protein coding gene - ssp2 (SPCC74.03c) - serine/threonine protein kinase AMPK catalytic (alpha) subunit Ssp2

Gene summary

Standard name

ssp2

Systematic ID

SPCC74.03c

Product

serine/threonine protein kinase AMPK catalytic (alpha) subunit Ssp2

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

ucp9

UniProt ID

O74536

ORFeome ID

27/27F02

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome III: 1929190..1931974 reverse strand

Annotation

Protein features

Orthologs

• PRKAA1 (HGNC:9376) Homo sapiens
• PRKAA2 (HGNC:9377) Homo sapiens
• ssp2 (SJAG_03501) Schizosaccharomyces japonicus
• SNF1 (YDR477W) Saccharomyces cerevisiae

References / Literature

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:24928510 - Glucose activates TORC2-Gad8 protein via positive regulation of the cAMP/cAMP-dependent protein kinase A (PKA) pathway and negative regulation of the Pmk1 protein-mitogen-activated protein kinase pathway.

Cohen A et al. J Biol Chem 2014 Aug 01;289(31):21727-37

PMID:32441227 - Glucose limitation and pka1 deletion rescue aberrant mitotic spindle formation induced by Mal3 overexpression in Schizosaccharomyces pombe .

Tanabe T et al. Biosci Biotechnol Biochem 2020 Aug;84(8):1667-1680

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:30814334 - Import of extracellular ATP in yeast and man modulates AMPK and TORC1 signalling.

Forte GM et al. J Cell Sci 2019 Apr 03;132(7)

PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.

Li J et al. Nat Commun 2019 Mar 01;10(1):1002

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:32053662 - AMPKα Subunit Ssp2 and Glycogen Synthase Kinases Gsk3/Gsk31 are involved in regulation of sterol regulatory element-binding protein (SREBP) activity in fission yeast.

Miao H et al. PLoS One 2020;15(2):e0228845

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:19371376 - Schizosaccharomyces pombe cell division cycle under limited glucose requires Ssp1 kinase, the putative CaMKK, and Sds23, a PP2A-related phosphatase inhibitor.

Hanyu Y et al. Genes Cells 2009 May;14(5):539-54

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

PMID:15821139 - Systematic deletion analysis of fission yeast protein kinases.

Bimbó A et al. Eukaryot Cell 2005 Apr;4(4):799-813

PMID:22375066 - AMPK phosphorylation by Ssp1 is required for proper sexual differentiation in fission yeast.

Valbuena N et al. J Cell Sci 2012 Jun 01;125(Pt 11):2655-64

PMID:19474788 - Structural insight into the autoinhibition mechanism of AMP-activated protein kinase.

Chen L et al. Nature 2009 Jun 25;459(7250):1146-9

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:17289942 - Crystal structures of the adenylate sensor from fission yeast AMP-activated protein kinase.

Townley R et al. Science 2007 Mar 23;315(5819):1726-9

PMID:22140232 - Snf1-like protein kinase Ssp2 regulates glucose derepression in Schizosaccharomyces pombe.

Matsuzawa T et al. Eukaryot Cell 2012 Feb;11(2):159-67

PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.

Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902

PMID:7628434 - A novel protein kinase gene ssp1+ is required for alteration of growth polarity and actin localization in fission yeast.

Matsusaka T et al. EMBO J 1995 Jul 17;14(14):3325-38

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:31562247 - Glucose starvation induces mitochondrial fragmentation depending on the dynamin GTPase Dnm1/Drp1 in fission yeast.

Zheng F et al. J Biol Chem 2019 Nov 22;294(47):17725-17734

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:23370392 - A knockout screen for protein kinases required for the proper meiotic segregation of chromosomes in the fission yeast Schizosaccharomyces pombe.

Kovacikova I et al. Cell Cycle 2013 Feb 15;12(4):618-24

GO_REF:0000002 - Comments

PMID:17937917 - Structural insight into AMPK regulation: ADP comes into play.

Jin X et al. Structure 2007 Oct;15(10):1285-95

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:21680738 - Glucose controls phosphoregulation of hydroxymethylglutaryl coenzyme A reductase through the protein phosphatase 2A-related phosphatase protein, Ppe1, and Insig in fission yeast.

Burg JS et al. J Biol Chem 2011 Aug 05;286(31):27139-46

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Lee J et al. Nat Commun 2017 Feb 20;8:14527

PMID:31030285 - CoQ 10 production in Schizosaccharomyces pombe is increased by reduction of glucose levels or deletion of pka1.

Nishida I et al. Appl Microbiol Biotechnol 2019 Jun;103(12):4899-4915

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.

Rallis C et al. Sci Rep 2017 Mar 10;7:44257

PMID:28515144 - Transient activation of fission yeast AMPK is required for cell proliferation during osmotic stress.

Schutt KL et al. Mol Biol Cell 2017 Jul 01;28(13):1804-1814

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:8886983 - The fission yeast sts5+ gene is required for maintenance of growth polarity and functionally interacts with protein kinase C and an osmosensing MAP-kinase pathway.

Toda T et al. J Cell Sci 1996 Sep;109 ( Pt 9):2331-42

PMID:28600551 - Epe1 contributes to activation of AMPK by promoting phosphorylation of AMPK alpha subunit, Ssp2.

Chen Y et al. Sci Rep 2017 Jun 09;7(1):3208

PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.

Egan ED et al. RNA 2014 Jun;20(6):867-81

PMID:29079657 - TORC1 and TORC2 converge to regulate the SAGA co-activator in response to nutrient availability.

Laboucarié T et al. EMBO Rep 2017 Dec;18(12):2197-2218

PMID:20501954 - Search for kinases related to transition of growth polarity in fission yeast.

Koyano T et al. Biosci Biotechnol Biochem 2010;74(5):1129-33

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.

Larochelle M et al. J Cell Sci 2019 May 31;132(11)

PMID:21445296 - Mis17 is a regulatory module of the Mis6-Mal2-Sim4 centromere complex that is required for the recruitment of CenH3/CENP-A in fission yeast.

Shiroiwa Y et al. PLoS One 2011 Mar 21;6(3):e17761

PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.

Malecki M et al. Wellcome Open Res 2016;1:12

PMID:28223368 - Phosphatases Generate Signal Specificity Downstream of Ssp1 Kinase in Fission Yeast.

Deng L et al. Mol Cell Biol 2017 May 15;37(10)

PMID:25639242 - Nitrogen regulates AMPK to control TORC1 signaling.

Davie E et al. Curr Biol 2015 Feb 16;25(4):445-54

PMID:38780300 - Nitrogen availability is important for preventing catastrophic mitosis in fission yeast.

Zemlianski V et al. J Cell Sci 2024 May 23;

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:27604537 - Genetic Interactions among AMPK Catalytic Subunit Ssp2 and Glycogen Synthase Kinases Gsk3 and Gsk31 in Schizosaccharomyces Pombe.

Qingyun et al. Kobe J Med Sci 2016 Aug 03;62(3):E70-8