id: gomodel:6a2b236300000163
title: "RNA polymerase II preinitiation complex assembly (GO:0051123), \ttranscription\
  \ initiation at RNA polymerase II promoter (GO:0006367) (TATA-less promoter)"
taxon: NCBITaxon:4896
status: production
date_modified: '2026-06-19'
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        - http://www.pombase.org
      term: GO:0005669
  molecular_function:
    type: MolecularFunctionAssociation
    term: GO:0140378
  occurs_in:
    type: CellularAnatomicalEntityAssociation
    evidence:
    - term: ECO:0007005
      reference: PMID:16823372
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-16'
        provided_by:
        - http://www.pombase.org
    provenances:
    - contributor:
      - https://orcid.org/0000-0001-6330-7526
      date: '2026-06-16'
      provided_by:
      - http://www.pombase.org
    term: GO:0005634
  part_of:
    type: BiologicalProcessAssociation
    evidence:
    - term: ECO:0000318
      reference: GO_REF:0000033
      with_objects:
      - PANTHER:PTN000262707
      - UniProtKB:Q16514
      - SGD:S000002552
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-16'
        provided_by:
        - http://www.pombase.org
    provenances:
    - contributor:
      - https://orcid.org/0000-0001-6330-7526
      date: '2026-06-16'
      provided_by:
      - http://www.pombase.org
    term: GO:0051123
- id: gomodel:6a2b236300000163/6a2b236300000173
  enabled_by:
    type: EnabledByGeneProductAssociation
    evidence:
    - term: ECO:0000314
      reference: PMID:12359329
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    - term: ECO:0000314
      reference: PMID:15182371
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    - term: ECO:0000269
      reference: PMID:8303296
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    provenances:
    - contributor:
      - https://orcid.org/0000-0001-6330-7526
      date: '2026-06-15'
      provided_by:
      - http://www.pombase.org
    term: PomBase:SPAC16E8.16
  molecular_function:
    type: MolecularFunctionAssociation
    term: GO:0016251
  occurs_in:
    type: CellularAnatomicalEntityAssociation
    evidence:
    - term: ECO:0007005
      reference: PMID:16823372
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    provenances:
    - contributor:
      - https://orcid.org/0000-0001-6330-7526
      date: '2026-06-15'
      provided_by:
      - http://www.pombase.org
    term: GO:0005634
  part_of:
    type: BiologicalProcessAssociation
    evidence:
    - term: ECO:0000501
      reference: GO_REF:0000002
      with_objects:
      - InterPro:IPR000812
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    - term: ECO:0000314
      reference: PMID:12359329
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    - term: ECO:0000318
      reference: GO_REF:0000033
      with_objects:
      - PANTHER:PTN000178621
      - UniProtKB:Q00403
      - PomBase:SPAC16E8.16
      - SGD:S000006290
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    provenances:
    - contributor:
      - https://orcid.org/0000-0001-6330-7526
      date: '2026-06-15'
      provided_by:
      - http://www.pombase.org
    term: GO:0051123
  causal_associations:
  - type: CausalAssociation
    evidence:
    - term: ECO:0000314
      reference: PMID:12359329
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    - term: ECO:0000314
      reference: PMID:15182371
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    - term: ECO:0000269
      reference: PMID:8303296
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    provenances:
    - contributor:
      - https://orcid.org/0000-0001-6330-7526
      date: '2026-06-15'
      provided_by:
      - http://www.pombase.org
    predicate: RO:0012009
    downstream_activity: gomodel:6a2b236300000163/6a2b236300000214
  - type: CausalAssociation
