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protein coding gene - mis18 (SPCC970.12) - kinetochore protein Mis18

Gene summary

Standard name
mis18
Systematic ID
SPCC970.12
Product
kinetochore protein Mis18
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9P802
ORFeome ID
07/07E03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome III: 513931..514807 reverse strand

Annotation

GO biological process

GO:0034080 - CENP-A containing chromatin assembly

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GO cellular component

GO:0098654 - CENP-A recruiting complex

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GO:0000785 - chromatin

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GO:0005737 - cytoplasm

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GO:0000776 - kinetochore

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GO:0005634 - nucleus

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GO molecular function

GO:0140463 - chromatin-protein adaptor activity

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GO:0005515 - protein binding

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GO:0008270 - zinc ion binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001387 - loss of viability at high temperature

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000141 - abnormal mitotic sister chromatid segregation

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Genotypes:

FYPO:0003786 - abolished protein localization to centromere central core during vegetative growth

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Genotypes:

FYPO:0000634 - abolished protein localization to centromere during vegetative growth

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Genotypes:

FYPO:0001269 - abolished protein localization to kinetochore during vegetative growth

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Genotypes:

FYPO:0000705 - abolished protein-protein interaction

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Genotypes:

FYPO:0003740 - decreased CENP-A containing chromatin assembly

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Genotypes:

FYPO:0002577 - decreased chromatin binding

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Genotypes:

FYPO:0006818 - decreased protein localization to chromatin at centromere outer repeat

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Genotypes:

FYPO:0002902 - decreased protein localization to kinetochore during vegetative growth

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Genotypes:

FYPO:0003415 - increased histone H4 acetylation at centromere central core during vegetative growth

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Genotypes:

FYPO:0000451 - increased protein localization to centromere during vegetative growth

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Genotypes:

FYPO:0002148 - inviable after spore germination, multiple cell divisions, abnormal morphology

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000284 - large and small daughter nuclei, with unequal mitotic sister chromatid segregation

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Genotypes:

FYPO:0001387 - loss of viability at high temperature

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Genotypes:

FYPO:0002574 - normal protein localization to centromere during vegetative growth

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Genotypes:

FYPO:0002901 - normal protein localization to kinetochore during vegetative growth

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

Subunit composition

PBO:0015212 - homomeric(2)

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Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF03226Yippee-Mis18Yippee/Mis18/CereblonPfam
PS51793MIS18Mis18PROSITE profiles
PTHR16431PANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:29804820 - Mis16 Switches Function from a Histone H4 Chaperone to a CENP-A Cnp1 -Specific Assembly Factor through Eic1 Interaction.
An S et al. Structure 2018 Jul 03;26(7):960-971.e4
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:26942680 - Licensing of Centromeric Chromatin Assembly through the Mis18α-Mis18β Heterotetramer.
Nardi IK et al. Mol Cell 2016 Mar 03;61(5):774-787
PMID:26921242 - Centromere localization and function of Mis18 requires Yippee-like domain-mediated oligomerization.
Subramanian L et al. EMBO Rep 2016 Apr;17(4):496-507
PMID:28977643 - Regulation of mitotic recombination between DNA repeats in centromeres.
Zafar F et al. Nucleic Acids Res 2017 Nov 02;45(19):11222-11235
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:29925533 - Fission Yeast CENP-C (Cnp3) Plays a Role in Restricting the Site of CENP-A Accumulation.
Suma M et al. G3 (Bethesda) 2018 Jul 31;8(8):2723-2733
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24789708 - Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly.
Subramanian L et al. Open Biol 2014 Apr 30;4(4):140043
PMID:17199038 - Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1.
Fujita Y et al. Dev Cell 2007 Jan;12(1):17-30
PMID:18362178 - Dissection of the essential steps for condensin accumulation at kinetochores and rDNAs during fission yeast mitosis.
Nakazawa N et al. J Cell Biol 2008 Mar 24;180(6):1115-31
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:19217404 - Fission yeast Scm3: A CENP-A receptor required for integrity of subkinetochore chromatin.
Pidoux AL et al. Mol Cell 2009 Feb 13;33(3):299-311
PMID:32318758 - Centromere targeting of Mis18 requires the interaction with DNA and H2A-H2B in fission yeast.
Zhang M et al. Cell Mol Life Sci 2021 Jan;78(1):373-384
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:28827290 - Kinetochore Components Required for Centromeric Chromatin Assembly Are Impacted by Msc1 in Schizosaccharomyces pombe .
Gao C et al. Genetics 2017 Oct;207(2):559-569
PMID:28904333 - The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3.
Choi ES et al. Nat Commun 2017 Sep 13;8(1):529
PMID:23028377 - Factors that promote H3 chromatin integrity during transcription prevent promiscuous deposition of CENP-A(Cnp1) in fission yeast.
Choi ES et al. PLoS Genet 2012 Sep;8(9):e1002985
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:15369671 - Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres.
Hayashi T et al. Cell 2004 Sep 17;118(6):715-29
PMID:17352737 - The fission yeast DASH complex is essential for satisfying the spindle assembly checkpoint induced by defects in the inner-kinetochore proteins.
Kobayashi Y et al. Genes Cells 2007 Mar;12(3):311-28
PMID:36200823 - The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary.
Tsunemine S et al. Nucleic Acids Res 2022 Oct 28;50(19):10914-10928
PMID:31371524 - Subunit interactions and arrangements in the fission yeast Mis16-Mis18-Mis19 complex.
Korntner-Vetter M et al. Life Sci Alliance 2019 Aug;2(4)
PMID:34460892 - Transcriptome sequencing and screening of genes related to glucose availability in Schizosaccharomyces pombe by RNA-seq analysis.
Tarhan Ç et al. Genet Mol Biol 2021;44(3):e20200245
PMID:24774534 - Schizosaccharomyces pombe centromere protein Mis19 links Mis16 and Mis18 to recruit CENP-A through interacting with NMD factors and the SWI/SNF complex.
Hayashi T et al. Genes Cells 2014 Jul;19(7):541-54
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:37714149 - Direct recruitment of Mis18 to interphase spindle pole bodies promotes CENP-A chromatin assembly.
London N et al. Curr Biol 2023 Oct 09;33(19):4187-4201.e6
PMID:19217403 - Fission yeast Scm3 mediates stable assembly of Cnp1/CENP-A into centromeric chromatin.
Williams JS et al. Mol Cell 2009 Feb 13;33(3):287-98
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25375240 - The kinetochore protein Kis1/Eic1/Mis19 ensures the integrity of mitotic spindles through maintenance of kinetochore factors Mis6/CENP-I and CENP-A.
Hirai H et al. PLoS One 2014;9(11):e111905