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protein coding gene - mak3 (SPCC74.06) - phosphorelay sensor kinase Mak3

Gene summary

Standard name
mak3
Systematic ID
SPCC74.06
Product
phosphorelay sensor kinase Mak3
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
phk2
UniProt ID
O74539
ORFeome ID
34/34C10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1940315..1947736 forward strand

Annotation

Complementation

PBO:0091520 - mak1/mak2/mak3 triple deletion functionally complemented by Candida albicans CHK1

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GO biological process

GO:0000160 - phosphorelay signal transduction system

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GO:1900745 - positive regulation of p38MAPK cascade

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GO cellular component

GO:0005737 - cytoplasm

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GO:0005829 - cytosol

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GO:0009365 - protein histidine kinase complex

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GO:0005634 - nucleus

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GO molecular function

GO:0000155 - phosphorelay sensor kinase activity

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GO:0005515 - protein binding

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Modification

MOD:00042 - L-aspartic 4-phosphoric anhydride

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MOD:00046 - O-phospho-L-serine

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MOD:00890 - phosphorylated L-histidine

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Multi-locus phenotype

FYPO:0006184 - altered level of osmotic stress responsive gene mRNA during vegetative growth

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Genotypes:

FYPO:0002446 - decreased protein phosphorylation during cellular response to hydrogen peroxide

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Genotypes:

FYPO:0002172 - increased level of nitrogen starvation gene mRNA during vegetative growth

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Genotypes:

FYPO:0001043 - increased mating efficiency

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Genotypes:

FYPO:0003031 - mating without nitrogen starvation

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Genotypes:

FYPO:0006107 - mating, meiosis, and sporulation without starvation

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Genotypes:

FYPO:0001091 - normal cellular response to heat shock

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Genotypes:

FYPO:0001485 - normal cellular response to oxidative stress

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Genotypes:

FYPO:0000968 - normal cellular response to stress during vegetative growth

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Genotypes:

FYPO:0001281 - normal protein phosphorylation during cellular response to hydrogen peroxide

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Genotypes:

FYPO:0002052 - normal sporulation frequency

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Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

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Genotypes:

FYPO:0000852 - resistance to salt stress

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0001408 - sensitive to heat shock

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Genotypes:

FYPO:0000081 - sensitive to high osmolarity

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0002865 - sensitive to polypeptone

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Genotypes:

FYPO:0000271 - sensitive to salt stress

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Genotypes:

FYPO:0000112 - sensitive to sorbitol

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Genotypes:

FYPO:0001492 - viable elongated vegetative cell

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Genotypes:

FYPO:0000648 - viable small vegetative cell

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Genotypes:

FYPO:0002106 - viable stubby vegetative cell

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Protein features

PBO:0111893 - PAC domain protein

Qualitative gene expression

PomGeneEx:0000027 - ribosomal density decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0002924 - decreased cell population growth on maltose carbon source

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Genotypes:

FYPO:0002446 - decreased protein phosphorylation during cellular response to hydrogen peroxide

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Genotypes:

FYPO:0001116 - decreased RNA level during cellular response to hydrogen peroxide

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Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

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Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

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Genotypes:

FYPO:0001043 - increased mating efficiency

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0001485 - normal cellular response to oxidative stress

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Genotypes:

FYPO:0001788 - normal protein localization to cytoplasm

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Genotypes:

FYPO:0000838 - normal protein localization to nucleus during vegetative growth

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Genotypes:

FYPO:0001246 - normal RNA level during cellular response to hydrogen peroxide

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0005969 - resistance to magnesium chloride

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0009039 - resistance to potassium chloride

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000852 - resistance to salt stress

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0007921 - sensitive to benzamidine

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0000111 - sensitive to rapamycin

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Genotypes:

FYPO:0000271 - sensitive to salt stress

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0009063 - sensitive to X-rays during vegetative growth

