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protein coding gene - prp28 (SPCC63.11) - U5 snRNP-associated ATP-dependent RNA helicase Prp28

Gene summary

Standard name
prp28
Systematic ID
SPCC63.11
Product
U5 snRNP-associated ATP-dependent RNA helicase Prp28
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9Y7T7
ORFeome ID
27/27C08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 857017..859292 forward strand

Annotation

GO biological process

GO:0000354 - cis assembly of pre-catalytic spliceosome

References:

GO cellular component

GO:0071013 - catalytic step 2 spliceosome

References:

GO:0005634 - nucleus

References:

GO:0005682 - U5 snRNP

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0003729 - mRNA binding

References:

GO:0003724 - RNA helicase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

Protein features

PBO:0111747 - DEAD/DEAH box helicase

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0002379 - inviable after spore germination, without cell division, with elongated germ tube

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00270DEADDEAD/DEAH_box_helicase_domPfam
PF00271Helicase_CHelicase_C-likePfam
PF25430DDX23PRP28/DDX23-like_helicalPfam
cd17945DEADc_DDX23CDD
cd18787SF2_C_DEADCDD
PS00039DEAD_ATP_HELICASERNA-helicase_DEAD-box_CSPROSITE patterns
PS51192HELICASE_ATP_BIND_1Helicase_ATP-bdPROSITE profiles
PS51194HELICASE_CTERHelicase_C-likePROSITE profiles
PS51195Q_MOTIFRNA_helicase_DEAD_Q_motifPROSITE profiles
SM00487DEXDcHelicase_ATP-bdSMART
SM00490HELICcHelicase_C-likeSMART
G3DSA:3.40.50.300:FF:000322CATH-FunFam
G3DSA:3.40.50.300P-loop_NTPaseCATH-Gene3D
SSF52540P-loop_NTPaseSUPERFAMILY
PTHR47958PANTHER
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity

Orthologs

References / Literature

PMID:25245948 - Tls1 regulates splicing of shelterin components to control telomeric heterochromatin assembly and telomere length.
Wang J et al. Nucleic Acids Res 2014 Oct;42(18):11419-32
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:41201383 - Combinatorial Analyses of Pre-mRNA Splicing-Defective Mutants Reveal Differential Quantitative Requirements for Shelterin in Distinct Telomere Functions.
Takeuchi M et al. Genes Cells 2025 Nov;30(6):e70064
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
GO_REF:0000002 - Comments
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:28446597 - The histone variant H2A.Z promotes splicing of weak introns.
Nissen KE et al. Genes Dev 2017 Apr 01;31(7):688-701
PMID:27350684 - Mutant allele of rna14 in fission yeast affects pre-mRNA splicing.
Yadav S et al. J Genet 2016 Jun;95(2):389-97
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053