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protein coding gene - rrp45 (SPCC757.08) - exosome subunit Rrp45

Gene summary

Standard name
rrp45
Systematic ID
SPCC757.08
Product
exosome subunit Rrp45
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O74918
ORFeome ID
15/15E10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 62814..64464 forward strand

Annotation

Disease association

MONDO:0020135 - pontocerebellar hypoplasia

References:

MONDO:0016396 - pontocerebellar hypoplasia type 1

References:

MONDO:0054844 - pontocerebellar hypoplasia, type 1D

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GO biological process

GO:0000467 - exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

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GO:0070651 - nonfunctional rRNA decay

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GO:0071028 - nuclear mRNA surveillance

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GO:0071042 - nuclear polyadenylation-dependent mRNA catabolic process

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GO:0071035 - nuclear polyadenylation-dependent rRNA catabolic process

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GO:0070478 - nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay

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GO:0070481 - nuclear-transcribed mRNA catabolic process, non-stop decay

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GO:0071038 - TRAMP-dependent tRNA surveillance pathway

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GO:0034473 - U1 snRNA 3'-end processing

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GO:0034475 - U4 snRNA 3'-end processing

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GO:0034476 - U5 snRNA 3'-end processing

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GO cellular component

GO:0000177 - cytoplasmic exosome (RNase complex)

References:

GO:0005829 - cytosol

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GO:0000178 - exosome (RNase complex)

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GO:0000176 - nuclear exosome (RNase complex)

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GO:0005634 - nucleus

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GO molecular function

GO:0000175 - 3'-5'-RNA exonuclease activity

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GO:0035925 - mRNA 3'-UTR AU-rich region binding

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Modification

MOD:00046 - O-phospho-L-serine

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

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Genotypes:

FYPO:0000310 - inviable after spore germination, without cell division, with normal germ tube morphology

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01138RNase_PHExoRNase_PH_dom1Pfam
PF03725RNase_PH_CExoRNase_PH_dom2Pfam
cd11368RNase_PH_RRP45Rrp45CDD
G3DSA:3.30.230.70:FF:000005CATH-FunFam
G3DSA:3.30.230.70PNPase/RNase_PH_dom_sfCATH-Gene3D
SSF54211Ribosomal_Su5_D2-typ_SFSUPERFAMILY
SSF55666ExoRNase_PH_dom2_sfSUPERFAMILY
PTHR11097Exosome_comp_Rrp42_subfamPANTHER

Orthologs

References / Literature

PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:29727687 - Variants in EXOSC9 Disrupt the RNA Exosome and Result in Cerebellar Atrophy with Spinal Motor Neuronopathy.
Burns DT et al. Am J Hum Genet 2018 May 03;102(5):858-873
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PB_REF:0000003 - Disease Association Curation
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.
Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23755256 - The Sm complex is required for the processing of non-coding RNAs by the exosome.
Coy S et al. PLoS One 2013;8(6):e65606
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:29906447 - Helicase-Dependent RNA Decay Illuminated by a Cryo-EM Structure of a Human Nuclear RNA Exosome-MTR4 Complex.
Weick EM et al. Cell 2018 Jun 14;173(7):1663-1677.e21
PMID:24223771 - New insights into the RNA-based mechanism of action of the anticancer drug 5'-fluorouracil in eukaryotic cells.
Mojardín L et al. PLoS One 2013;8(11):e78172
PMID:23503588 - The exoribonuclease Dis3L2 defines a novel eukaryotic RNA degradation pathway.
Malecki M et al. EMBO J 2013 Jul 03;32(13):1842-54