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protein coding gene - cid12 (SPCC663.12) - poly(A) polymerase Cid12

Gene summary

Standard name
cid12
Systematic ID
SPCC663.12
Product
poly(A) polymerase Cid12
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
csp8, csp10
UniProt ID
O74518
ORFeome ID
15/15A07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1655646..1660080 forward strand

Annotation

Comment

PBO:0015249 - RDRC and RITS complexes associate together, and with non-coding centromeric RNA in a Dcr1 (hence siRNA) and Clr4 dependent manner

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GO biological process

GO:1990431 - priRNA 3'-end processing

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GO:0031048 - regulatory ncRNA-mediated heterochromatin formation

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GO:0030466 - silent mating-type cassette heterochromatin formation

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GO:0140727 - siRNA-mediated pericentric heterochromatin formation

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GO cellular component

GO:0005737 - cytoplasm

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GO:0005829 - cytosol

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GO:0031934 - mating-type region heterochromatin

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GO:0031380 - nuclear RNA-directed RNA polymerase complex

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GO:0005634 - nucleus

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GO:0005721 - pericentric heterochromatin

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GO:0031379 - RNA-directed RNA polymerase complex

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GO molecular function

GO:1990817 - poly(A) RNA polymerase activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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Multi-locus phenotype

FYPO:0000173 - abnormal mitotic cell cycle DNA replication checkpoint

References:

Genotypes:

FYPO:0005045 - abnormal mitotic sister chromatid segregation with lagging chromosomes and decreased rate of mitotic spindle elongation

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Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0004201 - decreased centromeric outer repeat transcript-derived siRNA level

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Genotypes:

FYPO:0000888 - decreased histone H3-K9 dimethylation at centromere outer repeat during vegetative growth

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Genotypes:

FYPO:0006073 - decreased small RNA degradation

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001387 - loss of viability at high temperature

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Genotypes:

FYPO:0000283 - mitotic chromosome fragmentation upon segregation

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Genotypes:

FYPO:0002837 - normal centromeric outer repeat transcript-derived siRNA level

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003625 - abnormal microtubule cytoskeleton morphology during mitotic interphase

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Genotypes:

FYPO:0000326 - abnormal mitotic sister chromatid biorientation

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Genotypes:

FYPO:0005046 - abnormal mitotic sister chromatid segregation with lagging chromosomes, complete sister chromatid separation, and decreased rate of mitotic spindle elongation

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Genotypes:

FYPO:0003263 - abnormal sporulation resulting in formation of ascus with more than four spores

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Genotypes:

FYPO:0008235 - abolished nuclear RNA-directed RNA polymerase complex assembly

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Genotypes:

FYPO:0008011 - abolished siRNA loading onto RITS complex

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Genotypes:

FYPO:0001270 - complete but unequal mitotic sister chromatid segregation

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Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

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Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

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Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

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Genotypes:

FYPO:0004201 - decreased centromeric outer repeat transcript-derived siRNA level

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Genotypes:

FYPO:0002834 - decreased chromatin silencing at centromere

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Genotypes:

FYPO:0003411 - decreased chromatin silencing at centromere inner repeat

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Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0000460 - decreased mitotic centromeric sister chromatid cohesion

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Genotypes:

FYPO:0000450 - decreased protein localization to centromere during vegetative growth

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Genotypes:

FYPO:0002822 - decreased protein localization to mitotic spindle pole body during mitosis

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Genotypes:

FYPO:0002386 - decreased protein localization to pericentric heterochromatin at centromere outer repeat region

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0001271 - incomplete, unequal mitotic sister chromatid segregation

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Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

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Genotypes:

FYPO:0009077 - increased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009101 - increased cell population growth on glycerol and galactose carbon source

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Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

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Genotypes:

FYPO:0000220 - increased centromeric outer repeat transcript level

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Genotypes:

FYPO:0000618 - increased duration of mitotic anaphase

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Genotypes:

FYPO:0003351 - increased duration of protein localization to kinetochore during vegetative growth

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Genotypes:

FYPO:0001840 - increased minichromosome loss during vegetative growth

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Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

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Genotypes:

FYPO:0005978 - increased spliced cen-dg RNA level

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Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0000228 - lagging mitotic chromosomes

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

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Genotypes:

FYPO:0001513 - normal mitotic sister chromatid segregation

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Genotypes:

FYPO:0002928 - normal poly(A) tail length

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Genotypes:

FYPO:0002574 - normal protein localization to centromere during vegetative growth

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Genotypes:

FYPO:0001317 - normal RNA level during vegetative growth

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0009031 - resistance to bleomycin

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Genotypes:

FYPO:0009032 - resistance to bortezomib

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0001884 - resistance to Calcofluor White

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009034 - resistance to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000077 - resistance to rapamycin

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Genotypes:

FYPO:0005266 - resistance to sodium dodecyl sulfate

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Genotypes:

FYPO:0005193 - resistance to torin1

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Genotypes:

FYPO:0007808 - resistance to valproic acid

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0007921 - sensitive to benzamidine

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Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

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Genotypes:

FYPO:0004586 - sensitive to caffeine and hydroxyurea

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007938 - sensitive to tea tree oil

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0006076 - siRNA absent from cell

References:

Genotypes:

