PomBase home

protein coding gene - brl2 (SPCC970.10c) - histone H2B-K119 ubiquitin ligase complex (HULC) subunit, ubiquitin-protein ligase E3 Brl2

Gene summary

Standard name
brl2
Systematic ID
SPCC970.10c
Product
histone H2B-K119 ubiquitin ligase complex (HULC) subunit, ubiquitin-protein ligase E3 Brl2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
rfp1
UniProt ID
O74563
ORFeome ID
37/37D01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 489781..493167 forward strand

Annotation

Comment

PBO:0000270 - chromatin assembly function of H2Bub is independent of H3K4 methylation

References:

PBO:0005161 - the chromatin silencing function of H2Bub is independent of H3K4 methylation

References:

GO biological process

GO:0007535 - donor selection

References:

GO:0040029 - epigenetic regulation of gene expression

References:

GO:0140673 - transcription elongation-coupled chromatin remodeling

References:

GO cellular component

GO:0033503 - HULC complex

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0140850 - histone H2B C-terminal K residue ubiquitin ligase activity

References:

GO:0008270 - zinc ion binding

References:

Modification

MOD:01149 - sumoylated lysine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0002913 - increased antisense RNA transcription

References:

Genotypes:

FYPO:0002360 - normal chromatin silencing at centromere

References:

Genotypes:

FYPO:0005072 - normal protein localization to centromeric chromatin

References:

Genotypes:

Protein features

PBO:0111749 - ubiquitin-protein ligase E3

PBO:0111746 - zf-C3HC4 type (RING finger)

PBO:0111743 - zinc finger protein

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000468 - abnormal mating type switching

References:

Genotypes:

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0002922 - abolished histone H2B-K119 ubiquitination during vegetative growth

References:

Genotypes:

FYPO:0001512 - branched, elongated cell

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0009075 - decreased cell population growth on fructose carbon source

References:

Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

References:

Genotypes:

FYPO:0003352 - decreased DNA double-strand break formation at mating-type locus

References:

Genotypes:

FYPO:0007478 - decreased epigenetic heterochromatin inheritance

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0003151 - decreased protein level during cellular response to heat

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0001122 - elongated vegetative cell

References:

Genotypes:

FYPO:0009077 - increased cell population growth on ethanol carbon source

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0009052 - increased cell population growth on glutamate nitrogen source

References:

Genotypes:

FYPO:0009101 - increased cell population growth on glycerol and galactose carbon source

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0009074 - increased cell population growth on serine nitrogen source

References:

Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0006299 - increased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0004557 - increased vegetative cell population growth

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

References:

Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0009030 - resistance to amitrole

References:

Genotypes:

FYPO:0009036 - resistance to benzamidine

References:

Genotypes:

FYPO:0009031 - resistance to bleomycin

References:

Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009034 - resistance to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0009070 - resistance to itraconazole

References:

Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0005969 - resistance to magnesium chloride

References:

Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

References:

Genotypes:

FYPO:0000077 - resistance to rapamycin

References:

Genotypes:

FYPO:0005266 - resistance to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000327 - resistance to trichostatin A

References:

Genotypes:

FYPO:0007808 - resistance to valproic acid

References:

Genotypes:

FYPO:0003595 - S-shaped cell

References:

Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

References:

Genotypes:

FYPO:0009067 - sensitive to amorolfine

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0001190 - sensitive to cell wall-degrading enzymes

References:

Genotypes:

FYPO:0002640 - sensitive to clotrimazole

References:

Genotypes:

FYPO:0001245 - sensitive to cobalt

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0007358 - sensitive to heavy water

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0002641 - sensitive to micafungin

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0005889 - sensitive to sodium chloride

References:

Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0007938 - sensitive to tea tree oil

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0009063 - sensitive to X-rays during vegetative growth

References:

Genotypes:

FYPO:0000647 - vegetative cell lysis

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002197 - viable vegetative cell with abnormal cell shape

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF08647BRE1PFAM
PF13923zf-C3HC4_2Znf_RINGPFAM
cd16499RING-HC_Bre1-likeCDD
PS00518ZF_RING_1Znf_RING_CSPROSITE_PATTERNS
PS50089ZF_RING_2Znf_RINGPROSITE_PROFILES
SM00184ring_2Znf_RINGSMART
SSF57850RING/U-boxSUPERFAMILY
G3DSA:3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)Znf_RING/FYVE/PHDGENE3D
PTHR23163RING FINGER PROTEIN-RELATEDE3_ubiquit_lig_Bre1PANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000043 - Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
PMID:37615341 - Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1 .
Budden AM et al. Elife 2023 Aug 24;12
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:38718864 - H2B oncohistones cause homologous recombination defect and genomic instability through reducing H2B monoubiquitination in Schizosaccharomyces pombe.
Qin B et al. J Biol Chem 2024 May 06;:107345
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.
Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:25373780 - A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.
Graml V et al. Dev Cell 2014 Oct 27;31(2):227-239
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:38181050 - The Cross-Regulation Between Set1, Clr4, and Lsd1/2 in Schizosaccharomyces pombe.
Liu H et al. PLoS Genet 2024 Jan 05;20(1):e1011107
PMID:25356590 - Multifaceted genome control by Set1 Dependent and Independent of H3K4 methylation and the Set1C/COMPASS complex.
Mikheyeva IV et al. PLoS Genet 2014 Oct;10(10):e1004740
PMID:32496538 - Cdk9 and H2Bub1 signal to Clr6-CII/Rpd3S to suppress aberrant antisense transcription.
Sansó M et al. Nucleic Acids Res 2020 Jul 27;48(13):7154-7168
PMID:17762865 - SUMO-targeted ubiquitin ligases in genome stability.
Prudden J et al. EMBO J 2007 Sep 19;26(18):4089-101
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:35858772 - The functional analysis of the ubiquitin ligase Brl2 in the repair of DNA double-strand breaks.
Liu XQ et al. Yi Chuan 2022 Jul 20;44(7):609-617
PMID:35908934 - Euchromatin factors HULC and Set1C affect heterochromatin organization and mating-type switching in fission yeast Schizosaccharomyces pombe.
Esquivel-Chávez A et al. Genes Genet Syst 2022 Oct 18;97(3):123-138
PMID:24385927 - The PAF complex and Prf1/Rtf1 delineate distinct Cdk9-dependent pathways regulating transcription elongation in fission yeast.
Mbogning J et al. PLoS Genet 2013;9(12):e1004029
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:17363370 - HULC, a histone H2B ubiquitinating complex, modulates heterochromatin independent of histone methylation in fission yeast.
Zofall M et al. J Biol Chem 2007 May 11;282(19):14065-72
PMID:22252817 - A genomewide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis.
Fang Y et al. Antimicrob Agents Chemother 2012 Apr;56(4):1949-59
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:32142608 - Mutations in a Single Signaling Pathway Allow Cell Growth in Heavy Water.
Kampmeyer C et al. ACS Synth Biol 2020 Apr 17;9(4):733-748
PMID:17374714 - Ubiquitylation of histone H2B controls RNA polymerase II transcription elongation independently of histone H3 methylation.
Tanny JC et al. Genes Dev 2007 Apr 01;21(7):835-47
PMID:29899117 - Ccp1 modulates epigenetic stability at centromeres and affects heterochromatin distribution in Schizosaccharomyces pombe .
Lu M et al. J Biol Chem 2018 Aug 03;293(31):12068-12080