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protein coding gene - gma12 (SPCC736.04c) - Golgi alpha-1,2-galactosyltransferase Gma12

Gene summary

Standard name
gma12
Systematic ID
SPCC736.04c
Product
Golgi alpha-1,2-galactosyltransferase Gma12
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q09174
ORFeome ID
17/17G02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 315827..317852 reverse strand

Annotation

PBO:0000169 - 2.4.1.-

GO biological process

GO:0006487 - protein N-linked glycosylation

References:

GO:0006493 - protein O-linked glycosylation

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GO cellular component

GO:0005794 - Golgi apparatus

References:

GO:0000139 - Golgi membrane

References:

GO:0140497 - mannan polymerase II complex

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GO molecular function

GO:0031278 - alpha-1,2-galactosyltransferase activity

References:

Multi-locus phenotype

FYPO:0006277 - abnormal N-linked glycoprotein glycan structure

References:

Genotypes:

FYPO:0006278 - abnormal O-linked glycoprotein glycan structure

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Genotypes:

FYPO:0003354 - decreased galactose level in glycoprotein glycan

References:

Genotypes:

FYPO:0003447 - decreased level of glycosylated protein in cell

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0003291 - galactose absent from glycoprotein glycan

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0001120 - pear-shaped vegetative cell

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Genotypes:

FYPO:0000106 - sensitive to hygromycin B

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Genotypes:

FYPO:0000021 - spheroid vegetative cell

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Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000018 - protein level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000164 - abnormal cell separation after cytokinesis

References:

Genotypes:

FYPO:0003625 - abnormal microtubule cytoskeleton morphology during mitotic interphase

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Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

References:

Genotypes:

FYPO:0006807 - decreased galactose level in cell surface glycoprotein glycan

References:

Genotypes:

FYPO:0003354 - decreased galactose level in glycoprotein glycan

References:

Genotypes:

FYPO:0006806 - decreased galactosyltransferase activity

References:

Genotypes:

FYPO:0003447 - decreased level of glycosylated protein in cell

References:

Genotypes:

FYPO:0009101 - increased cell population growth on glycerol and galactose carbon source

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Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009071 - sensitive to itraconazole

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0001510 - viable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

Protein features

IDNameInterPro nameDB name
PF05637Glyco_transf_34Glyco_trans_34Pfam
G3DSA:3.90.550.10Nucleotide-diphossugar_transCATH-Gene3D
PTHR31306Glyco_trans_34PANTHER
Signal PeptideSignal-Peptide-DeepTMHMM

Orthologs

References / Literature

PMID:9839953 - Differences in in vivo acceptor specificity of two galactosyltransferases, the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe.
Yoko-o T et al. Eur J Biochem 1998 Nov 01;257(3):630-7
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25373780 - A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.
Graml V et al. Dev Cell 2014 Oct 27;31(2):227-239
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:21098516 - Structural analysis of α1,3-linked galactose-containing oligosaccharides in Schizosaccharomyces pombe mutants harboring single and multiple α-galactosyltransferase genes disruptions.
Ohashi T et al. Glycobiology 2011 Mar;21(3):340-51
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:33909078 - Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
Fukunaga T et al. Glycobiology 2021 Sep 09;31(8):1037-1045
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:41259369 - Hydroxyurea induces an oxidative stress response that triggers ER expansion and cytoplasmic protein aggregation.
Sánchez-Molina A et al. PLoS Biol 2025 Nov 19;23(11):e3003493
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:20531409 - Global coordination of transcriptional control and mRNA decay during cellular differentiation.
Amorim MJ et al. Mol Syst Biol 2010 Jun 08;6:380
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:22988247 - Identification of novel α1,3-galactosyltransferase and elimination of α-galactose-containing glycans by disruption of multiple α-galactosyltransferase genes in Schizosaccharomyces pombe.
Ohashi T et al. J Biol Chem 2012 Nov 09;287(46):38866-75
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:11284010 - Hut1 proteins identified in Saccharomyces cerevisiae and Schizosaccharomyces pombe are functional homologues involved in the protein-folding process at the endoplasmic reticulum.
Nakanishi H et al. Yeast 2001 Apr;18(6):543-54
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:7522655 - Localization of an alpha 1,2 galactosyltransferase activity to the Golgi apparatus of Schizosaccharomyces pombe.
Chappell TG et al. Mol Biol Cell 1994 May;5(5):519-28
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:22907753 - Posttranscriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in Schizosaccharomyces pombe.
Wells ML et al. Mol Cell Biol 2012 Oct;32(20):4206-14
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623