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protein coding gene - mok14 (SPCC63.04) - spore wall alpha-1,4-glucan synthase, meiotic Mok14

Gene summary

Standard name
mok14
Systematic ID
SPCC63.04
Product
spore wall alpha-1,4-glucan synthase, meiotic Mok14
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9Y704
ORFeome ID
48/48F12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 839962..844165 forward strand

Annotation

PBO:0002492 - 2.4.1.183

GO biological process

GO:0070591 - ascospore wall biogenesis

References:

GO:0070600 - fungal-type cell wall (1->3)-alpha-glucan biosynthetic process

References:

GO cellular component

GO:0005619 - ascospore wall

References:

GO:0036362 - ascus membrane

References:

GO molecular function

GO:0047657 - alpha-1,3-glucan synthase activity

References:

GO:0033840 - alpha-1,4-glucan glucosyltransferase (NDP-glucose donor) activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0002482 - inviable spheroid vegetative cell

References:

Genotypes:

FYPO:0002161 - normal growth on Calcofluor White

References:

Genotypes:

FYPO:0004892 - normal growth on echinocandin

References:

Genotypes:

FYPO:0001315 - normal vegetative cell morphology

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000015 - RNA absent

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0003797 - normal conjugation frequency

References:

Genotypes:

FYPO:0002161 - normal growth on Calcofluor White

References:

Genotypes:

FYPO:0004892 - normal growth on echinocandin

References:

Genotypes:

FYPO:0000590 - normal sporulation

References:

Genotypes:

FYPO:0001315 - normal vegetative cell morphology

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0005132 - spores sensitive to cell wall-degrading enzymes

References:

Genotypes:

FYPO:0005130 - spores sensitive to heat

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0000055 - no apparent S. cerevisiae ortholog

Protein features

IDNameInterPro nameDB name
PF2612712TM_Mok13Mok11-14/Ags1-like_TMPFAM
PF00534Glycos_transf_1Glyco_trans_1PFAM
PF26775HTH_Mok13HTH_Mok13PFAM
PF08323Glyco_transf_5Starch_synth_cat_domPFAM
cd03791GT5_Glycogen_synthase_DULL1-likeCDD
G3DSA:3.40.50.2000:FF:000052FUNFAM
SSF53756UDP-Glycosyltransferase/glycogen phosphorylaseSUPERFAMILY
G3DSA:3.40.50.2000Glycogen Phosphorylase B;GENE3D
PTHR47182CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1-RELATEDMok11-14/Ags1-likePANTHER

Orthologs

References / Literature

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:10087262 - Fission yeast alpha-glucan synthase Mok1 requires the actin cytoskeleton to localize the sites of growth and plays an essential role in cell morphogenesis downstream of protein kinase C function.
Katayama S et al. J Cell Biol 1999 Mar 22;144(6):1173-86
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
GO_REF:0000002 - Comments
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:16420355 - Synthesis of alpha-glucans in fission yeast spores is carried out by three alpha-glucan synthase paralogues, Mok12p, Mok13p and Mok14p.
García I et al. Mol Microbiol 2006 Feb;59(3):836-53
GO_REF:0000116 - Automatic Gene Ontology annotation based on Rhea mapping.
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:20531409 - Global coordination of transcriptional control and mRNA decay during cellular differentiation.
Amorim MJ et al. Mol Syst Biol 2010 Jun 08;6:380