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protein coding gene - ast1 (SPCC962.05) - nuclease, XP-G family protein Ast1

Gene summary

Standard name
ast1
Systematic ID
SPCC962.05
Product
nuclease, XP-G family protein Ast1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O14061
ORFeome ID
35/35B08
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome III: 554323..556291 forward strand

Annotation

GO biological process

GO:0006281 - DNA repair

References:

GO cellular component

GO:0005634 - nucleus

References:

GO:0035861 - site of double-strand break

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GO molecular function

GO:0004518 - nuclease activity

References:

Modification

MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0002553 - abnormal double-strand break processing

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Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

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Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

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Genotypes:

FYPO:0002474 - decreased protein localization to double-strand break site

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Genotypes:

FYPO:0002897 - decreased protein phosphorylation during cellular response to DNA damage

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Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

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Genotypes:

FYPO:0002102 - normal mitotic DNA damage checkpoint during cellular response to UV

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Genotypes:

FYPO:0002899 - normal protein phosphorylation during cellular response to DNA damage

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

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Genotypes:

Protein features

PBO:0111762 - XP-G family

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0009007 - decreased vegetative cell population viability

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Genotypes:

FYPO:0004806 - incomplete cell wall disassembly at cell fusion site

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

References:

Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

Protein features

IDNameInterPro nameDB name
PF12813XPG_I_2Asteroid_domPfam
cd18675PIN_SpAst1-likeCDD
G3DSA:3.40.50.1010CATH-Gene3D
SSF88723PIN-like_dom_sfSUPERFAMILY
PTHR15665AsteroidPANTHER

Orthologs

References / Literature

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model
PMID:23211746 - Initiation of DNA damage responses through XPG-related nucleases.
Kuntz K et al. EMBO J 2013 Jan 23;32(2):290-302
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:34984977 - Functional profiling of long intergenic non-coding RNAs in fission yeast.
Rodriguez-Lopez M et al. Elife 2022 Jan 05;11