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protein coding gene - asf1 (SPCC663.05c) - histone H3-H4 chaperone Asf1

Gene summary

Standard name
asf1
Systematic ID
SPCC663.05c
Product
histone H3-H4 chaperone Asf1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
cia1
UniProt ID
O74515
ORFeome ID
10/10A01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1642902..1644584 reverse strand

Annotation

GO biological process

GO:0006325 - chromatin organization

References:

GO:0006335 - DNA replication-dependent chromatin assembly

References:

GO:0045815 - transcription initiation-coupled chromatin remodeling

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0000510 - H3-H4 histone complex chaperone activity

References:

GO:0042393 - histone binding

References:

GO:0140713 - histone chaperone activity

References:

GO:0005515 - protein binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0004240 - decreased histone H3-K56 acetylation during vegetative growth

References:

Genotypes:

FYPO:0005516 - decreased nucleosome occupancy in euchromatin

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0007339 - increased cen-dg RNA level

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

References:

Genotypes:

FYPO:0002891 - normal chromatin organization during vegetative growth

References:

Genotypes:

FYPO:0004742 - normal chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0002336 - normal chromatin silencing at silent mating-type cassette

References:

Genotypes:

FYPO:0002892 - normal heterochromatin organization during vegetative growth

References:

Genotypes:

FYPO:0005336 - normal level of histone H3 in cell

References:

Genotypes:

FYPO:0008392 - normal level of histone H4 in cell

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

Protein features

PBO:0111951 - Hir protein binding region

References:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0006620 - abnormal chromatin organization during recovery from glucose starvation

References:

Genotypes:

FYPO:0001352 - abnormal chromatin organization during vegetative growth

References:

Genotypes:

FYPO:0002346 - abnormal chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0003247 - abolished histone H3 binding

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0006993 - decreased chromatin silencing at centromere otr1R

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0002827 - decreased chromatin silencing at silent mating-type cassette

References:

Genotypes:

FYPO:0006920 - decreased DNA recombination at mitotic DNA replication fork barriers

References:

Genotypes:

FYPO:0003096 - decreased histone H3-K9 methylation at centromere outer repeat during vegetative growth

References:

Genotypes:

FYPO:0003249 - decreased level of histone H3 at dh repeat

References:

Genotypes:

FYPO:0004578 - decreased level of histone H3 in cell

References:

Genotypes:

FYPO:0007324 - decreased level of histone H4 in cell

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0005288 - decreased negative regulation of transcription by glucose

References:

Genotypes:

FYPO:0008151 - decreased nucleosome occupancy at centromeric heterochromatin

References:

Genotypes:

FYPO:0005516 - decreased nucleosome occupancy in euchromatin

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0003557 - increased antisense RNA level

References:

Genotypes:

FYPO:0007339 - increased cen-dg RNA level

References:

Genotypes:

FYPO:0004347 - increased histone H3-K9 acetylation at protein coding gene during vegetative growth

References:

Genotypes:

FYPO:0003248 - increased level of histone H3 at centromere inner repeat

References:

Genotypes:

FYPO:0000455 - increased number of double-strand break sites during vegetative growth

References:

Genotypes:

FYPO:0000972 - increased number of Rad52 foci during vegetative growth

References:

Genotypes:

FYPO:0000847 - increased protein degradation during vegetative growth

References:

Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0008150 - increased rDNA intergenic repeat RNA level

References:

Genotypes:

FYPO:0005917 - increased subtelomeric heterochromatin RNA level

References:

Genotypes:

FYPO:0002411 - inviable curved elongated vegetative cell

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0001761 - normal mitotic G1/S phase transition

References:

Genotypes:

FYPO:0003246 - normal mitotic S phase progression

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0000838 - normal protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0001317 - normal RNA level during vegetative growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000783 - protein mislocalized to cytoplasm during vegetative growth

References:

Genotypes:

FYPO:0002578 - resistance to hydroxyurea

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0000265 - sensitive to DNA damage

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF04729ASF1_hist_chapASF1-likePfam
G3DSA:2.60.40.1490:FF:000001CATH-FunFam
G3DSA:2.60.40.1490ASF1-like_sfCATH-Gene3D
SSF101546ASF1-like_sfSUPERFAMILY
PTHR12040ASF1-likePANTHER
PIRSF037759Histone_Asf1Hist_deposition_Asf1PIRSF
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMobiDB-Negative-Polyelectrolyte
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:17690098 - Acetylation of lysine 56 of histone H3 catalyzed by RTT109 and regulated by ASF1 is required for replisome integrity.
Han J et al. J Biol Chem 2007 Sep 28;282(39):28587-28596
PMID:21559379 - The HIRA complex subunit Hip3 plays important roles in the silencing of meiosis-specific genes in Schizosaccharomyces pombe.
Mizuki F et al. PLoS One 2011 Apr 29;6(4):e19442
PMID:11856374 - Polyanionic stretch-deleted histone chaperone cia1/Asf1p is functional both in vivo and in vitro.
Umehara T et al. Genes Cells 2002 Jan;7(1):59-73
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:22683458 - Interacting factors and cellular localization of SR protein-specific kinase Dsk1.
Tang Z et al. Exp Cell Res 2012 Oct 01;318(16):2071-84
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:33468217 - Rbm10 facilitates heterochromatin assembly via the Clr6 HDAC complex.
Weigt M et al. Epigenetics Chromatin 2021 Jan 19;14(1):8
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:14680630 - Structure and function of the conserved core of histone deposition protein Asf1.
Daganzo SM et al. Curr Biol 2003 Dec 16;13(24):2148-58
PMID:18334479 - Crystal structures of fission yeast histone chaperone Asf1 complexed with the Hip1 B-domain or the Cac2 C terminus.
Malay AD et al. J Biol Chem 2008 May 16;283(20):14022-31
PMID:31584934 - Histone deposition promotes recombination-dependent replication at arrested forks.
Hardy J et al. PLoS Genet 2019 Oct;15(10):e1008441
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:29967244 - Histone Chaperone Asf1 Is Required for the Establishment of Repressive Chromatin in Schizosaccharomyces pombe fbp1 Gene Repression.
Umeda M et al. Mol Cell Biol 2018 Sep 15;38(18)
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:21324894 - Nuclear protein quality is regulated by the ubiquitin-proteasome system through the activity of Ubc4 and San1 in fission yeast.
Matsuo Y et al. J Biol Chem 2011 Apr 15;286(15):13775-90
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:39878217 - The ortholog of human DNAJC9 promotes histone H3-H4 degradation and is counteracted by Asf1 in fission yeast.
Ding Y et al. Nucleic Acids Res 2025 Jan 24;53(3)
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:22291963 - Histone chaperone Asf1 plays an essential role in maintaining genomic stability in fission yeast.
Tanae K et al. PLoS One 2012;7(1):e30472
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16511210 - Purification, crystallization and preliminary X-ray diffraction analysis of the histone chaperone cia1 from fission yeast.
Umehara T et al. Acta Crystallogr Sect F Struct Biol Cryst Commun 2005 Nov 01;61(Pt 11):971-3
PMID:23089178 - Sim3 shares some common roles with the histone chaperone Asf1 in fission yeast.
Tanae K et al. FEBS Lett 2012 Nov 30;586(23):4190-6
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:21211723 - Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci.
Yamane K et al. Mol Cell 2011 Jan 07;41(1):56-66
GO_REF:0000051 - S. pombe keyword mapping