FYPO:0003182 - sister chromatid nondisjunction at meiosis II

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Genotypes:

FYPO:0005634 - sister kinetochore dissociation in meiotic metaphase I with equational sister chromatid segregation in meiosis I

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Genotypes:

FYPO:0007474 - variable cell size at division

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Genotypes:

FYPO:0007660 - variable cell size at division with subsequent division at abnormal size

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0001510 - viable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0000055 - no apparent S. cerevisiae ortholog

Protein features

IDNameInterPro nameDB name
PF03828PAP_assocPAP_assocPfam
PF22600MTPAP-like_centralMTPAP-like_centralPfam
cd05402NT_PAP_TUTaseCDD
G3DSA:1.10.1410.10CATH-Gene3D
G3DSA:3.30.460.10NT_sfCATH-Gene3D
SSF81301NT_sfSUPERFAMILY
SSF81631SUPERFAMILY
PTHR23092Trf4-likePANTHER

Orthologs

References / Literature

PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:10757807 - Cid1, a fission yeast protein required for S-M checkpoint control when DNA polymerase delta or epsilon is inactivated.
Wang SW et al. Mol Cell Biol 2000 May;20(9):3234-44
PMID:23572080 - Distinct roles for Sir2 and RNAi in centromeric heterochromatin nucleation, spreading and maintenance.
Buscaino A et al. EMBO J 2013 May 02;32(9):1250-64
PMID:33419777 - Identification of mutants with increased variation in cell size at onset of mitosis in fission yeast.
Scotchman E et al. J Cell Sci 2021 Feb 11;134(3)
PMID:19164572 - Msc1 links dynamic Swi6/HP1 binding to cell fate determination.
Lawrence RJ et al. Proc Natl Acad Sci U S A 2009 Jan 27;106(4):1163-8
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:25274039 - A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeast.
Bayne EH et al. Genome Biol 2014;15(10):481
PMID:20705239 - The methyltransferase activity of Clr4Suv39h triggers RNAi independently of histone H3K9 methylation.
Gerace EL et al. Mol Cell 2010 Aug 13;39(3):360-72
PMID:25373780 - A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.
Graml V et al. Dev Cell 2014 Oct 27;31(2):227-239
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:40193710 - An enzymatic-independent function of palmitoyl hydrolase in cohesin loading onto chromosome.
Wang YT et al. Nucleic Acids Res 2025 Mar 20;53(6)
PMID:15607976 - Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs.
Motamedi MR et al. Cell 2004 Dec 17;119(6):789-802
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16478992 - Requirement of fission yeast Cid14 in polyadenylation of rRNAs.
Win TZ et al. Mol Cell Biol 2006 Mar;26(5):1710-21
PMID:16738311 - Fission yeast Cid12 has dual functions in chromosome segregation and checkpoint control.
Win TZ et al. Mol Cell Biol 2006 Jun;26(12):4435-47
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:21289066 - Elimination of a specific histone H3K14 acetyltransferase complex bypasses the RNAi pathway to regulate pericentric heterochromatin functions.
Reddy BD et al. Genes Dev 2011 Feb 01;25(3):214-9
PMID:22139915 - The telomeric transcriptome of Schizosaccharomyces pombe.
Bah A et al. Nucleic Acids Res 2012 Apr;40(7):2995-3005
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:22474355 - Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly.
Hayashi A et al. Proc Natl Acad Sci U S A 2012 Apr 17;109(16):6159-64
PMID:11069763 - Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast.
Pidoux AL et al. J Cell Sci 2000 Dec;113 Pt 23:4177-91
PMID:27538348 - Set3 contributes to heterochromatin integrity by promoting transcription of subunits of Clr4-Rik1-Cul4 histone methyltransferase complex in fission yeast.
Yu Y et al. Sci Rep 2016 Aug 19;6:31752
PMID:28228545 - Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.
Upadhyay U et al. Mol Biol Cell 2017 Apr 15;28(8):1132-1146
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:18948543 - Splicing factors facilitate RNAi-directed silencing in fission yeast.
Bayne EH et al. Science 2008 Oct 24;322(5901):602-6
PMID:20178743 - Dicer-independent primal RNAs trigger RNAi and heterochromatin formation.
Halic M et al. Cell 2010 Feb 19;140(4):504-16
PMID:28231281 - The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin.
Mutazono M et al. PLoS Genet 2017 Feb;13(2):e1006606
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:28541282 - Tailing and degradation of Argonaute-bound small RNAs protect the genome from uncontrolled RNAi.
Pisacane P et al. Nat Commun 2017 May 25;8:15332
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:22733737 - Ers1 links HP1 to RNAi.
Rougemaille M et al. Proc Natl Acad Sci U S A 2012 Jul 10;109(28):11258-63
PMID:27053105 - Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast.
Burr R et al. J Biol Chem 2016 Jun 03;291(23):12171-83
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.
Malecki M et al. Genome Biol 2016 Nov 25;17(1):240
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:37445861 - Resistance to Chemotherapeutic 5-Fluorouracil Conferred by Modulation of Heterochromatic Integrity through Ino80 Function in Fission Yeast.
Lim KK et al. Int J Mol Sci 2023 Jun 26;24(13)
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7