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protein coding gene - hmt1 (SPCC737.09c) - vacuolar phytochelatin and glutathione S-conjugate ABC family transmembrane transporter Hmt1

Gene summary

Standard name
hmt1
Systematic ID
SPCC737.09c
Product
vacuolar phytochelatin and glutathione S-conjugate ABC family transmembrane transporter Hmt1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPCC74.08c
UniProt ID
Q02592
ORFeome ID
47/47A02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1914756..1917671 reverse strand

Annotation

Complementation

PBO:0010259 - functionally complemented by D. melanogaster HMT1(CG4225)

References:

Disease association

MONDO:0014169 - dyschromatosis universalis hereditaria 3

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MONDO:0012204 - familial pseudohyperkalemia

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MONDO:0013783 - microphthalmia, isolated, with coloboma 7

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GO biological process

GO:0036249 - cadmium ion import into vacuole

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GO:0098849 - cellular detoxification of cadmium ion

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GO:0071996 - glutathione transmembrane import into vacuole

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GO:0036246 - phytochelatin 2 import into vacuole

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GO:0071995 - phytochelatin import into vacuole

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GO cellular component

GO:0000324 - fungal-type vacuole

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GO:0000329 - fungal-type vacuole membrane

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GO molecular function

GO:0044604 - ABC-type phytochelatin transporter activity

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GO:0005524 - ATP binding

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GO:0016887 - ATP hydrolysis activity

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GO:0042626 - ATPase-coupled transmembrane transporter activity

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Modification

MOD:00006 - N-glycosylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0004911 - abolished glutathione import into vacuole

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Genotypes:

FYPO:0002829 - decreased cellular phytochelatin level

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Genotypes:

FYPO:0000741 - increased cellular pigment accumulation

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Genotypes:

FYPO:0004247 - normal vacuolar morphology during vegetative growth

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Genotypes:

FYPO:0004912 - phytochelatin absent from vacuole

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Protein features

PBO:0111874 - ABC transporter family

Protein sequence feature

SO:0000418 - signal_peptide

References:

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000011 - RNA level increased

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PomGeneEx:0000013 - RNA level unchanged

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0004370 - decreased cadmium ion import into vacuole

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0002829 - decreased cellular phytochelatin level

References:

Genotypes:

FYPO:0004369 - decreased vacuolar phytochelatin level

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Genotypes:

FYPO:0004368 - normal growth on arsenic

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Genotypes:

FYPO:0001023 - normal growth on cisplatin

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Genotypes:

FYPO:0003849 - normal growth on fluconazole

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Genotypes:

FYPO:0003852 - normal growth on FR901464

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Genotypes:

FYPO:0003508 - normal growth on mercury

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Genotypes:

FYPO:0000979 - normal growth on miconazole

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Genotypes:

FYPO:0003851 - normal growth on MTT

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Genotypes:

FYPO:0003850 - normal growth on oligomycin

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000327 - resistance to trichostatin A

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Genotypes:

FYPO:0000093 - sensitive to arsenic

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0002237 - sensitive to cerulenin

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Genotypes:

FYPO:0000102 - sensitive to cisplatin

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Genotypes:

FYPO:0002640 - sensitive to clotrimazole

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00664ABC_membraneABC1_TM_domPFAM
PF00005ABC_tranABC_transporter-like_ATP-bdPFAM
cd03253ABCC_ATM1_transporterCDD
cd18583ABC_6TM_HMT1CDD
PS00211ABC_TRANSPORTER_1ABC_transporter-like_CSPROSITE_PATTERNS
PS50929ABC_TM1FABC1_TM_domPROSITE_PROFILES
PS50893ABC_TRANSPORTER_2ABC_transporter-like_ATP-bdPROSITE_PROFILES
SM00382AAA_5AAA+_ATPaseSMART
G3DSA:1.20.1560.10:FF:000050FUNFAM
G3DSA:3.40.50.300:FF:000186FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
SSF90123ABC transporter transmembrane regionABC1_TM_sfSUPERFAMILY
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
G3DSA:1.20.1560.10ABC transporter type 1, transmembrane domainABC1_TM_sfGENE3D
PTHR24221ATP-BINDING CASSETTE SUB-FAMILY BType_1_exporterPANTHER
CoilCoilCOILS

