protein coding gene - alp14 (SPCC895.07) - TOG/XMAP215 microtubule plus end tracking polymerase Alp14

Gene summary

Standard name

alp14

Systematic ID

SPCC895.07

Product

TOG/XMAP215 microtubule plus end tracking polymerase Alp14

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

mtc1

UniProt ID

O94534

ORFeome ID

28/28G03

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome III: 1011443..1014314 forward strand

Annotation

Protein features

Orthologs

• CKAP5 (HGNC:28959) Homo sapiens
• alp14 (SJAG_03540) Schizosaccharomyces japonicus
• STU2 (YLR045C) Saccharomyces cerevisiae

References / Literature

PMID:29021344 - A microtubule polymerase cooperates with the kinesin-6 motor and a microtubule cross-linker to promote bipolar spindle assembly in the absence of kinesin-5 and kinesin-14 in fission yeast.

Yukawa M et al. Mol Biol Cell 2017 Dec 01;28(25):3647-3659

PMID:12390246 - Cut1/separase C-terminus affects spindle pole body positioning in interphase of fission yeast: pointed nuclear formation.

Nakamura T et al. Genes Cells 2002 Nov;7(11):1113-24

PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.

Han TX et al. Genome Biol 2010;11(6):R60

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.

Mojardín L et al. Cell Cycle 2015;14(2):206-18

PMID:36108046 - Recovery from spindle checkpoint-mediated arrest requires a novel Dnt1-dependent APC/C activation mechanism.

Bai S et al. PLoS Genet 2022 Sep;18(9):e1010397

PMID:17442892 - The conserved Spc7 protein is required for spindle integrity and links kinetochore complexes in fission yeast.

Kerres A et al. Mol Biol Cell 2007 Jul;18(7):2441-54

PMID:14742702 - Interdependency of fission yeast Alp14/TOG and coiled coil protein Alp7 in microtubule localization and bipolar spindle formation.

Sato M et al. Mol Biol Cell 2004 Apr;15(4):1609-22

PMID:25210736 - Dynamics of cell shape inheritance in fission yeast.

Abenza JF et al. PLoS One 2014;9(9):e106959

PMID:25057016 - Csi1p recruits alp7p/TACC to the spindle pole bodies for bipolar spindle formation.

Zheng F et al. Mol Biol Cell 2014 Sep 15;25(18):2750-60

PMID:25472718 - Alp7/TACC recruits kinesin-8-PP1 to the Ndc80 kinetochore protein for timely mitotic progression and chromosome movement.

Tang NH et al. J Cell Sci 2015 Jan 15;128(2):354-63

PMID:26864000 - Alp7/TACC-Alp14/TOG generates long-lived, fast-growing MTs by an unconventional mechanism.

Hussmann F et al. Sci Rep 2016 Feb 11;6:20653

PMID:37783794 - An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity.

Ader NR et al. Nat Cell Biol 2023 Oct;25(10):1465-1477

PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.

Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71

PMID:22696680 - Fission yeast Alp14 is a dose-dependent plus end-tracking microtubule polymerase.

Al-Bassam J et al. Mol Biol Cell 2012 Aug;23(15):2878-90

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:36793083 - The SAGA histone acetyltransferase module targets SMC5/6 to specific genes.

Mahrik L et al. Epigenetics Chromatin 2023 Feb 16;16(1):6

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:19606211 - Nucleocytoplasmic shuttling of the TACC protein Mia1p/Alp7p is required for remodeling of microtubule arrays during the cell cycle.

Ling YC et al. PLoS One 2009 Jul 16;4(7):e6255

PMID:15371542 - The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association.

Kerres A et al. Mol Biol Cell 2004 Dec;15(12):5255-67

PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.

Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7

PMID:30422110 - Structural basis of tubulin recruitment and assembly by microtubule polymerases with tumor overexpressed gene (TOG) domain arrays.

Nithianantham S et al. Elife 2018 Nov 13;7

PMID:26744419 - Control of heterochromatin localization and silencing by the nuclear membrane protein Lem2.

Barrales RR et al. Genes Dev 2016 Jan 15;30(2):133-48

PMID:23770679 - Microtubules and Alp7-Alp14 (TACC-TOG) reposition chromosomes before meiotic segregation.

Kakui Y et al. Nat Cell Biol 2013 Jul;15(7):786-96

PMID:34346498 - Loss of kinesin-8 improves the robustness of the self-assembled spindle in Schizosaccharomyces pombe.

Pineda-Santaella A et al. J Cell Sci 2021 Aug 15;134(16)

PMID:31071203 - The concerted actions of Tip1/CLIP-170, Klp5/Kinesin-8, and Alp14/XMAP215 regulate microtubule catastrophe at the cell end.

Niu X et al. J Mol Cell Biol 2019 Dec 23;11(11):956-966

PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.

Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99

PMID:11369198 - M phase-specific kinetochore proteins in fission yeast: microtubule-associating Dis1 and Mtc1 display rapid separation and segregation during anaphase.

Nakaseko Y et al. Curr Biol 2001 Apr 17;11(8):537-49

PMID:31618856 - Two XMAP215/TOG Microtubule Polymerases, Alp14 and Dis1, Play Non-Exchangeable, Distinct Roles in Microtubule Organisation in Fission Yeast.

