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protein coding gene - tef101 (SPCC794.09c) - translation elongation factor EF-1 alpha Ef1a-a

Gene summary

Standard name
tef101
Systematic ID
SPCC794.09c
Product
translation elongation factor EF-1 alpha Ef1a-a
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ef1a-a, efa11, EF-1aA, tef1-e, ef1a-e
UniProt ID
P0CT53
ORFeome ID
44/44D05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 267955..269516 reverse strand

Annotation

Disease association

MONDO:0014625 - developmental and epileptic encephalopathy, 33

References:

MONDO:0014617 - intellectual disability, autosomal dominant 38

References:

GO biological process

GO:0002182 - cytoplasmic translational elongation

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GO cellular component

GO:0005829 - cytosol

References:

GO:0005853 - eukaryotic translation elongation factor 1 complex

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GO molecular function

GO:0005516 - calmodulin binding

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GO:0005525 - GTP binding

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GO:0003924 - GTPase activity

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GO:0003746 - translation elongation factor activity

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Modification

MOD:02025 - 5-glutaminyl glutamic acid

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MOD:00595 - mannosylated residue

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MOD:00663 - methylated lysine

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MOD:01982 - N,N,N-trimethylglycine

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MOD:00083 - N6,N6,N6-trimethyl-L-lysine

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MOD:00084 - N6,N6-dimethyl-L-lysine

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MOD:00085 - N6-methyl-L-lysine

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MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:00696 - phosphorylated residue

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MOD:01149 - sumoylated lysine

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MOD:01148 - ubiquitinylated lysine

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000027 - ribosomal density decreased

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PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000014 - RNA present

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000121 - abnormal sporulation

References:

Genotypes:

FYPO:0001118 - abnormal vegetative cell morphology

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Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009052 - increased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009074 - increased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009035 - resistance to formamide

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002767 - resistance to terbinafine

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0007921 - sensitive to benzamidine

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0000111 - sensitive to rapamycin

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Genotypes:

FYPO:0007938 - sensitive to tea tree oil

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0001510 - viable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF03144GTP_EFTU_D2EFTu-like_2PFAM
PF22594GTP-eEF1A_CGTP-eEF1A_CPFAM
PF00009GTP_EFTUT_Tr_GTP-bd_domPFAM
cd03705EF1_alpha_IIICDD
cd03693EF1_alpha_IICDD
cd01883EF1_alphaCDD
PS00301G_TR_1G_TR_CSPROSITE_PATTERNS
PS51722G_TR_2T_Tr_GTP-bd_domPROSITE_PROFILES
PR00315ELONGATNFCTT_Tr_GTP-bd_domPRINTS
G3DSA:2.40.30.10:FF:000003FUNFAM
G3DSA:3.40.50.300:FF:000211FUNFAM
G3DSA:2.40.30.10:FF:000005FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
SSF50447Translation proteinsTransl_B-barrel_sfSUPERFAMILY
SSF50465EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domainTransl_elong_EF1A/Init_IF2_CSUPERFAMILY
G3DSA:2.40.30.10Translation factorsGENE3D
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
PTHR23115TRANSLATION FACTORTRAFAC_GTPase_membersPANTHER
MF_00118_AEF_Tu_ATransl_elong_EF1A_euk/arcHAMAP
TIGR00483EF-1_alphaTransl_elong_EF1A_euk/arcNCBIFAM
NF008969PRK12317.1NCBIFAM

Orthologs

References / Literature

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:19336419 - Cotranscriptional recruitment of the nuclear poly(A)-binding protein Pab2 to nascent transcripts and association with translating mRNPs.
Lemieux C et al. Nucleic Acids Res 2009 Jun;37(10):3418-30
PMID:14534314 - Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent transcription in vitro.
Spahr H et al. J Biol Chem 2003 Dec 19;278(51):51301-6
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:25989903 - The fission yeast MTREC complex targets CUTs and unspliced pre-mRNAs to the nuclear exosome.
Zhou Y et al. Nat Commun 2015 May 20;6:7050
PMID:10526238 - Overproduction of elongation factor 1alpha, an essential translational component, causes aberrant cell morphology by affecting the control of growth polarity in fission yeast.
Suda M et al. Genes Cells 1999 Sep;4(9):517-27
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:26644575 - Discovery of a nucleocytoplasmic O-mannose glycoproteome in yeast.
Halim A et al. Proc Natl Acad Sci U S A 2015 Dec 22;112(51):15648-53
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:26542710 - Identification and characterization of Csh3 as an SH3 protein that interacts with fission yeast Cap1.
Yamamoto T et al. FEMS Yeast Res 2015 Dec;15(8)
PMID:10593605 - Cloning of a Schizosaccharomyces pombe homologue of elongation factor 1 alpha by two-hybrid selection of calmodulin-binding proteins.
Rasmussen C et al. Biochem Cell Biol 1999;77(5):421-30
PMID:28801462 - Protein glutaminylation is a yeast-specific posttranslational modification of elongation factor 1A.
Jank T et al. J Biol Chem 2017 Sep 29;292(39):16014-16023
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:36630955 - Zinc-finger protein Zpr1 is a bespoke chaperone essential for eEF1A biogenesis.
Sabbarini IM et al. Mol Cell 2023 Jan 19;83(2):252-265.e13
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:20403971 - Proteomic and functional analysis of the noncanonical poly(A) polymerase Cid14.
Keller C et al. RNA 2010 Jun;16(6):1124-9
PMID:26115316 - Saccharomyces cerevisiae Eukaryotic Elongation Factor 1A (eEF1A) Is Methylated at Lys-390 by a METTL21-Like Methyltransferase.
Jakobsson ME et al. PLoS One 2015;10(6):e0131426
PMID:36820394 - Inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast.
Sanchez AM et al. MicroPubl Biol 2023;2023
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:9099890 - Comprehensive cloning of Schizosaccharomyces pombe genes encoding translation elongation factors.
Mita K et al. Gene 1997 Mar 18;187(2):259-66
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
PMID:3214489 - Peptide elongation factor 1 from yeasts: purification and biochemical characterization of peptide elongation factors 1 alpha and 1 beta (gamma) from Saccharomyces carlsbergensis and Schizosaccharomyces pombe.
Miyazaki M et al. J Biochem 1988 Mar;103(3):508-21
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:31276588 - Inositol pyrophosphates impact phosphate homeostasis via modulation of RNA 3' processing and transcription termination.
Sanchez AM et al. Nucleic Acids Res 2019 Sep 19;47(16):8452-8469
PMID:19682301 - A large complex mediated by Moc1, Moc2 and Cpc2 regulates sexual differentiation in fission yeast.
Paul SK et al. FEBS J 2009 Sep;276(18):5076-93
PMID:18292091 - Global analysis of gel mobility of proteins and its use in target identification.
Shirai A et al. J Biol Chem 2008 Apr 18;283(16):10745-52
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24818994 - The S. pombe translation initiation factor eIF4G is Sumoylated and associates with the SUMO protease Ulp2.
Jongjitwimol J et al. PLoS One 2014;9(5):e94182
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24442611 - Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes.
Chen W et al. RNA 2014 Mar;20(3):308-20
PMID:23035257 - Targeting mitochondrial transcription in fission yeast with ETB, an inhibitor of HSP60, the chaperone that binds to the mitochondrial transcription factor Mtf1.
Sun W et al. Genes Cells 2012 Feb;17(2):122-31
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:31294478 - Senataxin homologue Sen1 is required for efficient termination of RNA polymerase III transcription.
Rivosecchi J et al. EMBO J 2019 Aug 15;38(16):e101955
GO_REF:0000002 - Comments
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399