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protein coding gene - rgf1 (SPCC645.07) - RhoGEF for Rho1, Rgf1

Gene summary

Standard name
rgf1
Systematic ID
SPCC645.07
Product
RhoGEF for Rho1, Rgf1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9Y7U6
ORFeome ID
30/30E12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1245186..1249470 forward strand

Annotation

GO biological process

GO:0051523 - cell growth mode switching, monopolar to bipolar

References:

GO:0030866 - cortical actin cytoskeleton organization

References:

GO:0061245 - establishment or maintenance of bipolar cell polarity

References:

GO:0009272 - fungal-type cell wall biogenesis

References:

GO:1903139 - positive regulation of cell integrity MAPK cascade

References:

GO:0090334 - regulation of cell wall (1->3)-beta-D-glucan biosynthetic process

References:

GO:1903338 - regulation of cell wall organization or biogenesis

References:

GO cellular component

GO:0051285 - cell cortex of cell tip

References:

GO:1902716 - cell cortex of growing cell tip

References:

GO:0140472 - cell cortex of non-growing cell tip

References:

GO:0032153 - cell division site

References:

GO:0051286 - cell tip

References:

GO:0000935 - division septum

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005085 - guanyl-nucleotide exchange factor activity

References:

GO:0070273 - phosphatidylinositol-4-phosphate binding

References:

GO:0005515 - protein binding

References:

Genome organisation

PBO:0091283 - divergently oriented to functionally related gene

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0003440 - cell lysis during cytokinesis

References:

Genotypes:

FYPO:0003535 - decreased bipolar index

References:

Genotypes:

FYPO:0005798 - decreased protein localization to cell cortex of cell tip during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000255 - increased nuclear protein level during vegetative growth

References:

Genotypes:

FYPO:0007910 - increased vegetative cell shrinkage

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001883 - normal growth on caspofungin

References:

Genotypes:

FYPO:0006008 - normal onset of protein localization to cell division site

References:

Genotypes:

FYPO:0000776 - normal protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0005152 - resistance to caspofungin

References:

Genotypes:

FYPO:0000079 - sensitive to caspofungin

References:

Genotypes:

FYPO:0000105 - sensitive to cyclosporin A

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0002848 - T-shaped cell during recovery from stationary phase

References:

Genotypes:

FYPO:0000013 - T-shaped vegetative cell with normal cell length

References:

Genotypes:

FYPO:0002380 - viable spheroid vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111759 - pleckstrin homology domain

Protein sequence feature

SO:0001531 - nuclear_export_signal

References:

SO:0001528 - nuclear_localization_signal

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000801 - abnormal actin cytoskeleton organization during vegetative growth

References:

Genotypes:

FYPO:0006745 - abnormal cell wall thickness during vegetative growth

References:

Genotypes:

FYPO:0000031 - abnormal mating

References:

Genotypes:

FYPO:0005189 - abnormal re-entry into mitotic cell cycle after arrest in response to hydroxyurea

References:

Genotypes:

FYPO:0005503 - abnormally monopolar protein localization to cell tip

References:

Genotypes:

FYPO:0001018 - abolished NETO

References:

Genotypes:

FYPO:0004188 - abolished protein localization to nucleus during cellular response to hydroxyurea

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0003535 - decreased bipolar index

References:

Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0007182 - decreased cytoplasmic microtubule depolymerization at plus end at cell tip

References:

Genotypes:

FYPO:0001529 - decreased GTP binding

References:

Genotypes:

FYPO:0007450 - decreased maintenance of protein location in cell cortex of cell tip

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0006776 - decreased phosphatidylinositol-4-phosphate binding

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0000929 - decreased protein localization to cell cortex during vegetative growth

References:

Genotypes:

FYPO:0005798 - decreased protein localization to cell cortex of cell tip during vegetative growth

References:

Genotypes:

FYPO:0003208 - decreased protein localization to cell tip, with protein distributed in plasma membrane or cortex

References:

Genotypes:

FYPO:0005643 - decreased protein localization to cell tip, with protein mislocalized to nucleus and spindle pole body

References:

Genotypes:

FYPO:0005338 - decreased protein localization to nucleus during cellular response to hydroxyurea

References:

Genotypes:

FYPO:0002679 - decreased protein phosphorylation

References:

Genotypes:

FYPO:0002376 - decreased protein phosphorylation during cellular response to osmotic stress

References:

Genotypes:

FYPO:0001885 - decreased protein phosphorylation during salt stress

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0003946 - delayed onset of protein localization to cell division site

References:

Genotypes:

FYPO:0001968 - increased 1,3-beta-D-glucan synthase activity

References:

Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0003545 - increased duration of mitotic cell cycle DNA replication checkpoint

References:

Genotypes:

FYPO:0000255 - increased nuclear protein level during vegetative growth

References:

Genotypes:

FYPO:0000972 - increased number of Rad52 foci during vegetative growth

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

References:

Genotypes:

FYPO:0001130 - increased protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0007526 - increased protein phosphorylation during cellular response to salt stress

References:

Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0004511 - long curved interphase microtubules

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0001397 - monopolar actin cortical patch localization to old end

References:

Genotypes:

FYPO:0001366 - normal actin cytoskeleton organization

References:

Genotypes:

FYPO:0001367 - normal cytokinesis

References:

Genotypes:

FYPO:0001883 - normal growth on caspofungin

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0001789 - normal protein localization to nucleus during cellular response to oxidative stress

References:

Genotypes:

FYPO:0008003 - normal protein localization to site of mechanical stress

References:

Genotypes:

FYPO:0004422 - normal protein phosphorylation

References:

Genotypes:

FYPO:0003214 - normal protein phosphorylation during cellular response to glucose starvation

References:

Genotypes:

FYPO:0004154 - normal protein phosphorylation during cellular response to heat

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001473 - resistance to tacrolimus during salt stress

References:

Genotypes:

FYPO:0002642 - sensitive to amphotericin B

References:

Genotypes:

FYPO:0002720 - sensitive to beta-glucanase

References:

Genotypes:

FYPO:0000079 - sensitive to caspofungin

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0002641 - sensitive to micafungin

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0002848 - T-shaped cell during recovery from stationary phase

References:

Genotypes:

FYPO:0000647 - vegetative cell lysis

References:

Genotypes:

FYPO:0001491 - viable vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00610DEPDEP_domPFAM
PF00780CNHCNH_domPFAM
PF15405PH_5PH_5PFAM
PF00621RhoGEFDH_domPFAM
cd00160RhoGEFDH_domCDD
cd04435DEP_fRom2CDD
PS50219CNHCNH_domPROSITE_PROFILES
PS50010DH_2DH_domPROSITE_PROFILES
PS50003PH_DOMAINPH_domainPROSITE_PROFILES
SM00049DEP_3DEP_domSMART
SM00233PH_updatePH_domainSMART
SM00036nik_2CNH_domSMART
SM00325RhoGEF_3DH_domSMART
G3DSA:2.30.29.30:FF:000405FUNFAM
G3DSA:1.20.900.10:FF:000035FUNFAM
SSF50729PH domain-likeSUPERFAMILY
SSF48065DBL homology domain (DH-domain)DBL_dom_sfSUPERFAMILY
SSF46785"Winged helix" DNA-binding domainWH_DNA-bd_sfSUPERFAMILY
G3DSA:1.20.900.10Dbl homology (DH) domainDBL_dom_sfGENE3D
G3DSA:1.10.10.10WH-like_DNA-bd_sfGENE3D
G3DSA:2.30.29.30PH-like_dom_sfGENE3D
PTHR46572RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATEDRho-type_GEFsPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