Yukawa M et al. Int J Mol Sci 2019 Oct 15;20(20)

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:11432827 - Fission yeast ch-TOG/XMAP215 homologue Alp14 connects mitotic spindles with the kinetochore and is a component of the Mad2-dependent spindle checkpoint.

Garcia MA et al. EMBO J 2001 Jul 02;20(13):3389-401

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:16920624 - CDC2 phosphorylation of the fission yeast dis1 ensures accurate chromosome segregation.

Aoki K et al. Curr Biol 2006 Aug 22;16(16):1627-35

PMID:21215368 - The Cul4-Ddb1(Cdt)² ubiquitin ligase inhibits invasion of a boundary-associated antisilencing factor into heterochromatin.

Braun S et al. Cell 2011 Jan 07;144(1):41-54

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.

Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86

PMID:23166349 - Csi1 links centromeres to the nuclear envelope for centromere clustering.

Hou H et al. J Cell Biol 2012 Nov 26;199(5):735-44

PMID:19696784 - Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast.

Sato M et al. EMBO Rep 2009 Oct;10(10):1161-7

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:30463883 - Suppressor Analysis Uncovers That MAPs and Microtubule Dynamics Balance with the Cut7/Kinesin-5 Motor for Mitotic Spindle Assembly in Schizosaccharomyces pombe .

Yukawa M et al. G3 (Bethesda) 2019 Jan 09;9(1):269-280

PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.

Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9

PMID:12242294 - The mal2p protein is an essential component of the fission yeast centromere.

Jin QW et al. Mol Cell Biol 2002 Oct;22(20):7168-83

PMID:27872152 - An unconventional interaction between Dis1/TOG and Mal3/EB1 in fission yeast promotes the fidelity of chromosome segregation.

Matsuo Y et al. J Cell Sci 2016 Dec 15;129(24):4592-4606

PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.

Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16

PMID:26124291 - A stable microtubule array drives fission yeast polarity reestablishment upon quiescence exit.

Laporte D et al. J Cell Biol 2015 Jul 06;210(1):99-113

PMID:21965289 - Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B.

Buttrick GJ et al. Mol Biol Cell 2011 Dec;22(23):4486-502

PMID:33925026 - Dual Impact of a Benzimidazole Resistant β-Tubulin on Microtubule Behavior in Fission Yeast.

Minagawa M et al. Cells 2021 Apr 28;10(5)

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.

Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82

PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.

Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183

PMID:34080538 - Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth.

Krüger LK et al. Elife 2021 Jun 03;10

PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.

Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.

Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160

PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.

Mak T et al. EMBO J 2021 Aug 16;40(16):e107911

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.

Das J et al. Sci Signal 2013 May 21;6(276):ra38

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:26098123 - Promoter nucleosome dynamics regulated by signalling through the CTD code.

Materne P et al. Elife 2015 Jun 22;4:e09008

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:25764183 - Condensin HEAT subunits required for DNA repair, kinetochore/centromere function and ploidy maintenance in fission yeast.

Xu X et al. PLoS One 2015;10(3):e0119347

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:9658169 - Identification of novel temperature-sensitive lethal alleles in essential beta-tubulin and nonessential alpha 2-tubulin genes as fission yeast polarity mutants.

Radcliffe P et al. Mol Biol Cell 1998 Jul;9(7):1757-71

PMID:26510788 - Escape from Mitotic Arrest: An Unexpected Connection Between Microtubule Dynamics and Epigenetic Regulation of Centromeric Chromatin in Schizosaccharomyces pombe.

George AA et al. Genetics 2015 Dec;201(4):1467-78

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:29958883 - Role of nucleocytoplasmic transport in interphase microtubule organization in fission yeast.

Kume K et al. Biochem Biophys Res Commun 2018 Sep 05;503(2):1160-1167

PMID:29779879 - The XMAP215 Ortholog Alp14 Promotes Microtubule Nucleation in Fission Yeast.

Flor-Parra I et al. Curr Biol 2018 Jun 04;28(11):1681-1691.e4

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.

Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480

PMID:30973898 - Mal3 is a multi-copy suppressor of the sensitivity to microtubule-depolymerizing drugs and chromosome mis-segregation in a fission yeast pka1 mutant.

Tanabe T et al. PLoS One 2019;14(4):e0214803

TreeFam:TF105639 - Unknown title

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:24790093 - CDK-dependent phosphorylation of Alp7-Alp14 (TACC-TOG) promotes its nuclear accumulation and spindle microtubule assembly.

Okada N et al. Mol Biol Cell 2014 Jul 01;25(13):1969-82

PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.

Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787

PMID:12426374 - Spindle-kinetochore attachment requires the combined action of Kin I-like Klp5/6 and Alp14/Dis1-MAPs in fission yeast.

Garcia MA et al. EMBO J 2002 Nov 15;21(22):6015-24

PMID:23427262 - The internal loop of fission yeast Ndc80 binds Alp7/TACC-Alp14/TOG and ensures proper chromosome attachment.

Tang NH et al. Mol Biol Cell 2013 Apr;24(8):1122-33

PMID:17476213 - Alp7/TACC is a crucial target in Ran-GTPase-dependent spindle formation in fission yeast.

Sato M et al. Nature 2007 May 17;447(7142):334-7

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:15464854 - MAPping the eukaryotic tree of life: structure, function, and evolution of the MAP215/Dis1 family of microtubule-associated proteins.

Gard DL et al. Int Rev Cytol 2004;239:179-272

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105