.20.20.80GlycosidasesGENE3D
G3DSA:2.60.40.1180Glyco_hydro_bGENE3D
PTHR10357ALPHA-AMYLASE FAMILY MEMBERPANTHER
PIRSF001024Alph-amyl_fungA-amylase-likePIRSF

Orthologs

References / Literature

PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PB_REF:0000007 - Prediction of GPI-anchored proteins with pointer neural networks
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39
PMID:12845604 - Genome-wide identification of fungal GPI proteins.
De Groot PW et al. Yeast 2003 Jul 15;20(9):781-96
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:27558664 - Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast.
Guo L et al. G3 (Bethesda) 2016 Oct 13;6(10):3317-3333
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25373780 - A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.
Graml V et al. Dev Cell 2014 Oct 27;31(2):227-239
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000036 - Manual annotations that require more than one source of functional data to support the assignment of the associated GO term
PMID:16751704 - An alpha-amylase homologue, aah3, encodes a GPI-anchored membrane protein required for cell wall integrity and morphogenesis in Schizosaccharomyces pombe.
Morita T et al. Biosci Biotechnol Biochem 2006 Jun;70(6):1454-63
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:22848669 - Studies on the roles of clathrin-mediated membrane trafficking and zinc transporter Cis4 in the transport of GPI-anchored proteins in fission yeast.
Jaiseng W et al. PLoS One 2012;7(7):e41946
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:40668835 - α-glucan remodeling by GH13-domain enzymes shapes fungal cell wall architecture.
Jacob A et al. Proc Natl Acad Sci U S A 2025 Jul 22;122(29):e2505509122
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.
Malecki M et al. Genome Biol 2016 Nov 25;17(1):240
PMID:22252817 - A genomewide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis.
Fang Y et al. Antimicrob Agents Chemother 2012 Apr;56(4):1949-59
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527