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protein coding gene - isa1 (SPCC645.03c) - mitochondrial [4Fe-4S] cluster assembly and transfer protein Isa1

Gene summary

Standard name
isa1
Systematic ID
SPCC645.03c
Product
mitochondrial [4Fe-4S] cluster assembly and transfer protein Isa1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P78859
ORFeome ID
42/42G08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1230674..1231856 reverse strand

Annotation

Comment

PBO:0003051 - promoter contains CCAAT element

References:

Disease association

MONDO:0033282 - multiple mitochondrial dysfunctions syndrome 5

References:

GO biological process

GO:0044572 - [4Fe-4S] cluster assembly

References:

GO:0016226 - iron-sulfur cluster assembly

References:

GO:0051604 - protein maturation

References:

GO cellular component

GO:0120510 - mitochondrial [4Fe-4S] assembly complex

References:

GO:0005739 - mitochondrion

References:

GO molecular function

GO:0051537 - 2 iron, 2 sulfur cluster binding

References:

GO:0051536 - iron-sulfur cluster binding

References:

GO:0036455 - iron-sulfur transferase activity

References:

GO:0005515 - protein binding

References:

Modification

MOD:00739 - iron-sulfur cluster containing modification

References:

Multi-locus phenotype

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001736 - decreased iron-sulfur cluster transfer

References:

Genotypes:

FYPO:0001711 - destabilized iron-sulfur cluster binding

References:

Genotypes:

FYPO:0002111 - inviable tapered vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01521Fe-S_biosynATAP_core_domPFAM
PS01152HESBFeS_cluster_insertion_CSPROSITE_PATTERNS
G3DSA:2.60.300.12:FF:000001FUNFAM
SSF89360HesB-like domainHesB-like_dom_sfSUPERFAMILY
G3DSA:2.60.300.12HesB-like_dom_sfGENE3D
PTHR10072IRON-SULFUR CLUSTER ASSEMBLY PROTEINFe-S_cluster_asmbl/transferPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
TIGR00049TIGR00049ATAPNCBIFAM

Orthologs

References / Literature

PMID:34660592 - Biogenesis of Iron-Sulfur Clusters and Their Role in DNA Metabolism.
Shi R et al. Front Cell Dev Biol 2021;9:735678
PMID:20085751 - Monothiol glutaredoxin Grx5 interacts with Fe-S scaffold proteins Isa1 and Isa2 and supports Fe-S assembly and DNA integrity in mitochondria of fission yeast.
Kim KD et al. Biochem Biophys Res Commun 2010 Feb 12;392(3):467-72
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:12741836 - Iron-sulfur cluster biosynthesis. A comparative kinetic analysis of native and Cys-substituted ISA-mediated [2Fe-2S]2+ cluster transfer to an apoferredoxin target.
Wu SP et al. Biochemistry 2003 May 20;42(19):5784-91
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:19502236 - Both Php4 function and subcellular localization are regulated by iron via a multistep mechanism involving the glutaredoxin Grx4 and the exportin Crm1.
Mercier A et al. J Biol Chem 2009 Jul 24;284(30):20249-62
PMID:20435771 - Iron-dependent remodeling of fungal metabolic pathways associated with ferrichrome biosynthesis.
Mercier A et al. Appl Environ Microbiol 2010 Jun;76(12):3806-17
PMID:37156397 - Identification of novel coenzyme Q 10 biosynthetic proteins Coq11 and Coq12 in Schizosaccharomyces pombe.
Nishida I et al. J Biol Chem 2023 Jun;299(6):104797
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:20531409 - Global coordination of transcriptional control and mRNA decay during cellular differentiation.
Amorim MJ et al. Mol Syst Biol 2010 Jun 08;6:380
PMID:23115244 - Cells lacking pfh1, a fission yeast homolog of mammalian frataxin protein, display constitutive activation of the iron starvation response.
Gabrielli N et al. J Biol Chem 2012 Dec 14;287(51):43042-51
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:18223116 - Key function for the CCAAT-binding factor Php4 to regulate gene expression in response to iron deficiency in fission yeast.
Mercier A et al. Eukaryot Cell 2008 Mar;7(3):493-508
PMID:16963626 - A transcription factor cascade involving Fep1 and the CCAAT-binding factor Php4 regulates gene expression in response to iron deficiency in the fission yeast Schizosaccharomyces pombe.
Mercier A et al. Eukaryot Cell 2006 Nov;5(11):1866-81
PMID:27590019 - Glutathione-complexed [2Fe-2S] clusters function in Fe-S cluster storage and trafficking.
Fidai I et al. J Biol Inorg Chem 2016 Oct;21(7):887-901
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25347204 - Formation of [4Fe-4S] clusters in the mitochondrial iron-sulfur cluster assembly machinery.
Brancaccio D et al. J Am Chem Soc 2014 Nov 19;136(46):16240-50
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:25806539 - A cascade of iron-containing proteins governs the genetic iron starvation response to promote iron uptake and inhibit iron storage in fission yeast.
Encinar del Dedo J et al. PLoS Genet 2015 Mar;11(3):e1005106
PMID:11941510 - Iron-sulfur cluster biosynthesis: characterization of Schizosaccharomyces pombe Isa1.
Wu G et al. J Biol Inorg Chem 2002 Apr;7(4-5):526-32
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053