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snRNA gene - snu2 (SPSNRNA.02) - small nuclear RNA U2

Gene summary

Standard name
snu2
Systematic ID
SPSNRNA.02
Product
small nuclear RNA U2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
U2
Feature type
snRNA gene
Genomic location
chromosome I: 959370..959556 reverse strand

Annotation

Comment

PBO:0016901 - snu2 transcription termination requires hairpin structure cleaved by Pac1

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GO biological process

GO:0000395 - mRNA 5'-splice site recognition

References:

GO:0000348 - mRNA branch site recognition

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GO cellular component

GO:0005730 - nucleolus

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GO:0005634 - nucleus

References:

GO:0005681 - spliceosomal complex

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GO:0005686 - U2 snRNP

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GO molecular function

GO:0030627 - pre-mRNA 5'-splice site binding

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GO:0045131 - pre-mRNA branch point binding

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003556 - abnormal transcription termination

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Genotypes:

FYPO:0003597 - abolished snRNA 3'-end processing

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Genotypes:

FYPO:0003630 - abolished U2-type prespliceosome assembly

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Genotypes:

FYPO:0004204 - decreased mature snRNA level

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Genotypes:

FYPO:0003598 - decreased snRNA 3'-end processing

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Genotypes:

FYPO:0003599 - normal snRNA 3'-end processing

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Genotypes:

FYPO:0003161 - RNA absent from cell during vegetative growth

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Genotypes:

References / Literature

PMID:24298023 - Characterization and in vivo functional analysis of the Schizosaccharomyces pombe ICLN gene.
Barbarossa A et al. Mol Cell Biol 2014 Feb;34(4):595-605
PMID:28432181 - Dual nature of pseudouridylation in U2 snRNA: Pus1p-dependent and Pus1p-independent activities in yeasts and higher eukaryotes.
Deryusheva S et al. RNA 2017 Jul;23(7):1060-1067
PMID:20400941 - Specific splicing defects in S. pombe carrying a degron allele of the Survival of Motor Neuron gene.
Campion Y et al. EMBO J 2010 Jun 02;29(11):1817-29
PMID:2806913 - Cloning of Schizosaccharomyces pombe genes encoding the U1, U2, U3 and U4 snRNAs.
Dandekar T et al. Gene 1989 Sep 30;81(2):227-35
PMID:10409726 - Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing.
McDonald WH et al. Mol Cell Biol 1999 Aug;19(8):5352-62
PMID:3194197 - The sequence of U3 from Schizosaccharomyces pombe suggests structural divergence of this snRNA between metazoans and unicellular eukaryotes.
Porter GL et al. Nucleic Acids Res 1988 Nov 11;16(21):10131-52
GO_REF:0000115 - Automatic Gene Ontology annotation of non-coding RNA sequences through association of Rfam records with GO terms
PMID:3244367 - U2 small nuclear RNA is remarkably conserved between Schizosaccharomyces pombe and mammals.
Brennwald P et al. Mol Cell Biol 1988 Dec;8(12):5575-80
PMID:20152800 - Cleavage-induced termination in U2 snRNA gene expression.
Nabavi S et al. Biochem Biophys Res Commun 2010 Mar 12;393(3):461-5
PMID:23221635 - Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle.
Piazzon N et al. Nucleic Acids Res 2013 Jan;41(2):1255-72
PMID:23754748 - Splicing functions and global dependency on fission yeast slu7 reveal diversity in spliceosome assembly.
Banerjee S et al. Mol Cell Biol 2013 Aug;33(16):3125-36
PMID:10445882 - Pac1p, an RNase III homolog, is required for formation of the 3' end of U2 snRNA in Schizosaccharomyces pombe.
Zhou D et al. RNA 1999 Aug;5(8):1083-98
PMID:1943699 - Thirty-three nucleotides of 5' flanking sequence including the 'TATA' box are necessary and sufficient for efficient U2 snRNA transcription in Schizosaccharomyces pombe.
Dandekar T et al. Mol Microbiol 1991 Jul;5(7):1621-5
PMID:34352089 - Co-transcriptional RNA cleavage by Drosha homolog Pac1 triggers transcription termination in fission yeast.
Yague-Sanz C et al. Nucleic Acids Res 2021 Sep 07;49(15):8610-8624
PMID:26292707 - Structure of a yeast spliceosome at 3.6-angstrom resolution.
Yan C et al. Science 2015 Sep 11;349(6253):1182-91
PMID:2953599 - Fungal small nuclear ribonucleoproteins share properties with plant and vertebrate U-snRNPs.
Tollervey D et al. EMBO J 1987 Feb;6(2):469-76
PMID:33754639 - Identification and structural analysis of the Schizosaccharomyces pombe SMN complex.
Veepaschit J et al. Nucleic Acids Res 2021 Jul 21;49(13):7207-7223
PMID:17284461 - Biochemical and genetic analysis of RNA cap guanine-N2 methyltransferases from Giardia lamblia and Schizosaccharomyces pombe.
Hausmann S et al. Nucleic Acids Res 2007;35(5):1411-20
PMID:8809017 - Localization of modified nucleotides in Schizosaccharomyces pombe spliceosomal small nuclear RNAs: modified nucleotides are clustered in functionally important regions.
Gu J et al. RNA 1996 Sep;2(9):909-18
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:2192258 - Differential distribution of factors involved in pre-mRNA processing in the yeast cell nucleus.
Potashkin JA et al. Mol Cell Biol 1990 Jul;10(7):3524-34
PMID:12374752 - Pre-spliceosome formation in S.pombe requires a stable complex of SF1-U2AF(59)-U2AF(23).
Huang T et al. EMBO J 2002 Oct 15;21(20):5516-26
PMID:2406130 - A mutation in a single gene of Schizosaccharomyces pombe affects the expression of several snRNAs and causes defects in RNA processing.
Potashkin J et al. EMBO J 1990 Feb;9(2):525-34
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:2144896 - Immunoprecipitation distinguishes non-overlapping groups of snRNPs in Schizosaccharomyces pombe.
Tollervey D et al. Nucleic Acids Res 1990 Sep 11;18(17):5207-12