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protein coding gene - gbs1 (SPCC825.02) - glucosidase II beta subunit

Gene summary

Standard name
gbs1
Systematic ID
SPCC825.02
Product
glucosidase II beta subunit
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
gls2beta
UniProt ID
Q9USH8
ORFeome ID
26/26D11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 1025524..1027919 forward strand

Annotation

Disease association

MONDO:0000447 - autosomal dominant polycystic liver disease

References:

MONDO:0008265 - polycystic liver disease 1

References:

GO biological process

GO:0006491 - N-glycan processing

References:

GO cellular component

GO:0005783 - endoplasmic reticulum

References:

GO:0005788 - endoplasmic reticulum lumen

References:

GO:0017177 - glucosidase II complex

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GO molecular function

GO:0005537 - D-mannose binding

References:

GO:0030234 - enzyme regulator activity

References:

GO:0140767 - enzyme-substrate adaptor activity

References:

GO:2001065 - mannan binding

References:

Modification

MOD:00689 - disulfide crosslinked residues

References:

Protein sequence feature

SO:0001806 - ER_retention_signal

SO:0000418 - signal_peptide

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0004862 - curved microtubules

References:

Genotypes:

FYPO:0000663 - decreased catalytic activity

References:

Genotypes:

FYPO:0007797 - decreased glucan 1,3-alpha-glucosidase activity

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0001686 - normal growth on carbendazim

References:

Genotypes:

FYPO:0000964 - normal growth on thiabendazole

References:

Genotypes:

FYPO:0003702 - normal microtubule cytoskeleton morphology during vegetative growth

References:

Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0009034 - resistance to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009067 - sensitive to amorolfine

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000107 - sensitive to latrunculin A

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF12999PRKCSH-likePRKCSH_NPfam
PF13015PRKCSH_1PRKCSHPfam
cd00112LDLaLDrepeatLR_classA_rptCDD
PS51914MRHMRH_domPROSITE profiles
G3DSA:2.70.130.10Man6P_isomerase_rcpt-bd_dom_sfCATH-Gene3D
SSF50911Man6P_isomerase_rcpt-bd_dom_sfSUPERFAMILY
PTHR12630Gtb1-likePANTHER
Signal PeptideDeepTMHMM-Signal-Peptide
CoilCOILS

Orthologs

References / Literature

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PB_REF:0000003 - Disease Association Curation
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:23609449 - Structure of the lectin mannose 6-phosphate receptor homology (MRH) domain of glucosidase II, an enzyme that regulates glycoprotein folding quality control in the endoplasmic reticulum.
Olson LJ et al. J Biol Chem 2013 Jun 07;288(23):16460-16475
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:26062005 - Crystal Structure and Functional Analyses of the Lectin Domain of Glucosidase II: Insights into Oligomannose Recognition.
Olson LJ et al. Biochemistry 2015 Jul 07;54(26):4097-111
PMID:19605557 - Glucosidase II beta subunit modulates N-glycan trimming in fission yeasts and mammals.
Stigliano ID et al. Mol Biol Cell 2009 Sep;20(17):3974-84
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:25552606 - Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. G3 (Bethesda) 2014 Dec 31;5(3):361-70
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:15797925 - The nuclear rim protein Amo1 is required for proper microtubule cytoskeleton organisation in fission yeast.
Pardo M et al. J Cell Sci 2005 Apr 15;118(Pt 8):1705-14
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160