Orthologs

References / Literature

PMID:20937798 - Tonoplast-localized Abc2 transporter mediates phytochelatin accumulation in vacuoles and confers cadmium tolerance.
Mendoza-Cózatl DG et al. J Biol Chem 2010 Dec 24;285(52):40416-26
PMID:9017391 - apd1+, a gene required for red pigment formation in ade6 mutants of Schizosaccharomyces pombe, encodes an enzyme required for glutathione biosynthesis: a role for glutathione and a glutathione-conjugate pump.
Chaudhuri B et al. Genetics 1997 Jan;145(1):75-83
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:9016341 - Role of determinants of cadmium sensitivity in the tolerance of Schizosaccharomyces pombe to cisplatin.
Perego P et al. Mol Pharmacol 1997 Jan;51(1):12-8
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16849797 - A survey of all 11 ABC transporters in fission yeast: two novel ABC transporters are required for red pigment accumulation in a Schizosaccharomyces pombe adenine biosynthetic mutant.
Iwaki T et al. Microbiology (Reading) 2006 Aug;152(Pt 8):2309-2321
PMID:25795664 - Genetic Interaction Landscape Reveals Critical Requirements for Schizosaccharomyces pombe Brc1 in DNA Damage Response Mutants.
Sánchez A et al. G3 (Bethesda) 2015 Mar 19;5(5):953-62
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.
Malecki M et al. Wellcome Open Res 2016;1:12
PMID:27558664 - Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast.
Guo L et al. G3 (Bethesda) 2016 Oct 13;6(10):3317-3333
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:1396551 - Heavy metal tolerance in the fission yeast requires an ATP-binding cassette-type vacuolar membrane transporter.
Ortiz DF et al. EMBO J 1992 Oct;11(10):3491-9
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:19915076 - abc3+ encodes an iron-regulated vacuolar ABC-type transporter in Schizosaccharomyces pombe.
Pouliot B et al. Eukaryot Cell 2010 Jan;9(1):59-73
PMID:20221439 - Detoxification of multiple heavy metals by a half-molecule ABC transporter, HMT-1, and coelomocytes of Caenorhabditis elegans.
Schwartz MS et al. PLoS One 2010 Mar 05;5(3):e9564
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:7876244 - Transport of metal-binding peptides by HMT1, a fission yeast ABC-type vacuolar membrane protein.
Ortiz DF et al. J Biol Chem 1995 Mar 03;270(9):4721-8
PMID:22840777 - Analyzing fission yeast multidrug resistance mechanisms to develop a genetically tractable model system for chemical biology.
Kawashima SA et al. Chem Biol 2012 Jul 27;19(7):893-901
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:12868054 - Characterization of vps33+, a gene required for vacuolar biogenesis and protein sorting in Schizosaccharomyces pombe.
Iwaki T et al. Yeast 2003 Jul 30;20(10):845-55
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:19001374 - Drosophila ABC transporter, DmHMT-1, confers tolerance to cadmium. DmHMT-1 and its yeast homolog, SpHMT-1, are not essential for vacuolar phytochelatin sequestration.
Sooksa-Nguan T et al. J Biol Chem 2009 Jan 02;284(1):354-362
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.
Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
GO_REF:0000002 - Comments
PMID:19054771 - A common highly conserved cadmium detoxification mechanism from bacteria to humans: heavy metal tolerance conferred by the ATP-binding cassette (ABC) transporter SpHMT1 requires glutathione but not metal-chelating phytochelatin peptides.
Prévéral S et al. J Biol Chem 2009 Feb 20;284(8):4936-43
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6