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protein coding gene - sec8 (SPCC970.09) - exocyst complex subunit Exo4

Gene summary

Standard name
sec8
Systematic ID
SPCC970.09
Product
exocyst complex subunit Exo4
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
exo4
UniProt ID
O74562
ORFeome ID
30/30E09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 490016..495933 reverse strand

Annotation

GO biological process

GO:0032120 - ascospore-type prospore membrane formation

References:

GO:0006887 - exocytosis

References:

GO cellular component

GO:1902716 - cell cortex of growing cell tip

References:

GO:0032153 - cell division site

References:

GO:0051286 - cell tip

References:

GO:0005737 - cytoplasm

References:

GO:0031410 - cytoplasmic vesicle

References:

GO:0005829 - cytosol

References:

GO:0000145 - exocyst

References:

GO:0090619 - meiotic spindle pole

References:

GO:0110085 - mitotic actomyosin contractile ring

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:7770062 - vesicle membrane tethering activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0001972 - abnormal cell separation after cytokinesis resulting in septated cell

References:

Genotypes:

FYPO:0004481 - abolished cell population growth at high temperature

References:

Genotypes:

FYPO:0004105 - abolished polar cell growth

References:

Genotypes:

FYPO:0001585 - abolished protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0000134 - branched, elongated, multiseptate cell

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0006883 - decreased exocytosis at cell division site

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0004653 - delayed onset of actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0000223 - elongated multiseptate vegetative cell

References:

Genotypes:

FYPO:0001122 - elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0007689 - normal cell growth polarization

References:

Genotypes:

FYPO:0001587 - normal protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

FYPO:0001581 - vacuolated

References:

Genotypes:

FYPO:0000647 - vegetative cell lysis

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001972 - abnormal cell separation after cytokinesis resulting in septated cell

References:

Genotypes:

FYPO:0000034 - abnormal endocytosis during vegetative growth

References:

Genotypes:

FYPO:0006497 - abnormal plasma membrane phosphatidylserine distribution

References:

Genotypes:

FYPO:0001370 - abnormal protein localization

References:

Genotypes:

FYPO:0004481 - abolished cell population growth at high temperature

References:

Genotypes:

FYPO:0003209 - abolished protein localization to cell tip, with protein mislocalized to cytoplasm

References:

Genotypes:

FYPO:0006558 - abolished septum disassembly

References:

Genotypes:

FYPO:0003440 - cell lysis during cytokinesis

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0006330 - decreased endoplasmic reticulum-plasma membrane tethering

References:

Genotypes:

FYPO:0004572 - decreased exocytosis during vegetative growth

References:

Genotypes:

FYPO:0005736 - decreased prospore membrane formation

References:

Genotypes:

FYPO:0002869 - decreased protein localization to cell division site

References:

Genotypes:

FYPO:0001586 - decreased protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0005552 - decreased protein localization to cleavage furrow rim

References:

Genotypes:

FYPO:0000538 - decreased protein secretion during vegetative growth

References:

Genotypes:

FYPO:0002088 - exocytic vesicles present in increased numbers at septum during septum assembly

References:

Genotypes:

FYPO:0001042 - inviable after spore germination, single or double cell division

References:

Genotypes:

FYPO:0000313 - inviable after spore germination, without cell division, with abnormal germ tube morphology

References:

Genotypes:

FYPO:0001494 - inviable elongated multiseptate vegetative cell

References:

Genotypes:

FYPO:0006556 - inviable elongated tetranucleate triseptate vegetative cell, single septa between nuclei

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002444 - loss of punctate cytoplasmic protein localization

References:

Genotypes:

FYPO:0002487 - normal actin cable morphology

References:

Genotypes:

FYPO:0001294 - normal actin cortical patch localization

References:

Genotypes:

FYPO:0001368 - normal actomyosin contractile ring assembly

References:

Genotypes:

FYPO:0004097 - normal actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0007689 - normal cell growth polarization

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0007676 - normal intracellular sterol transport

References:

Genotypes:

FYPO:0004963 - normal plasma membrane sterol distribution

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0000644 - normal protein localization during vegetative growth

References:

Genotypes:

FYPO:0001587 - normal protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0002558 - normal protein localization to medial cortex during vegetative growth

References:

Genotypes:

FYPO:0008003 - normal protein localization to site of mechanical stress

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF04048Sec8_NSec8_exocyst_NPfam
PF20652Sec8_CSec8_MPfam
PTHR14146Sec8/EXOC4PANTHER
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:25724972 - Rho4 interaction with exocyst and septins regulates cell separation in fission yeast.
Pérez P et al. Microbiology (Reading) 2015 May;161(Pt 5):948-959
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:27898700 - Sbg1 Is a Novel Regulator for the Localization of the β-Glucan Synthase Bgs1 in Fission Yeast.
Davidson R et al. PLoS One 2016;11(11):e0167043
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21899677 - Cdc42 regulates multiple membrane traffic events in fission yeast.
Estravís M et al. Traffic 2011 Dec;12(12):1744-58
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:34666001 - Detection of surface forces by the cell-wall mechanosensor Wsc1 in yeast.
Neeli-Venkata R et al. Dev Cell 2021 Oct 25;56(20):2856-2870.e7
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:30044717 - Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis.
Zhu YH et al. Mol Biol Cell 2018 Sep 15;29(19):2259-2279
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:22891673 - Fission yeast sec3 bridges the exocyst complex to the actin cytoskeleton.
Jourdain I et al. Traffic 2012 Nov;13(11):1481-95
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:41171630 - Septins function in exocytosis via physical interactions with the exocyst complex in fission yeast cytokinesis.
Singh D et al. Elife 2025 Oct 31;13
PMID:12930742 - Rho3p regulates cell separation by modulating exocyst function in Schizosaccharomyces pombe.
Wang H et al. Genetics 2003 Aug;164(4):1323-31
PMID:32320462 - Sterol biosensor reveals LAM-family Ltc1-dependent sterol flow to endosomes upon Arp2/3 inhibition.
Marek M et al. J Cell Biol 2020 Jun 01;219(6)
PMID:29930085 - Local and global Cdc42 guanine nucleotide exchange factors for fission yeast cell polarity are coordinated by microtubules and the Tea1-Tea4-Pom1 axis.
Tay YD et al. J Cell Sci 2018 Jul 19;131(14)
PMID:27630265 - The exocytic Rabs Ypt3 and Ypt2 regulate the early step of biogenesis of the spore plasma membrane in fission yeast.
Imada K et al. Mol Biol Cell 2016 Nov 01;27(21):3317-3328
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:21401840 - Pob1 ensures cylindrical cell shape by coupling two distinct rho signaling events during secretory vesicle targeting.
Nakano K et al. Traffic 2011 Jun;12(6):726-39
PMID:29290560 - ER-PM Contacts Restrict Exocytic Sites for Polarized Morphogenesis.
Ng AYE et al. Curr Biol 2018 Jan 08;28(1):146-153.e5
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:27082518 - Roles of the TRAPP-II Complex and the Exocyst in Membrane Deposition during Fission Yeast Cytokinesis.
Wang N et al. PLoS Biol 2016 Apr;14(4):e1002437
GO_REF:0000051 - S. pombe keyword mapping
PMID:21148300 - Actin cables and the exocyst form two independent morphogenesis pathways in the fission yeast.
Bendezú FO et al. Mol Biol Cell 2011 Jan 01;22(1):44-53
PMID:22891259 - Fission yeast Ags1 confers the essential septum strength needed for safe gradual cell abscission.
Cortés JC et al. J Cell Biol 2012 Aug 20;198(4):637-56
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:27852900 - Gradients of phosphatidylserine contribute to plasma membrane charge localization and cell polarity in fission yeast.
Haupt A et al. Mol Biol Cell 2017 Jan 01;28(1):210-220
PMID:25411334 - The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis.
Wang N et al. Mol Biol Cell 2015 Jan 15;26(2):238-55
PMID:16141239 - Endocytosis in fission yeast is spatially associated with the actin cytoskeleton during polarised cell growth and cytokinesis.
Gachet Y et al. J Cell Sci 2005 Sep 15;118(Pt 18):4231-42
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:24947517 - The putative exchange factor Gef3p interacts with Rho3p GTPase and the septin ring during cytokinesis in fission yeast.
Muñoz S et al. J Biol Chem 2014 Aug 08;289(32):21995-2007
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:11854409 - The multiprotein exocyst complex is essential for cell separation in Schizosaccharomyces pombe.
Wang H et al. Mol Biol Cell 2002 Feb;13(2):515-29
PMID:16415366 - A role for the septation initiation network in septum assembly revealed by genetic analysis of sid2-250 suppressors.
Jin QW et al. Genetics 2006 Apr;172(4):2101-12
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83