protein coding gene - dar2 (SPCC736.06) - mitochondrial aspartate-tRNA ligase Dar2

Gene summary

Standard name: dar2
Systematic ID: SPCC736.06
Product: mitochondrial aspartate-tRNA ligase Dar2
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: O94242
ORFeome ID: 46/46A03
Characterisation status: biological role inferred
Feature type: mRNA gene
Genomic location: chromosome III: 322498..326767 forward strand

Annotation

Disease association

MONDO:0980969 - Charcot-Marie-Tooth disease, axonal, type 2LL

References:

PB_REF:0000006

MONDO:0012622 - leukoencephalopathy with brain stem and spinal cord involvement-high lactate syndrome

References:

PB_REF:0000006

GO biological process

GO:0006422 - aspartyl-tRNA aminoacylation

References:

GO_REF:0000033

GO cellular component

GO:0005759 - mitochondrial matrix

References:

GO_REF:0000111

GO:0005739 - mitochondrion

References:

PMID:16823372

GO molecular function

GO:0004815 - aspartate-tRNA ligase activity

References:

GO_REF:0000033

GO:0005524 - ATP binding

References:

GO_REF:0000002

Modification

MOD:00047 - O-phospho-L-threonine

References:

PMID:30726745

Protein features

PBO:0111871 - OB fold

Protein sequence feature

SO:0001808 - mitochondrial_targeting_signal

References:

PMID:36408920

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

PMID:33313903

Quantitative gene expression

PBO:0006310 - protein level

References:

PMID:23101633

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

PMID:27887640

Genotypes:

dar2delta

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

dar2delta

FYPO:0002111 - inviable tapered vegetative cell

References:

PMID:23697806

Genotypes:

dar2delta

FYPO:0004325 - sensitive to 5-fluorouracil

References:

PMID:25483073

Genotypes:

dar2delta

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

dar2delta

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

PMID:23697806

Genotypes:

dar2delta

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF00152	tRNA-synt_2	Aa-tRNA-synt_II	PFAM
PF01336	tRNA_anti-codon	NA-bd_OB_tRNA	PFAM
cd04321	ScAspRS_mt_like_N		CDD
cd00777	AspRS_core	AspRS_core	CDD
PS50862	AA_TRNA_LIGASE_II	aa-tRNA-synth_II	PROSITE_PROFILES
PR01042	TRNASYNTHASP	Asp/Asn-tRNA-synth_IIb	PRINTS
SSF55681	Class II aaRS and biotin synthetases	aa-tRNA-synth_II/BPL/LPL	SUPERFAMILY
SSF50249	Nucleic acid-binding proteins	NA-bd_OB-fold	SUPERFAMILY
G3DSA:2.40.50.140		NA-bd_OB-fold	GENE3D
G3DSA:3.30.930.10	Bira Bifunctional Protein; Domain 2	aa-tRNA-synth_II/BPL/LPL	GENE3D
G3DSA:3.30.1360.30		GAD-like_sf	GENE3D
PTHR22594	ASPARTYL/LYSYL-TRNA SYNTHETASE		PANTHER
MF_00044	Asp_tRNA_synth_type1	Asp-tRNA-ligase_1	HAMAP
TIGR00459	aspS_bact	Asp-tRNA-ligase_1	NCBIFAM
NF001750	PRK00476.1	Asp-tRNA-ligase_1	NCBIFAM

Orthologs

DARS2 (HGNC:25538) Homo sapiens
dar2 (SJAG_04094) Schizosaccharomyces japonicus
MSD1 (YPL104W) Saccharomyces cerevisiae

References / Literature

PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.: Malecki M et al. Genome Biol 2016 Nov 25;17(1):240
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.: Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000002 - Comments
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.: Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:26098123 - Promoter nucleosome dynamics regulated by signalling through the CTD code.: Materne P et al. Elife 2015 Jun 22;4:e09008
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.: Malecki M et al. Wellcome Open Res 2016;1:12
PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.: Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.: UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623