    evidence:
    - term: ECO:0000314
      reference: PMID:12359329
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    - term: ECO:0000314
      reference: PMID:15182371
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    - term: ECO:0000269
      reference: PMID:8303296
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    provenances:
    - contributor:
      - https://orcid.org/0000-0001-6330-7526
      date: '2026-06-15'
      provided_by:
      - http://www.pombase.org
    predicate: RO:0012009
    downstream_activity: gomodel:6a2b236300000163/6a2b236300000237
  - type: CausalAssociation
    evidence:
    - term: ECO:0000314
      reference: PMID:12359329
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    - term: ECO:0000314
      reference: PMID:15182371
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    - term: ECO:0000269
      reference: PMID:8303296
      provenances:
      - contributor:
        - https://orcid.org/0000-0001-6330-7526
        date: '2026-06-15'
        provided_by:
        - http://www.pombase.org
    provenances:
    - contributor:
      - https://orcid.org/0000-0001-6330-7526
      date: '2026-06-15'
      provided_by:
      - http://www.pombase.org
    predicate: RO:0002304
    downstream_activity: gomodel:6a2b236300000163/6a2b236300000282
molecules:
- id: gomodel:6a2b236300000163/6a2b236300000412
  term: PomBase:SPBC8D2.03c
- id: gomodel:6a2b236300000163/6a2b236300000408
  term: PomBase:SPAC1834.04
- id: gomodel:6a2b236300000163/6a2b236300000409
  term: PomBase:SPBC8D2.04
- id: gomodel:6a2b236300000163/6a2b236300000410
  term: PomBase:SPBC1105.11c
- id: gomodel:6a2b236300000163/6a2b236300000411
  term: PomBase:SPAC1834.03c
- id: gomodel:6a2b236300000163/6a2b236300000313
  term: PomBase:SPBC28F2.12
objects:
- id: GO:0016251
  label: RNA polymerase II general transcription initiation factor activity
  type: gocam:Object
- id: GO:0005672
  label: transcription factor TFIIA complex
  type: gocam:Object
- id: GO:0051123
  label: RNA polymerase II preinitiation complex assembly
  type: gocam:Object
- id: GO:0005634
  label: nucleus
  type: gocam:Object
- id: ECO:0000305
  label: curator inference used in manual assertion
  type: gocam:Object
- id: PomBase:SPCC553.11c
  label: toa2 Spom
  type: gocam:Object
- id: PomBase:SPBC28F2.09
  label: toa1 Spom
  type: gocam:Object
- id: PomBase:SPAC16E8.16
  label: sua7 Spom
  type: gocam:Object
- id: ECO:0000314
  label: direct assay evidence used in manual assertion
  type: gocam:Object
- id: ECO:0000269
  label: experimental evidence used in manual assertion
  type: gocam:Object
- id: ECO:0000501
  label: evidence used in automatic assertion
  type: gocam:Object
- id: ECO:0000318
  label: biological aspect of ancestor evidence used in manual assertion
  type: gocam:Object
- id: ECO:0007005
  label: high throughput direct assay evidence used in manual assertion
  type: gocam:Object
- id: PomBase:SPAC29E6.08
  label: tbp1 Spom
  type: gocam:Object
- id: GO:0005674
  label: transcription factor TFIIF complex
  type: gocam:Object
- id: PomBase:SPCC1620.09c
  label: tfg1 Spom
  type: gocam:Object
- id: PomBase:SPBC1198.13c
  label: tfg2 Spom
  type: gocam:Object
- id: PomBase:SPAC22H12.02
  label: tfg3 Spom
  type: gocam:Object
- id: GO:0003887
  label: DNA-directed DNA polymerase activity
  type: gocam:Object
- id: GO:0005665
  label: RNA polymerase II, core complex
  type: gocam:Object
- id: GO:0006367
  label: transcription initiation at RNA polymerase II promoter
  type: gocam:Object
- id: PomBase:SPBC28F2.12
  label: rpb1 Spom
  type: gocam:Object
- id: PomBase:SPAC1B3.12c
  label: rpb10 Spom
  type: gocam:Object
- id: PomBase:SPAC3A12.07
  label: rpb11 Spom
  type: gocam:Object
- id: PomBase:SPAC23G3.01
  label: rpb2 Spom
  type: gocam:Object
- id: PomBase:SPCC1442.10c
  label: rpb3 Spom