Orthologs

References / Literature

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:16224022 - Counting cytokinesis proteins globally and locally in fission yeast.
Wu JQ et al. Science 2005 Oct 14;310(5746):310-4
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:37039135 - Cdc42 prevents precocious Rho1 activation during cytokinesis in a Pak1-dependent manner.
Onwubiko UN et al. J Cell Sci 2023 Apr 15;136(8)
PMID:39509469 - Rho1 and Rgf1 establish a new actin-dependent signal to determine growth poles in yeast independently of microtubules and the Tea1-Tea4 complex.
Garcia P et al. PLoS Biol 2024 Nov 07;22(11):e3002491
PMID:16291723 - Cell wall remodeling at the fission yeast cell division site requires the Rho-GEF Rgf3p.
Morrell-Falvey JL et al. J Cell Sci 2005 Dec 01;118(Pt 23):5563-73
PMID:14637153 - Role of guanine nucleotide exchange factors for Rho family GTPases in the regulation of cell morphology and actin cytoskeleton in fission yeast.
Iwaki N et al. Biochem Biophys Res Commun 2003 Dec 12;312(2):414-20
PMID:23394829 - Identification of SIN pathway targets reveals mechanisms of crosstalk between NDR kinase pathways.
Gupta S et al. Curr Biol 2013 Feb 18;23(4):333-8
PMID:28334931 - Rgf1p (Rho1p GEF) is required for double-strand break repair in fission yeast.
Manjón E et al. Nucleic Acids Res 2017 May 19;45(9):5269-5284
PMID:39540318 - Pck2 association with the plasma membrane and efficient response of the cell integrity pathway require regulation of PI4P homeostasis by exomer.
Moscoso-Romero E et al. Open Biol 2024 Nov;14(11):240101
PMID:29689193 - Mechanosensation Dynamically Coordinates Polar Growth and Cell Wall Assembly to Promote Cell Survival.
Davì V et al. Dev Cell 2018 Apr 23;45(2):170-182.e7
PMID:24336750 - Feedback regulation of SIN by Etd1 and Rho1 in fission yeast.
Alcaide-Gavilán M et al. Genetics 2014 Feb;196(2):455-70
PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.
Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787
PMID:19037094 - The Rho1p exchange factor Rgf1p signals upstream from the Pmk1 mitogen-activated protein kinase pathway in fission yeast.
Garcia P et al. Mol Biol Cell 2009 Jan;20(2):721-31
PMID:20739711 - Cooperation between the septins and the actomyosin ring and role of a cell-integrity pathway during cell division in fission yeast.
Wu JQ et al. Genetics 2010 Nov;186(3):897-915
PMID:23738021 - A genome-wide screening of potential target genes to enhance the antifungal activity of micafungin in Schizosaccharomyces pombe.
Zhou X et al. PLoS One 2013;8(5):e65904
PMID:16324155 - Rho1-GEFs Rgf1 and Rgf2 are involved in formation of cell wall and septum, while Rgf3 is involved in cytokinesis in fission yeast.
Mutoh T et al. Genes Cells 2005 Dec;10(12):1189-202
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:29975157 - Analysis of the contribution of phosphoinositides to medial septation in fission yeast highlights the importance of PI(4,5)P 2 for medial contractile ring anchoring.
Snider CE et al. Mol Biol Cell 2018 Sep 01;29(18):2148-2155
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16421249 - Rgf1p is a specific Rho1-GEF that coordinates cell polarization with cell wall biogenesis in fission yeast.
García P et al. Mol Biol Cell 2006 Apr;17(4):1620-31
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:28784611 - Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis.
Snider CE et al. J Cell Biol 2017 Oct 02;216(10):3041-3050
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:19189958 - Fission yeast rgf2p is a rho1p guanine nucleotide exchange factor required for spore wall maturation and for the maintenance of cell integrity in the absence of rgf1p.
García P et al. Genetics 2009 Apr;181(4):1321-34
PMID:23093943 - Cytokinesis-based constraints on polarized cell growth in fission yeast.
Bohnert KA et al. PLoS Genet 2012;8(10):e1003004
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:34666001 - Detection of surface forces by the cell-wall mechanosensor Wsc1 in yeast.
Neeli-Venkata R et al. Dev Cell 2021 Oct 25;56(20):2856-2870.e7
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.
Malecki M et al. Genome Biol 2016 Nov 25;17(1):240
PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.
Malecki M et al. Wellcome Open Res 2016;1:12
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23907979 - The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway.
Cruz S et al. Microbiologyopen 2013 Oct;2(5):778-94
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:19805578 - Genome-wide mapping of myosin protein-RNA networks suggests the existence of specialized protein production sites.
Mata J FASEB J 2010 Feb;24(2):479-84
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:24478458 - The checkpoint-dependent nuclear accumulation of Rho1p exchange factor Rgf1p is important for tolerance to chronic replication stress.
Muñoz S et al. Mol Biol Cell 2014 Apr;25(7):1137-50
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)