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Haploid genotype - cdc20-ct1deltaN(1-1217,Y1520N,D1625G,L1726S,S1904P aa) pol1-AID[Knockdown] spp2-AID[Knockdown]

Genotype summary

Description
cdc20-ct1deltaN(1-1217,Y1520N,D1625G,L1726S,S1904P aa) pol1-AID[Knockdown] spp2-AID[Knockdown]
Organism
Schizosaccharomyces pombe (fission yeast)

Allele summary

gene Product Allele name Description Type Expression
cdc20 (SPBC25H2.13c)DNA polymerase epsilon catalytic subunit Pol2cdc20-ct1deltaN1-1217,Y1520N,D1625G,L1726S,S1904Pamino_acid_deletion_and_mutationNot assayed or wild type
pol1 (SPAC3H5.06c)DNA polymerase alpha catalytic subunitpol1-AIDpol1-AIDfusion_or_chimeraKnockdown
spp2 (SPBC17D11.06)DNA primase large subunit Spp2spp2-AIDspp2-AIDfusion_or_chimeraKnockdown

Annotation

Multi-locus phenotype

FYPO:0006731 - decreased CMG complex progression from replication origin

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Genes:

FYPO:0006729 - decreased protein localization to chromatin distal to replication origin

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FYPO:0004961 - increased protein localization to chromatin at replication origin

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