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protein coding gene - vph2 (SPCC757.10) - ER membrane V-ATPase assembly factor Vph2/Vma12

Gene summary

Standard name
vph2
Systematic ID
SPCC757.10
Product
ER membrane V-ATPase assembly factor Vph2/Vma12
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
vma12
UniProt ID
O74920
ORFeome ID
07/07A11
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome III: 68252..69479 forward strand

Annotation

Disease association

MONDO:0014790 - TMEM199-CDG

References:

GO biological process

GO:0070072 - vacuolar proton-transporting V-type ATPase complex assembly

References:

GO cellular component

GO:0005783 - endoplasmic reticulum

References:

GO:0005789 - endoplasmic reticulum membrane

References:

Modification

MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0003559 - sensitive to doxorubicin

References:

Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0004413 - decreased phosphatase activity during cellular response to phosphate starvation

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

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Genotypes:

FYPO:0006680 - sensitive to bisphenol A

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Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

References:

Genotypes:

FYPO:0000098 - sensitive to calcium

References:

Genotypes:

FYPO:0003384 - sensitive to chromium

References:

Genotypes:

FYPO:0003559 - sensitive to doxorubicin

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF11712Vma12ATPase_Vma12PFAM
PTHR31394TRANSMEMBRANE PROTEIN 199ATPase_Vma12PANTHER

Orthologs

References / Literature

PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:30148840 - Tdp1 processes chromate-induced single-strand DNA breaks that collapse replication forks.
Ganguly A et al. PLoS Genet 2018 Aug;14(8):e1007595
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:23365689 - Cellular robustness conferred by genetic crosstalk underlies resistance against chemotherapeutic drug doxorubicin in fission yeast.
Tay Z et al. PLoS One 2013;8(1):e55041
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
PMID:26891792 - Calcium modulation of doxorubicin cytotoxicity in yeast and human cells.
Nguyen TT et al. Genes Cells 2016 Mar;21(3):226-40
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:37445861 - Resistance to Chemotherapeutic 5-Fluorouracil Conferred by Modulation of Heterochromatic Integrity through Ino80 Function in Fission Yeast.
Lim KK et al. Int J Mol Sci 2023 Jun 26;24(13)
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:25547512 - Dissection of the PHO pathway in Schizosaccharomyces pombe using epistasis and the alternate repressor adenine.
Estill M et al. Curr Genet 2015 May;61(2):175-83
PMID:21169418 - Systematic screen of Schizosaccharomyces pombe deletion collection uncovers parallel evolution of the phosphate signal transduction pathway in yeasts.
Henry TC et al. Eukaryot Cell 2011 Feb;10(2):198-206
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25669599 - Fitness profiling links topoisomerase II regulation of centromeric integrity to doxorubicin resistance in fission yeast.
Nguyen TT et al. Sci Rep 2015 Feb 11;5:8400
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:26791325 - Predicting chemotherapeutic drug combinations through gene network profiling.
Nguyen TT et al. Sci Rep 2016 Jan 21;6:18658
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105