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protein coding gene - gtr2 (SPCC777.05) - RagC/D GTPase Gtr2

Gene summary

Standard name
gtr2
Systematic ID
SPCC777.05
Product
RagC/D GTPase Gtr2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O74544
ORFeome ID
15/15E02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1605093..1606539 forward strand

Annotation

Disease association

MONDO:0859328 - hypomagnesemia 7, renal, with or without dilated cardiomyopathy

References:

MONDO:0957960 - Long-Olsen-Distelmaier syndrome

References:

GO biological process

GO:0009267 - cellular response to starvation

References:

GO:0010507 - negative regulation of autophagy

References:

GO:0110045 - negative regulation of cell cycle switching, mitotic to meiotic cell cycle

References:

GO:1904262 - negative regulation of TORC1 signaling

References:

GO:1904263 - positive regulation of TORC1 signaling

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0000329 - fungal-type vacuole membrane

References:

GO:1990131 - Gtr1-Gtr2 GTPase complex

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005525 - GTP binding

References:

GO:0003924 - GTPase activity

References:

GO:0043539 - protein serine/threonine kinase activator activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0003252 - abolished cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0004457 - decreased protein localization to nucleus during nitrogen starvation

References:

Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0000243 - normal growth on proline nitrogen source

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0001029 - resistance to canavanine

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0002128 - abolished protein localization to plasma membrane, with protein mislocalized to cytoplasm, during vegetative growth

References:

Genotypes:

FYPO:0004250 - abolished protein localization to vacuolar membrane

References:

Genotypes:

FYPO:0006148 - abolished transcription during nitrogen starvation

References:

Genotypes:

FYPO:0001575 - abolished vegetative cell population growth

References:

Genotypes:

FYPO:0000046 - decreased cell population growth

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0010009 - decreased protein localization to cell surface, with protein mislocalized to cytoplasm

References:

Genotypes:

FYPO:0004457 - decreased protein localization to nucleus during nitrogen starvation

References:

Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

References:

Genotypes:

FYPO:0005118 - decreased transcription during nitrogen starvation

References:

Genotypes:

FYPO:0000781 - decreased transcription during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0006345 - increased duration of protein phosphorylation during nitrogen starvation

References:

Genotypes:

FYPO:0002681 - increased protein phosphorylation during nitrogen starvation

References:

Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0002429 - inviable after spore germination, multiple cell divisions, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0000310 - inviable after spore germination, without cell division, with normal germ tube morphology

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0003031 - mating without nitrogen starvation

References:

Genotypes:

FYPO:0001522 - normal growth on caffeine

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0001545 - normal growth on L-canavanine

References:

Genotypes:

FYPO:0002672 - normal growth on rapamycin

References:

Genotypes:

FYPO:0008372 - normal protein localization to cell surface during vegetative growth

References:

Genotypes:

FYPO:0004248 - normal protein localization to vacuolar membrane

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

References:

Genotypes:

FYPO:0001029 - resistance to canavanine

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0000099 - sensitive to canavanine

References:

Genotypes:

FYPO:0000111 - sensitive to rapamycin

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF04670Gtr1_RagAGtr1_RagAPfam
cd11385RagC_likeRagC/DCDD
G3DSA:3.40.50.300:FF:000643CATH-FunFam
G3DSA:3.30.450.190CATH-Gene3D
G3DSA:3.40.50.300P-loop_NTPaseCATH-Gene3D
SSF52540P-loop_NTPaseSUPERFAMILY
PTHR11259Gtr1_RagAPANTHER

Orthologs

References / Literature

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:27227887 - The Loss of Lam2 and Npr2-Npr3 Diminishes the Vacuolar Localization of Gtr1-Gtr2 and Disinhibits TORC1 Activity in Fission Yeast.
Ma N et al. PLoS One 2016;11(5):e0156239
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:29199950 - Ragulator and GATOR1 complexes promote fission yeast growth by attenuating TOR complex 1 through Rag GTPases.
Chia KH et al. Elife 2017 Dec 04;6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:22344254 - The Vam6 and Gtr1-Gtr2 pathway activates TORC1 in response to amino acids in fission yeast.
Valbuena N et al. J Cell Sci 2012 Apr 15;125(Pt 8):1920-8
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:39010328 - Fission yeast Pib2 localizes to vacuolar membranes and regulates TOR complex 1 through evolutionarily conserved domains.
Morozumi Y et al. FEBS Lett 2024 Jul 15;
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23934889 - TORC1 signaling is governed by two negative regulators in fission yeast.
Ma N et al. Genetics 2013 Oct;195(2):457-68
PMID:26689777 - Tor Signaling Regulates Transcription of Amino Acid Permeases through a GATA Transcription Factor Gaf1 in Fission Yeast.
Ma Y et al. PLoS One 2015;10(12):e0144677
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24344203 - Isp7 is a novel regulator of amino acid uptake in the TOR signaling pathway.
Laor D et al. Mol Cell Biol 2014 Mar;34(5):794-806