  type: gocam:Object
- id: PomBase:SPBC337.14
  label: rpb4 Spom
  type: gocam:Object
- id: PomBase:SPAC23C4.15
  label: rpb5 Spom
  type: gocam:Object
- id: PomBase:SPCC1020.04c
  label: rpb6 Spom
  type: gocam:Object
- id: PomBase:SPACUNK4.06c
  label: rpb7 Spom
  type: gocam:Object
- id: PomBase:SPBC14C8.12
  label: rpb8 Spom
  type: gocam:Object
- id: PomBase:SPAPYUG7.04c
  label: rpb9 Spom
  type: gocam:Object
- id: PomBase:SPBC19C2.03
  label: rpc10 Spom
  type: gocam:Object
- id: PomBase:SPAC3A12.05c
  label: taf2 Spom
  type: gocam:Object
- id: GO:0005673
  label: transcription factor TFIIE complex
  type: gocam:Object
- id: PomBase:SPAC458.07
  label: tfa1 Spom
  type: gocam:Object
- id: PomBase:SPCC1672.08c
  label: tfa2 Spom
  type: gocam:Object
- id: GO:0043139
  label: 5'-3' DNA helicase activity
  type: gocam:Object
- id: GO:0000439
  label: transcription factor TFIIH core complex
  type: gocam:Object
- id: GO:0001113
  label: transcription open complex formation at RNA polymerase II promoter
  type: gocam:Object
- id: PomBase:SPAC16E8.11c
  label: tfb1 Spom
  type: gocam:Object
- id: PomBase:SPBC13G1.13
  label: tfb2 Spom
  type: gocam:Object
- id: PomBase:SPBC30B4.07c
  label: tfb4 Spom
  type: gocam:Object
- id: PomBase:SPBC32F12.15
  label: tfb5 Spom
  type: gocam:Object
- id: PomBase:SPCC1494.09c
  label: tfb6 Spom
  type: gocam:Object
- id: PomBase:SPCC1682.07
  label: ssl1 Spom
  type: gocam:Object
- id: PomBase:SPAC17A5.06
  label: ptr8 Spom
  type: gocam:Object
- id: PomBase:SPAC1D4.12
  label: rad15 Spom
  type: gocam:Object
- id: GO:0140836
  label: RNA polymerase II CTD heptapeptide repeat S5 kinase activity
  type: gocam:Object
- id: PomBase:SPBC19F8.07
  label: mcs6 Spom
  type: gocam:Object
- id: GO:0060261
  label: positive regulation of transcription initiation by RNA polymerase II
  type: gocam:Object
- id: ECO:0000315
  label: mutant phenotype evidence used in manual assertion
  type: gocam:Object
- id: GO:0061575
  label: cyclin-dependent protein serine/threonine kinase activator activity
  type: gocam:Object
- id: PomBase:SPBP16F5.02
  label: mcs2 Spom
  type: gocam:Object
- id: GO:0004402
  label: histone acetyltransferase activity
  type: gocam:Object
- id: PomBase:SPAC2G11.14
  label: taf1 Spom
  type: gocam:Object
- id: PomBase:SPAC1834.04
  label: hht1 Spom
  type: gocam:Object
- id: PomBase:SPBC8D2.04
  label: hht2 Spom
  type: gocam:Object
- id: PomBase:SPBC1105.11c
  label: hht3 Spom
  type: gocam:Object
- id: PomBase:SPAC1834.03c
  label: hhf1 Spom
  type: gocam:Object
- id: PomBase:SPBC8D2.03c
  label: hhf2 Spom
  type: gocam:Object
- id: GO:0140378
  label: protein complex scaffold activity
  type: gocam:Object
- id: PomBase:SPCC5E4.03c
  label: taf5 Spom
  type: gocam:Object
- id: ECO:0000266
  label: sequence orthology evidence used in manual assertion
  type: gocam:Object
- id: ECO:0000250
  label: sequence similarity evidence used in manual assertion
  type: gocam:Object
- id: PomBase:SPCC16C4.18c
  label: taf6 Spom
  type: gocam:Object
- id: PomBase:SPAC12G12.05c
  label: taf9 Spom
  type: gocam:Object
- id: GO:0005198
  label: structural molecule activity
  type: gocam:Object
- id: PomBase:SPAC23G3.09
  label: taf4 Spom
  type: gocam:Object
- id: PomBase:SPAC15A10.02
  label: taf12 Spom
  type: gocam:Object
- id: PomBase:SPAC13F5.02c
  label: taf7 Spom
  type: gocam:Object
- id: PomBase:SPCC1259.06
  label: taf8 Spom
  type: gocam:Object
- id: PomBase:SPBC21H7.02
  label: taf10 Spom
  type: gocam:Object
- id: PomBase:SPAC1002.04c
  label: taf11 Spom
  type: gocam:Object
- id: GO:0005669
  label: transcription factor TFIID complex
  type: gocam:Object
- id: PomBase:SPCC1494.02c
  label: taf13 Spom
  type: gocam:Object
provenances:
- contributor:
  - https://orcid.org/0000-0001-6330-7526
  date: '2026-06-19'
  provided_by:
  - http://www.pombase.org