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF08447PAS_3PAS_fold_3PFAM
PF00512HisKAHisK_dim/P_domPFAM
PF00072Response_regSig_transdc_resp-reg_receiverPFAM
PF13191AAA_16AAA_16PFAM
PF13185GAF_2GAFPFAM
PF00069PkinaseProt_kinase_domPFAM
cd16938HATPase_ETR2_ERS2-EIN4-likeCDD
cd00082HisKAHisK_dim/P_domCDD
cd17546REC_hyHK_CKI1_RcsC-likeCDD
cd00130PASPASCDD
PS50109HIS_KINHis_kinase_domPROSITE_PROFILES
PS50110RESPONSE_REGULATORYSig_transdc_resp-reg_receiverPROSITE_PROFILES
PS50011PROTEIN_KINASE_DOMProt_kinase_domPROSITE_PROFILES
PS50113PACPAS-assoc_CPROSITE_PROFILES
SM00388HisKA_10HisK_dim/P_domSMART
SM00220serkin_6Prot_kinase_domSMART
SM00448REC_2Sig_transdc_resp-reg_receiverSMART
SM00086pac_2PACSMART
G3DSA:1.10.287.130:FF:000002FUNFAM
G3DSA:3.30.450.40:FF:000044FUNFAM
G3DSA:3.30.450.20:FF:000099FUNFAM
SSF55781GAF domain-likeSUPERFAMILY
SSF55874ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinaseHATPase_C_sfSUPERFAMILY
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
SSF55785PYP-like sensor domain (PAS domain)PAS-like_dom_sfSUPERFAMILY
SSF52172CheY-likeCheY-like_superfamilySUPERFAMILY
SSF56112Protein kinase-like (PK-like)Kinase-like_dom_sfSUPERFAMILY
SSF47384Homodimeric domain of signal transducing histidine kinaseHisK_dim/P_sfSUPERFAMILY
G3DSA:1.10.287.130GENE3D
G3DSA:3.30.450.20PAS domainGENE3D
G3DSA:3.30.450.40GAF-like_dom_sfGENE3D
G3DSA:3.30.565.10HATPase_C_sfGENE3D
G3DSA:1.10.510.10Transferase(Phosphotransferase) domain 1GENE3D
G3DSA:3.40.50.2300GENE3D
PTHR43719TWO-COMPONENT HISTIDINE KINASE2C_system_His_kinasePANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

Orthologs

References / Literature

PMID:17559414 - Functional characterization of the phosphorelay protein Mpr1p from Schizosaccharomyces pombe.
Tan H et al. FEMS Yeast Res 2007 Sep;7(6):912-21
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:11758939 - Genetic analysis of the His-to-Asp phosphorelay implicated in mitotic cell cycle control: involvement of histidine-kinase genes of Schizosaccharomyces pombe.
Aoyama K et al. Biosci Biotechnol Biochem 2001 Oct;65(10):2347-52
PMID:20919928 - Two-component mediated peroxide sensing and signal transduction in fission yeast.
Quinn J et al. Antioxid Redox Signal 2011 Jul 01;15(1):153-65
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:12723602 - Characterization of the Prr1 response regulator with special reference to sexual development in Schizosaccharomyces pombe.
Nakamichi N et al. Biosci Biotechnol Biochem 2003 Mar;67(3):547-55
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:12596864 - His-to-Asp phosphorelay circuitry for regulation of sexual development in Schizosaccharomyces pombe.
Nakamichi N et al. Biosci Biotechnol Biochem 2002 Dec;66(12):2663-72
PMID:11179424 - Peroxide sensors for the fission yeast stress-activated mitogen-activated protein kinase pathway.
Buck V et al. Mol Biol Cell 2001 Feb;12(2):407-19
PMID:20625380 - A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length.
Liu NN et al. Cell Res 2010 Aug;20(8):963-5
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:27613427 - Stress sensitivity of a fission yeast strain lacking histidine kinases is rescued by the ectopic expression of Chk1 from Candida albicans.
Maksimov V et al. Curr Genet 2017 May;63(2):343-357